FastQCFastQC Report
Wed 25 May 2016
SRR1781229_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781229_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150878
Sequences flagged as poor quality0
Sequence length76
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1808011.983191717811742No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3240.21474303742096265No Hit
CTTATACACATCTGACGCTGCCGACGATCCGTGCCGTGTAGATCTCGGTG2980.1975105714550829No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTAAC200.006940393752.51
TAAGTAC200.006940393752.528
TCGGCTA200.006940393752.515
GTAATAG200.006940393752.51
CTCTAGT200.006940393752.54
TTCCTAT200.006940393752.536
ACTGGAC200.006940393752.565
CGTATCA750.051.3333356
CCGTATC651.8371793E-1048.46153655
GCCGTAT651.8371793E-1048.46153654
GTATCAT757.4760464E-1041.99999657
CAGCGGA350.001252304440.09
TCCATGG350.001252304440.02
CAGACAT350.001252304440.05
GTAGATC901.03682396E-1038.88888538
CGGTGGT951.8553692E-1036.84210646
GTGGTCG1300.035.048
TGGTCGC1102.3646862E-1135.049
CGCCGTA957.550625E-933.15789853
TCGCCGT1206.366463E-1132.08333652
Produced by FastQC (version 0.11.5)