FastQCFastQC Report
Wed 25 May 2016
SRR1781233_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781233_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences251632
Sequences flagged as poor quality0
Sequence length76
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4894219.449831499968205No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6440.2559292935715648No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3890.15459083105487378No Hit
CTTATACACATCTGACGCTGCCGACGAGAATGATCGTGTAGATCTCGGTG3250.12915686399186113No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGACT200.006945758652.543
TGGCTAC200.006945758652.512
CCTTGTC200.006945758652.59
GCCGTAT800.048.12554
CCGTATC901.03682396E-1038.8888955
CGCCGTA1007.2759576E-1238.50000453
CGTATCA1003.237801E-1035.00000456
GGTTATA501.7066956E-435.0000041
ACGCCAA400.002415855235.023
GATATGA400.002415855235.066
TAGATCT1500.032.66666839
TCGGTGG1305.456968E-1232.30769345
TCTCGGT1357.2759576E-1231.11111343
AGCGATA450.00430212931.11111327
TGGTCGC1357.2759576E-1231.11111349
GTAGATC1500.030.33333438
CGGTGGT1700.028.8235346
TCGCCGT1352.564775E-1028.5185252
TCATTAA1352.564775E-1028.5185260
CATTAAA1352.564775E-1028.5185261
Produced by FastQC (version 0.11.5)