FastQCFastQC Report
Wed 25 May 2016
SRR1781229_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781229_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150878
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT61424.070838690862817No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG22501.4912710932011293No Hit
CTTAAACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT16711.1075173318840388No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC7510.49775315155291033No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7160.4745556012142261No Hit
CTAATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT4690.31084717453836874No Hit
CTAAAACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCGT3230.2140802502684288No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCC3110.20612680443802275No Hit
TCATATACACATCTCCGAGCCCACGAGACGAGCACGTATCTCGTATGCCG3050.20215008152281977No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTAAT150.00221852170.0208947
TCGAAAG150.002221441169.9976840
CGAATGC501.4551915E-1162.99791341
AGGGGGG7550.059.3357970
CGAAACT308.346638E-658.331434
CGTAATC252.3808461E-456.0167148
CTTTATA653.6379788E-1253.8443682
TCGAATG653.6379788E-1253.84436840
CTTAAAC3950.053.1627961
AACACAT6700.052.7594455
TTGGACC200.00693220552.51566746
CCGTCTA608.367351E-1152.51566747
TAAGCCG800.052.4982643
TCGTAAG950.051.5772440
AACGTAT750.051.33163532
ACGTAAC750.051.33163533
TTAAACA4400.050.9074062
TAAACAC4700.049.891963
AAAACAC3100.049.675773
AAACACA7500.049.4650274