FastQCFastQC Report
Wed 25 May 2016
SRR1781235_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781235_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences195753
Sequences flagged as poor quality0
Sequence length76
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4101620.952935587194066No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5450.27841208053005573No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2770.141504855608854No Hit
CTTATACACATCTGACGCTGCCGACGAGAATGATCGTGTAGATCTCGGTG2620.13384213779610019No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1960.10012617941998335No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGCCG200.006943466652.543
CGCGTGT200.006943466652.57
AGTGTCC200.006943466652.58
GGTAGTA200.006943466652.540
GCCGTAT750.051.33333254
GTATCAT901.8189894E-1242.7777857
TGGTCGC1150.042.60869649
GTATCAG503.466057E-642.01
CCGTATC953.6379788E-1240.52631455
TATGCAG350.001253218440.011
ATCTCGG901.03682396E-1038.8888942
CGTATCA1007.2759576E-1238.556
ATCATTA1100.038.18181659
TATCATT1051.2732926E-1136.66666458
TCTCGGT1051.2732926E-1136.66666443
TCGGTGG1250.036.39999845
AGCGTGT400.002414549535.044
CTTGGGG400.002414549535.01
CGTCGGT400.002414549535.023
CGGTGGT1253.6379788E-1233.646