FastQCFastQC Report
Wed 25 May 2016
SRR1781232_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781232_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152686
Sequences flagged as poor quality0
Sequence length76
%GC83

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6777244.38651873780176No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7490.4905492317566771No Hit
CTTATACACATCTGACGCTGCCGACGAGAATGATCGTGTAGATCTCGGTG5770.37789974195407566No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4940.3235398137353785No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3370.22071440734579462No Hit
GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1700.11133961201419908No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1650.10806491754319321No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1600.10479022307218736No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCTTC150.002221199770.0000160
ATATCGG150.002221199770.0000142
GATATCG150.002221199770.0000141
TTTGGGA150.002221199770.000012
GTAGATA207.906648E-570.038
TAGATAT252.8291997E-670.039
AGATATC308.345472E-658.33333640
CTTGTGG200.006940552552.51
CAGCGTG200.006940552552.512
ACTACAC200.006940552552.53
TGCACTC200.006940552552.542
TTCCTTC200.006940552552.542
ATCTGGC200.006940552552.510
ACTGGTT200.006940552552.534
CATCTGG305.863306E-446.6666689
ATCATTA900.046.66666459
GCCGTAT1050.046.66666454
CGTATCA1000.045.49999656
TATCATT950.044.2105358
TCATTAA950.044.2105360