FastQCFastQC Report
Wed 25 May 2016
SRR1781236_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781236_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94815
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT3062032.29446817486685No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG56855.995886726783737No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC25182.655697938089965No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC9080.9576543795812898No Hit
CTTAAACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT7780.820545272372515No Hit
CTTATAAACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT6610.6971470758846174No Hit
ATTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT6570.6929283341243474No Hit
ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT6490.6844908506038074Illumina PCR Primer Index 9 (95% over 21bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6340.6686705690027949No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGACGT5890.6212097241997574No Hit
CATATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT4880.5146864947529399No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCAGT4010.42292886146706743No Hit
CTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC3970.41871011970679745No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCTAAGATCTCGTATGCCGT3380.35648367874281495No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGCTCTCGTATGCCGT3270.3448821389020724No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGATAAGATCTCGTATGCCGT2900.305858777619575No Hit
CTTATACACATCTCCGAGCCAACGAGACTTGCTAAGATCTCGTATGCCGT2320.24468702209565996No Hit
CTTTACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC2280.24046828033539No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGCTCTCGTATGCCG2220.23414016769498497No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGACG2200.23203079681485No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCAG2110.22253862785424244No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCAAAGATCTCGTATGCCGT2100.22148394241417496No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC2000.21093708801349997No Hit
CTTATACACATCTCCGAGACCACGAGACTTGCTAAGATCTCGTATGCCGT1940.20460897537309497No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTTAGATCTCGTATGCCGT1930.2035542899330275No Hit
CTTATACACATCTCCGCGCCCACGAGACTTGCTAAGATCTCGTATGCCGT1930.2035542899330275No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG1850.19511680641248746No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATATCGTATGCCGT1840.19406212097241998No Hit
TTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC1810.19089806465221748No Hit
TATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCT1720.18140589569160998No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGA1680.17718715393134No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCTAAGATCTCGTATGCCGT1650.1740230976111375No Hit
CCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG1620.170859041290935No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG1390.14660127616938248No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTAAGCCGT1250.13183568000843748No Hit
CTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT1180.124452881927965No Hit
CTTATACACATCTCCGAGCACACGAGACTTGCTAAGATCTCGTATGCCGT1080.11390602752728998No Hit
TCTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT1020.10757791488688498No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGCTCTCGTATGCC990.10441385856668249No Hit
CTTATACACATCTCAGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT960.10124980224647999No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCACGTATGCCGT950.1001951168064125No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTA207.8233126E-570.1220947
CGAATTA150.002203300870.1220948
CGACTTA150.002203300870.1220948
TATGCCT150.002207926570.0850543
GTATTCC150.002207926570.0850542
TAAGACG150.002207926570.0850543
TCAGCTA301.0056647E-770.0850553
TCTGCCG150.002207926570.0850543
AAGACGT150.002207926570.0850544
ATGCCTT150.002207926570.0850544
ATGCTAG150.002207926570.0850554
ATTCCGT150.002207926570.0850544
TATTCCG207.8438345E-570.08504543
TACAGCA150.002212559370.0480452
TGCTTCA207.8643985E-570.0480455
AATAAGC150.002212559370.0480451
GAAAACA207.885006E-570.0110860
ATGAAAC150.002217199770.0110858
TGAAACA207.885006E-570.0110859
TGAAAAC207.885006E-570.0110859
Produced by FastQC (version 0.11.5)