FastQCFastQC Report
Wed 25 May 2016
SRR1781227_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781227_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143940
Sequences flagged as poor quality0
Sequence length76
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2699618.755036820897597No Hit
CTTATACACATCTGACGCTGCCGACGATCCGTGCCGTGTAGATCTCGGTG5770.4008614700569682No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3860.2681672919271919No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2140.14867305821870225No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1510.10490482145338335No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTATA150.002220940370.000015
GTATCAT1400.052.50000457
CGAACCT200.006939747452.553
TCTTATC200.006939747452.570
ATCCAAT200.006939747452.543
ATAACTA200.006939747452.57
CACGTTT200.006939747452.513
TAGATCT1300.051.15384739
CGCCGTA1300.051.15384753
CGTATCA1450.050.68965556
GCCGTAT1250.050.39999854
ATCATTA1300.045.7692359
ATCTCGG1400.045.00000442
GTAGATC1600.043.7538
TGTAGAT1600.043.7537
AGATCTC1550.042.9032340
TCATTAA1400.042.50000460
CGGTGGT1700.041.1764746
CCGTATC1650.040.30303255
TACACCC350.001252111740.00000433