FastQCFastQC Report
Wed 25 May 2016
SRR1781228_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781228_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences253553
Sequences flagged as poor quality0
Sequence length76
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3232212.747630672877072No Hit
CTTATACACATCTGACGCTGCCGACGATCCGTGCCGTGTAGATCTCGGTG6010.23703131100795494No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4870.19207029693989028No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3000.11831845807385437No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAC150.002223073369.9986313
CGGGATT252.3877044E-455.99889826
CGTATCA1350.051.8508356
GCCGTAT1400.047.49906554
GTATAAC305.87126E-446.665751
CCGTATC1550.045.16040455
CGGTGGT1850.041.620846
CGCCGTA1750.039.9992153
TAGATCT1550.038.3863439
GTAGATC1650.036.05989538
TGTAGAT2150.035.81324837
TCGGTGG2150.034.18537545
AGATCTC1750.033.9993340
GTATCAT1650.033.93872557
ATCTCGG1900.033.1572442
TCTCGGT1800.033.054943
TATCATT1600.032.8118558
CGTGTAG2300.031.95589335
ATCATTA1650.031.81755459
ACGAGTC450.00429845531.1166346
Produced by FastQC (version 0.11.5)