FastQCFastQC Report
Wed 25 May 2016
SRR1781230_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781230_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90121
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT2214424.571409549383606No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG59396.590028961063458No Hit
CTTAAACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT51635.728964392316996No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22202.463354822960242No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC20342.2569656350906No Hit
CTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT13181.4624782237214413No Hit
CTAAAACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT8880.985341929184097No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC7900.8765992388011673No Hit
TCATATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG5460.605852132133465No Hit
CTCTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC2890.3206799747006802No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATATCGTATGCCGT2800.3106934010940846No Hit
CTTATACACATCTCCGCGCCCACGAGACTTTCGTCAATCTCGTATGCCGT2650.2940491117497587No Hit
ATACCCATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT2640.2929394924601369RNA PCR Primer, Index 48 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGACGT2560.28406253814316307No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGACAATCTCGTATGCCGT2510.2785144416950544No Hit
CTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC2490.27629520311581096No Hit
CTTATACACATCTCCGAGCCCACGAGACTATCGTCAATCTCGTATGCCGT2430.2696374873780806No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCC2230.24744510158564598No Hit
ATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCTT2220.24633548229602426RNA PCR Primer, Index 48 (95% over 23bp)
ATTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT2160.23967776655829387No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCAGT2020.22414309650358963No Hit
TTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTC1850.2052795685800202No Hit
CTTAAACACATCTCCGAGCCCACGAGACTTTCGTCAATATCGTATGCCGT1820.201950710711155No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCAG1690.1875256599460725No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTCGTAAATCTCGTATGCCGT1640.18197756349796385No Hit
CTTAAACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGACGT1630.18086794420834212No Hit
CTTAAACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCAGT1580.17531984776023346No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG1560.17310060918099No Hit
CCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG1520.16866213202250308No Hit
TATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGTCT1450.16089479699515097RNA PCR Primer, Index 48 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTTACGTCAATCTCGTATGCCGT1400.1553467005470423No Hit
ATTAAACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGT1330.1475793655196902No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATATCGTATGCCG1280.14203126907158153No Hit
CTTATACACATCTCCGAGCCCACGAGACATTCGTCAATCTCGTATGCCGT1160.12871583759612076No Hit
TCTAATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCG1130.12538697972725557No Hit
CTTAAACACATCTCCGCGCCCACGAGACTTTCGTCAATCTCGTATGCCGT1110.12316774114801213No Hit
CTTAAACACATCTCCGAGCCCACGAGACTATCGTCAATCTCGTATGCCGT1080.11983888327914693No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGACG1060.11761964469990346No Hit
TCTTATACACATCTCCGCGCCCACGAGACTTTCGTCAATCTCGTATGCCG1000.11096192896217308No Hit
CTTAAACACATCTCCGAGCCCACGAGACTTTCGTCAATCTCGTATGCCGA970.10763307109330789No Hit
CTTAAACACATCTCCGAGCCCACGAGACTTTCGTCAAACTCGTATGCCGT970.10763307109330789No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATGCC150.002214399270.0305442
CAGTCTA150.002214399270.0305447
CCTATGC207.87204E-570.0305441
AGCTTCA150.002214399270.0305455
GAAGTCT301.01053956E-770.0305446
TAAGACG252.8124778E-670.0305443
GAATGAC252.8124778E-670.0305442
ACATCAG150.002214399270.0305450
GTATACC207.87204E-570.0305442
CTGAATG150.002214399270.0305454
CCTCTTC252.8124778E-670.0305448
AAGACGT252.8124778E-670.0305444
TGCTTTA207.87204E-570.0305455
TTCTTGA150.002214399270.0305455
CGAATGA301.01053956E-770.0305441
TCTGAAT207.87204E-570.0305453
TCCATGA150.002214399270.0305455
GTAAGAC207.87204E-570.0305442
CGAAAGC252.8124778E-670.0305441
CGAAAGA150.002214399270.0305441
Produced by FastQC (version 0.11.5)