##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1781229_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 150878 Sequences flagged as poor quality 0 Sequence length 76 %GC 61 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.68015217593022 32.0 27.0 32.0 13.0 32.0 2 28.192360715279897 32.0 27.0 32.0 13.0 32.0 3 27.749698431845598 32.0 27.0 32.0 13.0 32.0 4 26.572800540834315 32.0 27.0 32.0 13.0 32.0 5 27.47540396876947 32.0 27.0 32.0 13.0 32.0 6 31.42497249433317 37.0 32.0 37.0 13.0 37.0 7 30.952153395458584 37.0 27.0 37.0 13.0 37.0 8 31.25290632166386 37.0 32.0 37.0 13.0 37.0 9 31.15804159652169 37.0 27.0 37.0 13.0 37.0 10-11 30.835625472235847 37.0 27.0 37.0 10.5 37.0 12-13 30.794429936770108 37.0 27.0 37.0 13.0 37.0 14-15 31.63773711210382 37.0 29.5 37.0 13.0 37.0 16-17 31.76122430042816 37.0 29.5 37.0 13.0 37.0 18-19 31.009779424435635 37.0 27.0 37.0 13.0 37.0 20-21 31.440882699929745 37.0 29.5 37.0 13.0 37.0 22-23 31.364841129919537 37.0 29.5 37.0 13.0 37.0 24-25 30.64542212913745 37.0 27.0 37.0 13.0 37.0 26-27 31.209596495181536 37.0 29.5 37.0 13.0 37.0 28-29 30.692248041463962 37.0 27.0 37.0 13.0 37.0 30-31 30.88700473230027 37.0 27.0 37.0 13.0 37.0 32-33 30.881596389135595 37.0 27.0 37.0 13.0 37.0 34-35 30.819595964951816 37.0 27.0 37.0 13.0 37.0 36-37 31.551309667413406 37.0 29.5 37.0 13.0 37.0 38-39 31.343270722040323 37.0 29.5 37.0 13.0 37.0 40-41 30.568598470287252 37.0 29.5 37.0 10.5 37.0 42-43 30.63437678124047 37.0 27.0 37.0 13.0 37.0 44-45 30.319533000172324 37.0 27.0 37.0 13.0 37.0 46-47 30.66461975901059 37.0 29.5 37.0 13.0 37.0 48-49 31.140295470512598 37.0 29.5 37.0 13.0 37.0 50-51 31.376804438022774 37.0 32.0 37.0 13.0 37.0 52-53 30.348148835482974 37.0 27.0 37.0 13.0 37.0 54-55 30.50860297723989 37.0 27.0 37.0 13.0 37.0 56-57 30.267719614522992 37.0 27.0 37.0 10.5 37.0 58-59 29.803964129959304 37.0 24.5 37.0 10.5 37.0 60-61 29.5573112050796 37.0 22.0 37.0 8.0 37.0 62-63 29.244452471533293 37.0 22.0 37.0 8.0 37.0 64-65 29.057662482270445 37.0 22.0 37.0 8.0 37.0 66-67 29.198295974230838 37.0 22.0 37.0 8.0 37.0 68-69 29.595683267275547 37.0 22.0 37.0 8.0 37.0 70-71 29.705437505799388 37.0 22.0 37.0 8.0 37.0 72-73 29.961866541178964 37.0 27.0 37.0 10.5 37.0 74-75 30.292928723869615 37.0 27.0 37.0 13.0 37.0 76 30.066152785694403 37.0 27.0 37.0 13.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 11101 1 0.22674282841536808 11101 2 -0.36904629169625736 11101 3 0.0060696975939933395 11101 4 -2.4331066324671973 11101 5 -1.00680659930795 11101 6 -0.024577533251392225 11101 7 -2.0324903117536905 11101 8 -1.7869949857916971 11101 9 -0.5769659060043395 11101 10-11 -1.867398270059038 11101 12-13 -1.2226513338249347 11101 14-15 -1.5415934025366838 11101 16-17 -1.042470854201536 11101 18-19 -0.29617911797481256 11101 20-21 -0.7479521243719276 11101 22-23 -2.1552605835204766 11101 24-25 -1.5813708298923537 11101 26-27 -0.05408372820629026 11101 28-29 -0.7316529487688221 11101 30-31 0.13280358486414556 11101 32-33 -2.246701103205524 11101 34-35 0.2918736428045534 11101 36-37 -0.9252616392634287 11101 38-39 -0.40001029014131007 11101 40-41 -0.86288271663798 11101 42-43 -0.5137127226894158 11101 44-45 -1.346949643877224 11101 46-47 -1.1561999091223285 11101 48-49 -2.5255104634744576 11101 50-51 -0.7936080776246328 11101 52-53 -0.9854885697577593 11101 54-55 -0.6083976393511854 11101 56-57 -1.4140756936321175 11101 58-59 -1.4948417028083405 11101 60-61 -0.7355316605902011 11101 62-63 -1.4268667151183223 11101 64-65 0.24077027167405873 11101 66-67 0.40101877220694604 11101 68-69 1.029659660423583 11101 70-71 -1.7714855163383056 11101 72-73 -0.388473300718136 11101 74-75 0.45542690990577483 11101 76 -1.9391384937956957 11102 1 -0.5502174406617826 11102 2 0.1548132836663676 11102 3 -0.5114833820780689 11102 4 -0.6967181523326573 11102 5 -1.111704644294285 11102 6 -0.6403017291731921 11102 7 1.274215595330606 11102 8 0.6313358950155319 11102 9 -1.0316642452938076 11102 10-11 -1.303597555322856 11102 12-13 -1.6508623912140479 11102 14-15 -1.5630354878845907 11102 16-17 0.12065716766098333 11102 18-19 0.02650324485890465 11102 20-21 -0.03552832786152038 11102 22-23 -0.5174636526817125 11102 24-25 -0.6364475589232548 11102 26-27 -1.6082144828836071 11102 28-29 -0.4194814120860393 11102 30-31 -0.9791367136433173 11102 32-33 -0.2328268126862909 11102 34-35 0.21945405062461276 11102 36-37 -0.26476132393864305 11102 38-39 -1.1724509039735587 11102 40-41 -0.516550994964657 11102 42-43 -1.865615182222733 11102 44-45 -0.5973175227925083 11102 46-47 -1.0396348471084558 11102 48-49 -0.12778080192305907 11102 50-51 0.06470598586076193 11102 52-53 -0.1563946029719716 11102 54-55 1.067763806938494 11102 56-57 -3.2806933097767477 11102 58-59 -1.5985835569180722 11102 60-61 1.1167912319891578 11102 62-63 -1.3216533453474604 11102 64-65 -1.3120991838651506 11102 66-67 -0.7291052555416329 11102 68-69 0.6471706967910436 11102 70-71 -1.0451874293086654 11102 72-73 0.22809176129574027 11102 74-75 -3.2317709038423885 11102 76 -1.4928488154269743 11103 1 0.08368447831476189 11103 2 -1.1436941790202013 11103 3 0.8322266392438955 11103 4 -0.5890422461492903 11103 5 -0.2569072029296784 11103 6 0.32451703415303257 11103 7 1.6516143160129069 11103 8 -1.1368943821699666 11103 9 -2.5107687229057483 11103 10-11 0.4389397581099779 11103 12-13 0.849883877442668 11103 14-15 0.08184724132649634 11103 16-17 -0.2891509346844323 11103 18-19 0.3274627331318243 11103 20-21 1.8475207126502013 11103 22-23 -0.1383591750697697 11103 24-25 0.27687866708953734 11103 26-27 0.14189212692449615 11103 28-29 0.3394738117945586 11103 30-31 1.3042321562927235 11103 32-33 1.0137616734544324 11103 34-35 0.9513303832472104 11103 36-37 -0.5457847780964258 11103 38-39 0.5205128486916841 11103 40-41 0.5039180881909964 11103 42-43 0.47351061735082567 11103 44-45 0.3324266136680478 11103 46-47 -0.31937898356900973 11103 48-49 -0.38748229446037286 11103 50-51 -0.8275114981477714 11103 52-53 0.2986160580088395 11103 54-55 0.15518384958241782 11103 56-57 -0.4518020517810122 11103 58-59 0.06783435352969036 11103 60-61 1.4702027458484324 11103 62-63 0.6072379126482765 11103 64-65 0.7870479376700317 11103 66-67 -0.20511804871860306 11103 68-69 0.6542069441257965 11103 70-71 0.1630215259152159 11103 72-73 -0.12308094659338664 11103 74-75 0.16599029018746236 11103 76 -0.5359191779003218 11104 1 -0.04295644832616219 11104 2 -0.13018066550668905 11104 3 0.10751621453346871 11104 4 -0.7068028639099069 11104 5 1.3380734920510164 11104 6 -1.2990350893990907 11104 7 -0.9468412824426906 11104 8 -1.9697129149885022 11104 9 -2.521579533716558 11104 10-11 -1.6826818635116432 11104 12-13 -1.6784945009357095 11104 14-15 -0.4662222567430021 11104 16-17 0.0021618066283046744 11104 18-19 0.8461886018576941 11104 20-21 -1.3094290942996025 11104 22-23 -1.1424132291238287 11104 24-25 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0.9306946403306426 11105 7 -1.2576520932535011 11105 8 0.42217897690339257 11105 9 1.50544749331047 11105 10-11 0.5065073256775463 11105 12-13 1.348532526091315 11105 14-15 1.4391831486624014 11105 16-17 1.2251347796012801 11105 18-19 0.9069994126685081 11105 20-21 1.9540844192139062 11105 22-23 -0.1356564723670708 11105 24-25 0.07765086786173825 11105 26-27 1.1870658720982412 11105 28-29 1.654338676659421 11105 30-31 -0.46854776648720886 11105 32-33 0.6965802062729693 11105 34-35 1.0217937037105322 11105 36-37 0.5113580790464312 11105 38-39 -0.4244678462890086 11105 40-41 1.4475474318203396 11105 42-43 -0.17340057956037214 11105 44-45 1.5696853009267357 11105 46-47 -0.3620430762331033 11105 48-49 0.21792311094502992 11105 50-51 -0.8257740464103165 11105 52-53 -1.5687584593656752 11105 54-55 -0.5591018647032975 11105 56-57 -0.11106846104742019 11105 58-59 0.32053705623238926 11105 60-61 0.8495463751920589 11105 62-63 1.7466201520305162 11105 64-65 -0.045963645341551285 11105 66-67 -0.02751187111242359 11105 68-69 2.3451335850524373 11105 70-71 0.42112963402332326 11105 72-73 0.39892677541433486 11105 74-75 -0.9596853854882141 11105 76 -0.5969230389041833 11106 1 0.7033273354576188 11106 2 -0.06869417902020203 11106 3 1.4250837821010371 11106 4 -0.730113674720716 11106 5 0.5877356542131764 11106 6 -0.6361972515612528 11106 7 0.917685744584336 11106 8 -1.8190372393128236 11106 9 -0.9732687229057468 11106 10-11 -0.1173102418900207 11106 12-13 0.8623838774426709 11106 14-15 -0.9824384729592204 11106 16-17 -0.7748652203987199 11106 18-19 -0.5198586954396056 11106 20-21 1.1939492840787764 11106 22-23 -0.6133591750697711 11106 24-25 -0.4213356186247452 11106 26-27 0.0651064126387837 11106 28-29 0.0269738117945586 11106 30-31 -2.110946415135853 11106 32-33 -2.166595469402708 11106 34-35 0.19865181181864244 11106 36-37 -1.2323919209535674 11106 38-39 -1.1678800084511671 11106 40-41 -1.2451890546661488 11106 42-43 -0.3443465255063174 11106 44-45 -0.3907876720462369 11106 46-47 -0.13991469785472432 11106 48-49 -1.4874822944603743 11106 50-51 -1.107868641004913 11106 52-53 -1.6665625134197306 11106 54-55 -0.1591018647032989 11106 56-57 -1.4607306232095816 11106 58-59 -0.11966564647031319 11106 60-61 -0.5994401112944239 11106 62-63 -0.8266906587802936 11106 64-65 -1.0361663480442544 11106 66-67 -1.1194037630043212 11106 68-69 -0.4949001987313437 11106 70-71 -1.1744784740847862 11106 72-73 -0.6534380894505283 11106 74-75 -0.8027597098125341 11106 76 -1.6644906064717517 11107 1 1.20028679491708 11107 2 0.896846361520339 11107 3 1.7291378361550898 11107 4 -1.1932893503963946 11107 5 -0.13489948092195903 11107 6 -0.8530891434531434 11107 7 0.2288343932329866 11107 8 -0.4697129149885022 11107 9 2.0324745203374945 11107 10-11 0.35110192027213927 11107 12-13 -0.273089095530306 11107 14-15 1.4864804459596996 11107 16-17 1.4413509958174906 11107 18-19 0.5488913045603958 11107 20-21 -0.12023990511041305 11107 22-23 0.38461379790320294 11107 24-25 0.8546778948887699 11107 26-27 -0.7453665603341904 11107 28-29 0.41785219017293684 11107 30-31 -0.23206128000071757 11107 32-33 -0.3236900639973044 11107 34-35 1.9677396496564796 11107 36-37 1.0991959168842698 11107 38-39 1.3390456672245072 11107 40-41 0.43403391830682736 11107 42-43 0.015788609628820893 11107 44-45 0.23184746308889714 11107 46-47 0.9217407075506792 11107 48-49 0.0354906785126019 11107 50-51 0.4782800076437397 11107 52-53 -0.9336233242305418 11107 54-55 0.17738462178319025 11107 56-57 -0.32728467726363775 11107 58-59 -2.415949430254095 11107 60-61 -1.0626157869701025 11107 62-63 -0.6858122804019153 11107 64-65 -0.1473149966928986 11107 66-67 -0.3653497089502622 11107 68-69 -0.12783938792053462 11107 70-71 0.0359944988881864 11107 72-73 -0.08080295431539142 11107 74-75 -0.29752322332605274 11107 76 0.6328066908255465 11108 1 -0.667067401384486 11108 2 -0.14369417902020132 11108 3 1.1224522031536672 11108 4 -1.3202452536680873 11108 5 -0.19713276683945224 11108 6 -1.276986725245461 11108 7 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0.3371751843641029 11108 68-69 0.4231919065318124 11108 70-71 -0.08829426355847048 11108 72-73 0.08669348949684164 11108 74-75 0.22256923755588076 11108 76 0.9012988672124571 11109 1 -1.0391726645423809 11109 2 -1.2103608456868677 11109 3 -1.2649162178989641 11109 4 -0.9842803413873824 11109 5 -0.9664310124534872 11109 6 -0.9678639182279198 11109 7 -2.4664809220823294 11109 8 -2.5073705726461597 11109 9 -0.47743538957241327 11109 10-11 -1.4843935752233541 11109 12-13 -1.9401161225573311 11109 14-15 -1.4757718062925527 11109 16-17 -1.554865220398721 11109 18-19 -1.5377753621062702 11109 20-21 -1.8310507159212221 11109 22-23 -0.25835917506977424 11109 24-25 -2.025502285291413 11109 26-27 -0.7857269206945539 11109 28-29 -1.3838595215387741 11109 30-31 -1.7938630818025239 11109 32-33 0.480904530597293 11109 34-35 -1.8234315215146921 11109 36-37 -1.2753085876202377 11109 38-39 -0.32329667511783455 11109 40-41 -0.3589390546661484 11109 42-43 -2.1993465255063143 11109 44-45 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0.9294144003578495 21611 22-23 0.4058268714418567 21611 24-25 1.3101566294372695 21611 26-27 1.5493118389953686 21611 28-29 0.522031951329442 21611 30-31 0.8886175383525163 21611 32-33 1.3426099569538792 21611 34-35 -0.021260978879034553 21611 36-37 1.069497613930153 21611 38-39 1.2602692163550309 21611 40-41 1.24400668176796 21611 42-43 0.8341418465867108 21611 44-45 1.1005882969460075 21611 46-47 1.060908945556129 21611 48-49 0.5024401861597809 21611 50-51 1.1028871729485772 21611 52-53 1.5884277966577898 21611 54-55 1.0339213911106562 21611 56-57 0.9709554233020476 21611 58-59 1.4201599349250351 21611 60-61 1.041354462348984 21611 62-63 1.150925620289474 21611 64-65 0.1602483806379169 21611 66-67 -0.002737096337650513 21611 68-69 0.38886918111361624 21611 70-71 0.7858897429694771 21611 72-73 0.7278603601618734 21611 74-75 1.1384709103424981 21611 76 0.6417109439158466 21612 1 0.26482733545762116 21612 2 1.088305820979798 21612 3 -0.08091621789896308 21612 4 1.4823863252792826 21612 5 1.1802356542131776 21612 6 0.6988027484387445 21612 7 0.30418574458433767 21612 8 0.8179627606871769 21612 9 0.9892312770942553 21612 10-11 0.4129397581099781 21612 12-13 1.7678838774426673 21612 14-15 1.3615615270407773 21612 16-17 0.46113477960128435 21612 18-19 0.5608913045603963 21612 20-21 1.316949284078781 21612 22-23 0.4776408249302264 21612 24-25 0.865164381375255 21612 26-27 0.6156064126387797 21612 28-29 0.6494738117945573 21612 30-31 1.5408035848641468 21612 32-33 1.5209045305972921 21612 34-35 0.4299018118186382 21612 36-37 0.7993580790464279 21612 38-39 0.5313699915488321 21612 40-41 1.31706094533385 21612 42-43 1.1086534744936856 21612 44-45 0.896712327953761 21612 46-47 0.6983353021452778 21612 48-49 1.2285177055396233 21612 50-51 1.7036313589950893 21612 52-53 -0.008812513419734103 21612 54-55 0.3728981352967047 21612 56-57 1.1787693767904166 21612 58-59 0.46983435352968783 21612 60-61 1.0870598887055714 21612 62-63 0.3778093412197059 21612 64-65 0.07733365195574748 21612 66-67 0.9345962369956808 21612 68-69 0.9673498012686537 21612 70-71 1.2450215259152166 21612 72-73 0.5380619105494695 21612 74-75 1.9399902901874668 21612 76 0.6755093935282481 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 2.0 11 15.0 12 33.0 13 98.0 14 184.0 15 302.0 16 505.0 17 735.0 18 1015.0 19 1427.0 20 2017.0 21 2870.0 22 3912.0 23 4800.0 24 5833.0 25 6328.0 26 6870.0 27 7361.0 28 7937.0 29 8424.0 30 9373.0 31 10439.0 32 11682.0 33 13896.0 34 17082.0 35 17539.0 36 10199.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.33183101578759 15.073768210077015 7.0904969578069705 24.503903816328425 2 36.027784037434216 19.56613952995135 23.175015575498083 21.231060857116347 3 34.791685997958616 18.94709632948475 21.62541921287398 24.635798459682658 4 31.71502803589655 15.887008046236032 21.485571123689336 30.91239279417808 5 32.25387399090656 26.732194223147175 24.81077426795159 16.203157517994672 6 46.93726056814115 25.250202150081524 12.647304444650645 15.165232837126686 7 43.21438513235859 23.544850806611965 12.870663715054548 20.370100345974894 8 41.53090576492266 26.453160831930433 13.925820861888413 18.0901125412585 9 37.42096263206034 15.24741844404088 13.050941820543752 34.28067710335503 10-11 41.48848738716049 20.2908972812471 18.007595540768037 20.213019790824376 12-13 40.13209347949999 19.125386073516353 17.613568578586673 23.12895186839698 14-15 39.65488672967563 18.4397327642201 15.566219064409657 26.339161441694614 16-17 39.745357175996496 20.823446758308037 15.827025808931719 23.604170256763744 18-19 38.96161136812524 21.36726361696205 17.020705470645154 22.650419544267553 20-21 39.70890388260714 20.452948740041624 16.980275454340592 22.857871923010645 22-23 40.00947785628124 20.388326992669576 16.200837762960806 23.40135738808839 24-25 40.190087355346705 20.489402033430984 16.16670422460531 23.153806386617003 26-27 39.85173451397818 20.280955473959093 17.068094752051326 22.799215260011398 28-29 39.89249592385901 20.250135871366272 17.688463526822996 22.168904677951723 30-31 39.92928061082464 20.144089926960856 17.0332984265433 22.893331035671206 32-33 39.629700817879346 20.50696589297313 17.489295987486578 22.374037301660945 34-35 39.608491628998266 20.317408767348454 17.333872400217395 22.74022720343589 36-37 39.534259467914474 19.709964342051194 18.127560015376663 22.62821617465767 38-39 39.00900064953141 19.840202017524092 19.17376953565132 21.97702779729318 40-41 38.89798380148199 19.700022534763185 18.942788212993282 22.459205450761544 42-43 38.41878869020003 19.526040907223056 19.158856824719308 22.896313577857608 44-45 37.33480030223094 19.746749029016822 20.07615424382614 22.842296424926097 46-47 37.5836768780074 19.804411511287263 19.70300507694959 22.90890653375575 48-49 37.40870106973846 19.580058060154563 20.65012791792044 22.361112952186534 50-51 36.69388512573072 19.78121396094858 20.535134346955818 22.989766566364878 52-53 35.934993836079485 21.1243521255584 20.16927583875714 22.77137819960498 54-55 36.35520089078593 20.534802953379554 19.669865719322896 23.44013043651162 56-57 36.09638250772147 20.783679529156007 19.326873367886638 23.793064595235887 58-59 36.3134453001763 21.017312000424184 19.90714351993001 22.762099179469505 60-61 36.26572462519386 20.67697079759806 19.36962313922507 23.687681437983006 62-63 36.54102348270445 20.427898221730285 19.659722820575702 23.37135547498956 64-65 36.54343244210555 20.749214597224245 19.512122377019843 23.195230583650368 66-67 36.81749492967828 20.625936186852954 18.074868436750222 24.48170044671854 68-69 36.89769217513488 20.139781810469387 18.454976868728377 24.507549145667358 70-71 36.9715929426424 19.832579965269954 18.664749002505335 24.531078089582312 72-73 37.69601930036188 19.465727276342477 18.125903047495328 24.712350375800316 74-75 38.229894351727886 18.920584843383395 18.36086109306857 24.488659711820144 76 38.79226925065284 17.696416972653402 18.051008099259004 25.460305677434746 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.5 2 0.5 3 1.0 4 2.0 5 2.5 6 3.0 7 3.0 8 3.0 9 4.5 10 7.0 11 8.5 12 9.0 13 16.0 14 21.5 15 35.5 16 50.0 17 49.0 18 72.5 19 98.5 20 124.0 21 147.0 22 210.0 23 292.0 24 367.0 25 423.0 26 475.5 27 664.0 28 877.5 29 955.0 30 1097.0 31 1389.0 32 1688.0 33 1837.0 34 1971.5 35 2282.5 36 2750.5 37 3042.0 38 3204.0 39 3495.0 40 3748.0 41 3977.0 42 4082.0 43 4060.5 44 4229.5 45 4400.0 46 4380.0 47 4394.5 48 4345.5 49 4053.0 50 3824.0 51 3645.5 52 3415.0 53 3269.5 54 3176.0 55 3155.0 56 3166.5 57 3118.5 58 3038.0 59 3203.5 60 3421.0 61 3501.0 62 3529.0 63 3562.0 64 3590.0 65 3533.0 66 3398.0 67 3315.0 68 3221.5 69 2978.0 70 2757.0 71 2686.0 72 2640.5 73 2536.5 74 2358.0 75 2238.0 76 2180.0 77 1955.5 78 1596.5 79 1404.0 80 1309.0 81 1082.0 82 815.0 83 680.0 84 622.0 85 507.5 86 385.0 87 319.0 88 291.5 89 273.5 90 295.0 91 310.5 92 314.0 93 374.5 94 476.0 95 624.5 96 732.0 97 973.5 98 1661.5 99 10654.5 100 19201.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 6.627871525338352E-4 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 150878.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.32384927992216 #Duplication Level Percentage of deduplicated Percentage of total 1 84.6875775762366 57.861812862005046 2 8.845955876149974 12.08779512037825 3 3.794469316007602 7.777582489325782 4 1.595002402635696 4.3590681503518 5 0.6019971945244135 2.0565382792811002 6 0.22199889633532588 0.9100691480114321 7 0.0869995661165715 0.41609016699370777 8 0.042999785517800114 0.23503286918297236 9 0.02699986532405114 0.16602612560808 >10 0.08699956604398205 0.9831752870350747 >50 0.003999980047999084 0.19267229391514057 >100 0.003999980047999084 0.6012468746288074 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 9.99995011999771E-4 12.35289033328277 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 18080 11.983191717811742 No Hit CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 324 0.21474303742096265 No Hit CTTATACACATCTGACGCTGCCGACGATCCGTGCCGTGTAGATCTCGGTG 298 0.1975105714550829 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.001988361457601506 0.0 2 0.0 0.0 0.0 0.0033139357626691766 0.0 3 0.0 0.0 0.0 0.0033139357626691766 0.0 4 0.0 0.0 0.0 0.005965084372804517 0.0 5 0.0 0.0 0.0 0.01656967881334588 0.0 6 0.0 0.0 0.0 0.01656967881334588 0.0 7 0.0 0.0 0.0 0.01723246596587972 0.0 8 0.0 0.0 0.0 0.01988361457601506 0.0 9 0.0 0.0 0.0 0.020546401728548894 0.0 10 0.0 0.0 0.0 0.023197550338684234 0.0 11 0.0 0.0 0.0 0.023197550338684234 0.0 12 0.0 0.0 0.0 0.024523124643751906 0.0 13 0.0 0.0 0.0 0.024523124643751906 0.0 14 0.0 0.0 0.0 0.02518591179628574 0.0 15 0.0 0.0 0.0 0.026511486101353413 0.0 16 0.0 0.0 0.0 0.026511486101353413 0.0 17 0.0 0.0 0.0 0.026511486101353413 0.0 18 0.0 0.0 0.0 0.02849984755895492 0.0 19 0.0 0.0 0.0 0.029825421864022587 0.0 20 0.0 0.0 0.0 0.03777886769442861 0.0 21 0.0 0.0 0.0 0.041755590609631625 0.0 22 0.0 0.0 0.0 0.05236018505017299 0.0 23 0.0 0.0 0.0 0.05501133366030833 0.0 24 0.0 0.0 0.0 0.06627871525338352 0.0 25 0.0 0.0 0.0 0.07356937393125572 0.0 26 0.0 0.0 0.0 0.0828483940667294 0.0 27 0.0 0.0 0.0 0.14051087633717307 0.0 28 0.0 0.0 0.0 0.18889433847214307 0.0 29 0.0 0.0 0.0 0.2611381380983311 0.0 30 0.0 0.0 0.0 0.31548668460610557 0.0 31 0.0 0.0 0.0 0.39369556860509813 0.0 32 0.0 0.0 0.0 0.46461379392621854 0.0 33 0.0 0.0 0.0 0.5779503970095043 0.0 34 0.0 0.0 0.0 0.7416588236853617 0.0 35 0.0 0.0 0.0 0.9795994114450085 0.0 36 0.0 0.0 0.0 1.4024576147615955 0.0 37 0.0 0.0 0.0 1.9220827423481224 0.0 38 0.0 0.0 0.0 2.4357427855618448 0.0 39 0.0 0.0 0.0 2.941449382945161 0.0 40 0.0 0.0 0.0 3.58236455944538 0.0 41 0.0 0.0 0.0 4.2219541616405305 0.0 42 0.0 0.0 0.0 4.8714855711236895 0.0 43 0.0 0.0 0.0 5.552830763928472 0.0 44 0.0 0.0 0.0 6.289850077546097 0.0 45 0.0 0.0 0.0 7.083206299129098 0.0 46 0.0 0.0 0.0 7.8242023356619255 0.0 47 0.0 0.0 0.0 8.541338034703536 0.0 48 0.0 0.0 0.0 9.12459072893331 0.0 49 0.0 0.0 0.0 9.657471599570513 0.0 50 0.0 0.0 0.0 10.166492132716499 0.0 51 0.0 0.0 0.0 10.668222007184612 0.0 52 0.0 0.0 0.0 11.283951271888546 0.0 53 0.0 0.0 0.0 11.897029387982343 0.0 54 0.0 0.0 0.0 12.42725911000941 0.0 55 0.0 0.0 0.0 12.982011956680232 0.0 56 0.0 0.0 0.0 13.519532337385172 0.0 57 0.0 0.0 0.0 14.053738782327443 0.0 58 0.0 0.0 0.0 14.54221291374488 0.0 59 0.0 0.0 0.0 15.030024258009783 0.0 60 0.0 0.0 0.0 15.460173120004242 0.0 61 0.0 0.0 0.0 15.896287066371505 0.0 62 0.0 0.0 0.0 16.424528426940974 0.0 63 0.0 0.0 0.0 16.985246357984597 0.0 64 0.0 0.0 0.0 17.484325083842574 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCTTAAC 20 0.0069403937 52.5 1 TAAGTAC 20 0.0069403937 52.5 28 TCGGCTA 20 0.0069403937 52.5 15 GTAATAG 20 0.0069403937 52.5 1 CTCTAGT 20 0.0069403937 52.5 4 TTCCTAT 20 0.0069403937 52.5 36 ACTGGAC 20 0.0069403937 52.5 65 CGTATCA 75 0.0 51.33333 56 CCGTATC 65 1.8371793E-10 48.461536 55 GCCGTAT 65 1.8371793E-10 48.461536 54 GTATCAT 75 7.4760464E-10 41.999996 57 CAGCGGA 35 0.0012523044 40.0 9 TCCATGG 35 0.0012523044 40.0 2 CAGACAT 35 0.0012523044 40.0 5 GTAGATC 90 1.03682396E-10 38.888885 38 CGGTGGT 95 1.8553692E-10 36.842106 46 GTGGTCG 130 0.0 35.0 48 TGGTCGC 110 2.3646862E-11 35.0 49 CGCCGTA 95 7.550625E-9 33.157898 53 TCGCCGT 120 6.366463E-11 32.083336 52 >>END_MODULE