FastQCFastQC Report
Wed 25 May 2016
SRR1781231_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781231_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences286482
Sequences flagged as poor quality0
Sequence length76
%GC72

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8539329.807457362068124No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8840.3085708700721162No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7630.2663343595758198No Hit
CTTATACACATCTGACGCTGCCGACGAGAATGATCGTGTAGATCTCGGTG4120.14381357292953834No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4110.143464510859321No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2930.10227518657367654No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGATA150.002223194870.040
CGTATCA800.061.24999656
GCCGTAT800.056.87499654
CCTACAG200.00694673852.49999659
GTCTATG200.00694673852.4999962
TTCCGGA200.00694673852.49999638
TCGTCAA200.00694673852.49999650
CGCGTGT407.4644595E-752.4999967
CCGTATC950.051.57894555
TTGGGCG707.2759576E-1250.02
CTTGTGC506.179653E-848.9999961
TTGTGCG651.8553692E-1048.461542
GTATCAT950.047.89473357
GGTCTAT305.8719737E-446.6666641
CATTAAA901.8189894E-1242.7777861
TGTAGAT1600.039.37499637
TAGATCT1500.037.33333239
TGTGCGC852.564775E-937.0588263
CGGTGGT2100.036.66666846
GTTGTGC501.7072857E-435.01