FastQCFastQC Report
Wed 25 May 2016
SRR1781233_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781233_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences251632
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT2607910.363944172442297No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG44381.7636866535257836No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC27041.0745851084122846No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC7920.3147453424047816No Hit
CTTATAAACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT6890.2738125516627456No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGACGT6720.2670566541616329No Hit
ATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTCTT6470.25712151077764356RNA PCR Primer, Index 24 (95% over 23bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6250.24837858459973294No Hit
CTTAAACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT6170.24519933871685637No Hit
ATTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT5530.2197653716538437No Hit
CTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC4430.17605074076429073No Hit
CATATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT4010.15935969987918866No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCAGT3790.15061677370127804No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGAATCTCGTATGCCGT2840.11286322884211865No Hit
CTTTACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGTC2600.10332549119348891RNA PCR Primer, Index 24 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTT150.002224707569.985521
CGTATAG150.002224707569.985533
AACTCGA207.9247344E-569.985537
ACGACTT453.8016879E-1062.27120247
AGGGGGG22900.060.2058670
TCGAAAG352.9667353E-759.99948540
ATATCGA308.3714385E-658.32124737
GCCGTAT750.056.04408346
TCGAATG1150.054.7821440
CGTCTTA1750.054.04250748
AAATCCG200.00695145352.4891210
ATGCGTG200.00695145352.489123
CGAATGC1150.051.77985841
CCGTCTT45750.051.0674947
TGCCGTA1100.050.94916545
GCCGTCT47000.050.4545846
CTTGAAA50100.050.31897757
TATGCCG50700.050.22688343
GCTTGAA49500.050.22155856
GTATGCC50850.050.14760642