FastQCFastQC Report
Wed 25 May 2016
SRR1781227_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781227_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143940
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT100316.968875920522439No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG29632.058496595803807No Hit
CTTAAACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT24991.7361400583576492No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11780.8183965541197722No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC10830.752396832013339No Hit
CTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT7430.5161873002639988No Hit
CTAAAACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGT4900.34041961928581355No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC4400.30568292344032233No Hit
TCATATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCG3490.2424621370015284No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACAGATCTCGTATGCCGT2370.16465193830762817No Hit
ATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT1840.12783104071140752Illumina PCR Primer Index 9 (95% over 22bp)
ATACCCATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCCGTCTT1800.12505210504376824Illumina PCR Primer Index 9 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGTTAAAAGATCTCGTATGCCGT1730.12018896762539946No Hit
CTCTAATACACATCTCCGAGCCCACGAGACGTTAACAGATCTCGTATGCC1730.12018896762539946No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAAACAGATCTCGTATGCCGT1460.10143115186883424No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAA150.002208929370.0957246
CTTAAGG150.002224222569.973942
GGTTGTA150.002224222569.9739414
TAAGGCA150.002224222569.973944
ATAACCG150.002224222569.9739410
ACGAATG150.002224222569.9739440
CTTTATA800.061.2271962
TCTTTAT750.060.6440851
TTAATAC308.361483E-658.311623
CTCGAAA308.361483E-658.3116239
TCGAAAG308.361483E-658.3116240
AGGGGGG12750.056.25356370
ACGTCTA252.367935E-456.07657647
CCGACTT750.056.07657247
TATCTCG252.3883797E-455.97915336
TTTATAC950.055.242583
CTTAAAC5900.055.1489521
TTAAACA6350.055.097592
AACACAT9150.054.679095
AAACACA10050.053.9600034
Produced by FastQC (version 0.11.5)