FastQCFastQC Report
Wed 25 May 2016
SRR1781234_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781234_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences314947
Sequences flagged as poor quality0
Sequence length76
%GC68

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8592927.283638199443082No Hit
CTTATACACATCTGACGCTGCCGACGAGAATGATCGTGTAGATCTCGGTG10770.34196229841846404No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9880.3137035755222307No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7620.24194547019022247No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4530.1438337244044236No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATACC150.002223400470.0000160
TTGTGCG550.070.02
ATATCGG401.063745E-861.24999642
TATCGGT401.063745E-861.24999643
GCCGTAT2850.056.49122654
CGTATCA2800.056.2556
CCGTATC2750.056.055
TGTGCGC653.6379788E-1253.8461533
GTATCAT2800.052.557
CGTCTGG1000.052.4999969
CTAACGT200.006947375452.49999630
ACTTTTG200.006947375452.4999968
CGAAGTT200.006947375452.49999640
CGCCGTA2950.052.2033953
TAGATCT2850.050.35087639
GTAGATC2800.050.038
AGATCTC2900.048.2758640
TCTCGGT3000.047.83333243
ATCATTA3000.047.83333259
TATCATT2950.047.45762658
Produced by FastQC (version 0.11.5)