FastQCFastQC Report
Wed 25 May 2016
SRR1781234_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781234_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences314947
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT4802615.24891489679216No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG75372.393101061448434No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC43241.37292941352037No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20690.6569359289023233No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGACGT15550.49373386633306554No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC12850.4080051564231442No Hit
CTTAAACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT11070.3514877106306775No Hit
CTTATAAACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT9240.2933826961361753No Hit
ATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTCTT7880.25020082744080746Illumina PCR Primer Index 10 (95% over 23bp)
ATTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT7390.23464265416085883No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCAGT6990.2219421045445742No Hit
CATATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT6020.1911432717250839No Hit
CTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC5340.1695523373774Illumina PCR Primer Index 10 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGA4780.1517715679146015No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTAGTATGCCGT4640.14732637554890188No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTAAGCCGT4430.14065858700035244No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGAATATCTCGTATGCCGT4040.1282755511244749No Hit
CTTATACACATCTCCGAGCCCACGAGACAGAAGATTATCTCGTATGCCGT3920.12446538623958953No Hit
CTTATACACATCTCCGAGCCAACGAGACAGTAGATTATCTCGTATGCCGT3680.11684505646981871No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGACG3680.11684505646981871No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGATAATCTCGTATGCCGT3330.10573207555556965No Hit
CTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG3320.10541456181516254No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTAAT150.002217743370.0449747
ATACCGT252.8280683E-670.03384444
CGAATTT150.00222474169.98936534
CGATAGA150.00222474169.98936537
AGGGGGG38550.062.81794770
CGACTAA352.9558214E-760.03854448
AATCTCG2200.057.2640236
AACCGTC252.3826637E-456.02707345
AATATCG252.3901567E-455.99149336
GCCGTAT2400.053.99299646
GTAGATA2800.053.74183330
CCGTATT2350.053.65146647
GATAATC2650.052.8221633
GACGTAA200.006929747852.53372646
CCGAATA200.006929747852.53372647
GACGACT407.4338095E-752.53372646
CCCGTTT200.006934104552.5253842
CGTAAGC2800.052.50869841
CCGTCTT75850.051.89884647
GCCGTAA1150.051.7723746
Produced by FastQC (version 0.11.5)