FastQCFastQC Report
Wed 25 May 2016
SRR1781226_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781226_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171644
Sequences flagged as poor quality0
Sequence length76
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3511420.457458460534596No Hit
CTTATACACATCTGACGCTGCCGACGATCCGTGCCGTGTAGATCTCGGTG8210.4783155834168395No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4330.2522663186595512No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2350.13691128148959475No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1970.11477243597212836No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGCCC200.006942012352.50000418
CTCCCAT200.006942012352.5000041
GCAGTCG200.006942012352.50000454
ATTTTAG200.006942012352.50000468
CTGCAAT200.006942012352.50000413
GGATTGA200.006942012352.50000410
CAATCTA200.006942012352.50000410
CTAGTGT305.865355E-446.66666445
GGATTAA305.865355E-446.6666641
CGTATCA1150.042.60869656
GCCGTAT1150.042.60869654
TTGTGCG350.001252786340.02
CGGTTGA350.001252786340.024
GTATCAT1200.037.91666457
CTTGTGC400.002413721335.0000041
TTCTAGC400.002413721335.0000042
TGTGGCG400.002413721335.00000411
TGTATCT400.002413721335.00000437
CCGTATC1400.035.055
CATTAAA1550.033.87096861
Produced by FastQC (version 0.11.5)