Basic Statistics
Measure | Value |
---|---|
Filename | SRR1781226_1.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 171644 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 54 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT | 13706 | 7.985132017431427 | No Hit |
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG | 4045 | 2.3566218452145136 | No Hit |
CTTAAACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT | 3461 | 2.016382745682925 | No Hit |
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC | 1526 | 0.8890494278856237 | No Hit |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 1459 | 0.8500151476311436 | No Hit |
CTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT | 920 | 0.5359931020018177 | No Hit |
CTAAAACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT | 696 | 0.4054904336883317 | No Hit |
TCTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC | 515 | 0.3000396168814523 | No Hit |
TCATATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG | 469 | 0.2732399617813614 | No Hit |
ATACCCATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT | 256 | 0.14914590664398406 | RNA PCR Primer, Index 42 (95% over 22bp) |
CTCTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC | 206 | 0.12001584675258092 | No Hit |
CTTATACACATCTCCGAGCCCACGAGACATAACTCGATCTCGTATGCCGT | 187 | 0.10894642399384774 | No Hit |
CTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC | 179 | 0.10428561441122323 | RNA PCR Primer, Index 32 (95% over 21bp) |
ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT | 176 | 0.10253781081773904 | RNA PCR Primer, Index 42 (95% over 22bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CTCGCTT | 15 | 0.0022161708 | 70.04375 | 45 |
TGCCGTA | 15 | 0.0022161708 | 70.04375 | 45 |
CGTATAC | 15 | 0.002218736 | 70.02332 | 41 |
TTGAGCG | 15 | 0.0022238733 | 69.98252 | 16 |
CGAAAAT | 20 | 7.9192316E-5 | 69.98251 | 26 |
CTAGATC | 45 | 3.8380676E-10 | 62.20668 | 33 |
ACTAGAT | 40 | 1.0621079E-8 | 61.234703 | 32 |
AACTCGT | 115 | 0.0 | 60.872097 | 37 |
AACACAT | 970 | 0.0 | 60.242687 | 5 |
AGGGGGG | 1435 | 0.0 | 59.985016 | 70 |
CTCGTAA | 95 | 0.0 | 58.949825 | 39 |
CTTAAAC | 625 | 0.0 | 58.802444 | 1 |
AAACACA | 1025 | 0.0 | 58.375664 | 4 |
ACTCGTA | 120 | 0.0 | 58.335762 | 38 |
ATCGATC | 30 | 8.362247E-6 | 58.318768 | 33 |
TTAAACA | 670 | 0.0 | 57.970592 | 2 |
TAAACAC | 715 | 0.0 | 56.76904 | 3 |
TCGTAAG | 105 | 0.0 | 56.66902 | 40 |
GTAAGAC | 25 | 2.3813633E-4 | 56.018654 | 42 |
TAAGACG | 25 | 2.3813633E-4 | 56.018654 | 43 |