FastQCFastQC Report
Wed 25 May 2016
SRR1781231_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781231_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences286482
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT4734016.524598404088213TruSeq Adapter, Index 5 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG74032.584106505818865TruSeq Adapter, Index 5 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC40991.4308054258208196RNA PCR Primer, Index 5 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGACGT12610.4401672705440482No Hit
TCTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC10930.38152484274753734RNA PCR Primer, Index 5 (95% over 21bp)
CTTAAACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT9390.3277692839340692RNA PCR Primer, Index 5 (95% over 23bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8690.30333493901885633No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCACGTATGCCGT8410.2935612010527712No Hit
ATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTCTT7860.27436278719081825TruSeq Adapter, Index 5 (96% over 26bp)
ATTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT7200.2513246905564748No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATATCGTATGCCGT6750.2356168973966951No Hit
CTTATAAACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT6400.22339972493908866No Hit
CTTATACACATCTCCGCGCCCACGAGACACAAAGTGATCTCGTATGCCGT5720.19966350416431047TruSeq Adapter, Index 5 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCAGT5590.19512569725148526No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAAGAGATCTCGTATGCCGT5270.18395571100453084No Hit
CATATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT5160.18011602823214024TruSeq Adapter, Index 5 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTAAGCCGT4790.16720073163409918No Hit
CTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGTC4790.16720073163409918TruSeq Adapter, Index 5 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACAAAAAGTGATCTCGTATGCCGT4280.14939856605301555No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTAGTATGCCGT3900.13613420738475715No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGA3860.13473795910388786TruSeq Adapter, Index 5 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGCTCTCGTATGCCGT3330.11623766938236957No Hit
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGACG2900.10122800036302455No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATCGA150.002223546269.9972137
GTTATCT150.002225086369.9849934
ATCGATA150.002225086369.9849934
TAATCGA150.002225086369.9849932
AGGGGGG37700.063.20925570
GACGAAT352.9509647E-760.05002246
CTTTATA2350.058.072652
TCTTTAT2400.056.8628041
ATTACGT252.3781808E-456.0466944
TATACCG252.3781808E-456.0466943
ATACCGT252.3781808E-456.0466944
TAAGCCG2750.056.04668843
TTTATAC2650.055.45983
CCGTAAT1400.055.04585647
GTCTTAA1100.054.11709649
GCCGTAA1300.053.8910546
CCGTCTA1250.053.24435447
GAATATA200.0069334552.52541451
ACGAATG800.052.50707240
CACGTAA800.052.49790639
Produced by FastQC (version 0.11.5)