FastQCFastQC Report
Wed 25 May 2016
SRR1781224_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781224_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118865
Sequences flagged as poor quality0
Sequence length76
%GC79

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3940133.14768855424221No Hit
CTTATACACATCTGACGCTGCCGACGATCCGTGCCGTGTAGATCTCGGTG6990.588062087241829No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5490.4618685062886468No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2790.23472006057291883No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2150.18087746603289448No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1690.1421781012072519No Hit
GGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1430.12030454717536702No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1350.11357422285786396No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAAT150.002219986470.058
ATCAATA207.89945E-570.059
AATCATT252.3822946E-455.99999658
TAGGTGG252.3822946E-455.99999645
GCCGTAT1200.055.41666454
AAAAAGG700.055.00000470
CGTATCA1100.054.09090856
TCATTAA1250.053.19999760
ATTAAAA1400.052.50000462
GTCGCGT200.006936786752.553
TCTTGGT200.006936786752.58
GTCTGAC200.006936786752.550
AACCACT200.006936786752.55
CCATGTT200.006936786752.514
GCCGAAT200.006936786752.554
CCATCTG200.006936786752.568
ATCCAGT200.006936786752.51
GAACGCT200.006936786752.518
GTATCAA200.006936786752.557
ATCATTA1200.052.559
Produced by FastQC (version 0.11.5)