FastQCFastQC Report
Wed 25 May 2016
SRR1781232_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781232_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152686
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT3338721.866444860694497No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG69844.574093237100978No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC25961.70022136934624No Hit
CTTATAAACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT11250.7368062559763174No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC10760.70471425016046No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10740.7034043723720577No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT8990.5887900658868527TruSeq Adapter, Index 12 (95% over 23bp)
CTTAAACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT8160.5344301376681556No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGACGT8060.5278807487261439No Hit
ATTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT7360.4820350261320619No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTAGAAATCTCGTATGCCGT5980.3916534587323003No Hit
CTTATACACATCTCCGAGCCCACGAGACTACATGAAATCTCGTATGCCGT5230.34253304166721243No Hit
CATATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT5100.3340188360425972No Hit
CTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC4620.30258176912094104TruSeq Adapter, Index 12 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCAGT4250.2783490300354977No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGACG3270.21416501840378294No Hit
CTTATACACATCTCCGAGCCAACGAGACTACTTGAAATCTCGTATGCCGT3240.2122002017211794No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCAG3210.21023538503857592No Hit
CTTATACACATCTCCGAGCCCACGAGACTAATTGAAATCTCGTATGCCGT3040.199101423837156No Hit
CTTTACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC3020.19779154604875362RNA PCR Primer, Index 12 (95% over 21bp)
CTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG2730.1787983181169197No Hit
TTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC2500.16373472355029275TruSeq Adapter, Index 12 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATATCGTATGCCGT2470.16176990686768924No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGA2380.15587545681987872No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC2340.15325570124307403No Hit
CCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG2070.1355723510996424No Hit
TATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCT1740.11395936759100375RNA PCR Primer, Index 12 (95% over 22bp)
CTTATACACATCTCCGAGACCACGAGACTACTTGAAATCTCGTATGCCGT1710.11199455090840024No Hit
TCTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT1670.10937479533159557No Hit
CTTATACACATCTCCGCGCCCACGAGACTACTTGAAATCTCGTATGCCGT1630.10675503975479089No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACTCC150.002219097270.0167137
TAAAGTA207.897298E-570.0167138
ACTGATA207.897298E-570.0167153
ATATGCT150.002219097270.0167152
TACTGAT252.8251889E-670.0167152
AATTCTG150.002219097270.0167150
TAATGAT150.002219097270.0167152
TCGAAAG150.002219097270.0167140
CTACTTT150.002221983469.99377428
TACTTTA150.002221983469.99377429
TAGACTA150.002221983469.99377424
TTTAAAT207.91013E-569.99377432
CATTGAA150.002221983469.99377456
ATCAGAG207.91013E-569.99377412
ATACTAG150.002221983469.99377428
AGAAATA353.6870915E-969.99377433
ATATCAG150.002221983469.99377410
CACTAGA150.002221983469.99377421
CTTTACA500.069.9937741
ACTTTAA150.002221983469.99377430
Produced by FastQC (version 0.11.5)