FastQCFastQC Report
Wed 25 May 2016
SRR1781225_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781225_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169889
Sequences flagged as poor quality0
Sequence length76
%GC82

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6500938.26557340381072No Hit
CTTATACACATCTGACGCTGCCGACGATCCGTGCCGTGTAGATCTCGGTG14420.8487895037347916No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7040.41438821818952376No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6040.35552625537851185No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3330.19601033616066962No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2280.13420527520910713No Hit
GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2130.12537598078745535No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2110.12419874153123511No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2010.1183125452501339No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACCG150.002221891769.997945
AATCATT150.002221891769.9979458
TCTCTAG150.002221891769.9979454
TAGCGCC150.002221891769.9979458
TTGTACG150.002221891769.997942
ATATCGG301.0192343E-769.9979442
GATATCG353.6889105E-969.9979441
TAGATAT401.3460522E-1069.9979439
TGTAGGT150.002221891769.9979437
CCGAATC301.0192343E-769.9979455
AACTCAC150.002221891769.9979410
CGTCAGT150.002221891769.9979415
AGTGTGG150.002221891769.9979435
TGGTATG150.002221891769.9979440
TTCTCTA150.002221891769.9979453
GTAGATA453.8198777E-1062.22038738
AGATATC401.060107E-861.24819640
CGAATCA401.060107E-861.24819656
ATCATTA2800.061.24819659
TATCATT2700.060.9241358
Produced by FastQC (version 0.11.5)