FastQCFastQC Report
Wed 25 May 2016
SRR1781230_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781230_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90121
Sequences flagged as poor quality0
Sequence length76
%GC89

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5275058.5324175275463No Hit
CTTATACACATCTGACGCTGCCGACGATCCGTGCCGTGTAGATCTCGGTG11681.2960353302781815No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6240.69240243672396No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3340.3706128427336581No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2570.2851721574327848No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2450.271856725957324No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1580.17531984776023346No Hit
GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1520.16866213202250308No Hit
TCTTATACACATCTGACGCTGCCGACGATCCGTGCCGTGTAGATCTCGGT1240.13759279191309462No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1050.11651002541028174No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1050.11651002541028174No Hit
GCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1020.11318116754141655No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGGCG301.0135955E-770.0000111
TTGTGCG252.8197846E-670.02
GTGTGGC252.8197846E-670.010
CGCGTGT207.8890895E-569.999997
GCGTGTG401.0524673E-861.2499968
CTTGTGC308.317837E-658.3333361
TTGGGCG252.3791882E-455.9999962
TGTGCGC252.3791882E-455.9999963
CGTGTGG452.6662747E-854.4444479
TGCGCGT200.00693136652.4999965
GTATCAA200.00693136652.49999657
GCAGTAT200.00693136652.49999654
CGCAGTA200.00693136652.49999653
TCGCAGT200.00693136652.49999652
CGGCTGG200.00693136652.4999969
TTGTGGG200.00693136652.4999962
ACGTGTA200.00693136652.49999634
GGTCGCA200.00693136652.49999650
GTGCTGG200.00693136652.49999635
CATTAAA2400.051.04166861
Produced by FastQC (version 0.11.5)