FastQCFastQC Report
Wed 25 May 2016
SRR1781228_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781228_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences253553
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT129075.090454461197462No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG34911.3768324571194188No Hit
CTTAAACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT31591.2458933635176865No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC13470.5312498767516062No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12560.49535994446920373No Hit
CTAATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT8750.3450955027154086No Hit
CTAAAACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCGT5950.23466494184647785No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCC4930.19443666610136737No Hit
TCATATACACATCTCCGAGCCCACGAGACAATGGTAGATCTCGTATGCCG3780.14908125717305654No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGGAAGATCTCGTATGCCGT2660.10490903282548422No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAA252.8158156E-670.0783946
ACTATCG207.927002E-569.9816727
TACGATA150.002225210769.9816610
AGGGGGG13850.059.87600370
CTTAAAC5700.056.4764251
GTAGACT252.3905652E-455.985333
ACTCGTA1250.055.9853338
CCGTAAT452.6686394E-854.5054247
AACACAT9650.054.3898935
TTAAACA6500.054.370372
AAACACA10650.053.225494
TAAGCCG608.367351E-1152.5484243
CGTAAGC800.052.53804841
ACGTATG608.367351E-1152.49659740
ATTCGAG200.006953020652.4862485
CGTAGAA200.006953020652.48624832
GTAAACG200.006953020652.48624825
TAAACAC7350.052.3672263
AACTCGT1300.051.1404537
CCGTCTT30550.049.7774547