FastQCFastQC Report
Wed 25 May 2016
SRR1781225_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781225_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169889
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT2664615.68435861062223No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG70444.146236660407678No Hit
CTTAAACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT67523.9743597289995236No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28761.692870050444702No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC25951.5274679349457587No Hit
CTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT17631.0377364043581396No Hit
CTAAAACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT12840.7557876024933927No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC9080.534466622323988No Hit
TCATATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCG7040.41438821818952376No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGATAATCTCGTATGCCGT6730.39614100971811No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTAAATCTCGTATGCCGT5020.2954870533112797No Hit
CTCTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC3820.22485269793806545No Hit
ATACCCATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT3520.20719410909476188RNA PCR Primer, Index 15 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCAGT3360.19777619504499996No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGACGT3330.19601033616066962No Hit
CTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC3190.18776966136712794No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAGTTAATATCGTATGCCGT3140.18482656322657737No Hit
CTTATACACATCTCCGCGCCCACGAGACATAAGTTAATCTCGTATGCCGT3040.17894036694547616No Hit
ATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTCTT2730.16069315847406246RNA PCR Primer, Index 15 (95% over 21bp)
ATTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT2700.15892729958973212No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCC2380.1400914714902083No Hit
CTTAAACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGACGT2260.13302803595288687No Hit
CTTATACACATCTCCGAGCCCACGAGACAAAAGTTAATCTCGTATGCCGT2230.13126217706855653No Hit
CTTAAACACATCTCCGAGCCCACGAGACATAAGATAATCTCGTATGCCGT2110.12419874153123511No Hit
CTTAAACACATCTCCGAGCCCACGAGACATAAGTTAATATCGTATGCCGT2100.12361012190312498No Hit
CTTAAACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCAGT1970.11595806673769345No Hit
TCTTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCAG1920.11301496859714283No Hit
TTATACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGTC1760.10359705454738094No Hit
ATTAAACACATCTCCGAGCCCACGAGACATAAGTTAATCTCGTATGCCGT1740.1024198152911607No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAT150.002217223970.0350545
GTATCCA150.002217223970.0350542
CCGAATA207.889611E-570.0350547
GCAGACT252.8221657E-670.0350546
ACGACTT207.889611E-570.0350547
TATCCAG150.002217223970.0350543
GAATACT207.901134E-570.0144349
ATTCATC150.002219816270.0144351
AATGAAT150.002219816270.0144353
CGAATAC150.002219816270.0144348
AATACTG150.002219816270.0144350
AGACTTT150.002222410669.9938225
TGATAGA150.002222410669.9938255
AAATCGA150.002222410669.9938237
TCATTAT150.002222410669.993821
GGTTTGC150.002222410669.9938222
TAACGTA207.9126694E-569.9938238
ATAGAAA301.0196527E-769.9938257
AAAACTC150.002222410669.9938235
CATTATA150.002222410669.993822
Produced by FastQC (version 0.11.5)