FastQCFastQC Report
Wed 25 May 2016
SRR1781236_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781236_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94815
Sequences flagged as poor quality0
Sequence length76
%GC89

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5587258.927384907451355No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7710.8131624742920424No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4420.466170964509835No Hit
CTTATACACATCTGACGCTGCCGACGAGAATGATCGTGTAGATCTCGGTG3300.348046195222275No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3080.32484311554079No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1930.2035542899330275No Hit
GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1520.16031218689025997No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1470.15503875968992248No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1200.1265622528081No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1080.11390602752728998No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATGC150.002218595270.000011
TTGGGCG252.8209233E-670.02
GCGTCTG352.9467265E-760.08
GTCTGGC252.3798246E-455.99999610
CTTGGGC252.3798246E-455.9999961
GTTGTGC252.3798246E-455.9999961
CGTCTGG452.6682756E-854.4444479
GGCGTCT200.006932475652.57
CCGCCAA200.006932475652.523
CTCTAAT200.006932475652.542
TCTAATA200.006932475652.543
TGTGGGC200.006932475652.53
TCTCTAA200.006932475652.541
CGCGTCT200.006932475652.57
TGTGCGC200.006932475652.53
GTCTGGG352.0728752E-550.010
TCTGGCG506.120899E-848.99999611
TTGTGCG305.851997E-446.6666682
GGCGCGT404.5628236E-543.755
TCTGGGG404.5628236E-543.7511