FastQCFastQC Report
Wed 25 May 2016
SRR1781235_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781235_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences195753
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT141347.22032357103084No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG33231.6975474194520648No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC11350.579812314498373No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGACGT8900.4546545902233938No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTAGTATGCCGT7130.3642345200328986No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5620.28709649405117676No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCAGGATCTCGTATGCCGT5580.2850531026344424No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC5130.2620649491961809No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGACGGATCTCGTATGCCGT3400.1736882704224201No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTAGTATGCCG3270.16704724831803344No Hit
CTTATAAACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT3050.1558085955259945No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGA2920.14916757342160783No Hit
ATTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT2920.14916757342160783No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTAAGCCGT2850.14559163844232273No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGACG2570.13128789852518225No Hit
CTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTC2560.13077705067099868No Hit
ATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGTCTT2410.12311433285824483RNA PCR Primer, Index 30 (95% over 22bp)
CTTAAACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT2410.12311433285824483No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATTCCGT2250.11494076719130741No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTTA252.8245213E-670.0319548
ATTACGA207.8943536E-570.03194444
CGATTCC150.002222622769.9961728
ATCGTAA301.0202348E-769.9961739
CGTATCT352.9642615E-759.9967134
ACGTAAG352.9642615E-759.9967140
AGGGGGG11350.059.5121670
CCTATGC308.344314E-658.34504341
CGAAATA553.45608E-1157.29886648
ATTCCGA252.3807467E-456.0255644
CCGAAAT509.713403E-1056.0255647
AAATCGT252.3867679E-455.99693737
AATCGTA252.3867679E-455.99693738
TATCGTA1100.054.08794838
TAAGCCG1750.054.0108443
CTCGAAT1500.053.6637339
TCCGTAA200.00693094652.5239646
ACGAAAT200.00693094652.5239647
CGAATGA608.367351E-1152.51053641
AGATCCG200.0069449752.49712431
Produced by FastQC (version 0.11.5)