FastQCFastQC Report
Wed 25 May 2016
SRR1781226_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781226_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences171644
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT137067.985132017431427No Hit
TCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG40452.3566218452145136No Hit
CTTAAACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT34612.016382745682925No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC15260.8890494278856237No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14590.8500151476311436No Hit
CTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT9200.5359931020018177No Hit
CTAAAACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGT6960.4054904336883317No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC5150.3000396168814523No Hit
TCATATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCG4690.2732399617813614No Hit
ATACCCATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT2560.14914590664398406RNA PCR Primer, Index 42 (95% over 22bp)
CTCTAATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCC2060.12001584675258092No Hit
CTTATACACATCTCCGAGCCCACGAGACATAACTCGATCTCGTATGCCGT1870.10894642399384774No Hit
CTATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTC1790.10428561441122323RNA PCR Primer, Index 32 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACATCACTCGATCTCGTATGCCGTCTT1760.10253781081773904RNA PCR Primer, Index 42 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCTT150.002216170870.0437545
TGCCGTA150.002216170870.0437545
CGTATAC150.00221873670.0233241
TTGAGCG150.002223873369.9825216
CGAAAAT207.9192316E-569.9825126
CTAGATC453.8380676E-1062.2066833
ACTAGAT401.0621079E-861.23470332
AACTCGT1150.060.87209737
AACACAT9700.060.2426875
AGGGGGG14350.059.98501670
CTCGTAA950.058.94982539
CTTAAAC6250.058.8024441
AAACACA10250.058.3756644
ACTCGTA1200.058.33576238
ATCGATC308.362247E-658.31876833
TTAAACA6700.057.9705922
TAAACAC7150.056.769043
TCGTAAG1050.056.6690240
GTAAGAC252.3813633E-456.01865442
TAAGACG252.3813633E-456.01865443