FastQCFastQC Report
Wed 25 May 2016
SRR1781224_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781224_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118865
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT1633713.744163546880916No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG44443.738695158372944No Hit
CTTAAACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT37313.1388550035754843No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC15781.3275564716274766No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15211.2796029108652673No Hit
CTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT10830.9111176544819753No Hit
CTAAAACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT6990.588062087241829No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC5680.4778530265427165No Hit
TCATATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCG4590.38615235771673745No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCTAAGATCTCGTATGCCGT2600.2187355403188491No Hit
ATACCCATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT2480.20864005384259454Illumina PCR Primer Index 9 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCTT2290.1926555335885248Illumina PCR Primer Index 9 (95% over 21bp)
CTCTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC2220.18676649981070964No Hit
CTTATACACATCTCCGCGCCCACGAGACTTGCTAAGATCTCGTATGCCGT2200.18508391873133387No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGACGT2170.18256004711227022No Hit
ATTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGT1960.1648929457788247No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCAGT1750.1472258444453792No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCC1700.1430193917469398No Hit
CTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTC1700.1430193917469398No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTAAGCTCTCGTATGCCGT1660.13965422958818827No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCAAAGATCTCGTATGCCGT1600.13460648635006098No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGCTCTCGTATGCCG1370.11525680393723974No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGATAAGATCTCGTATGCCGT1330.11189164177848819No Hit
TATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCCGTCT1320.11105035123880032No Hit
TCTTATACACATCTCCGCGCCCACGAGACTTGCTAAGATCTCGTATGCCG1310.11020906069911245No Hit
CTTAAACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGACGT1310.11020906069911245No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTGCTAAGATCTCGTATGCAG1200.10095486476254575No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCAA150.00221400770.04758553
ACTGCAA150.00221400770.04758553
CCGTATT301.0116128E-770.04758547
TTAAGCT150.00221400770.04758552
ATTCTGC150.00221400770.04758551
ACATCAG252.8139475E-670.0475850
TAAGCTT207.872885E-570.0475853
GCAGACA207.872885E-570.0475846
AAAGCCG252.8139475E-670.0475843
ACGACTT207.872885E-570.0475847
CGAATAA150.002217708670.018141
TGATAGA150.00222141569.9886555
GCTATCG150.00222141569.9886536
ATTCGCC150.00222141569.9886520
GGTCAGA150.00222141569.988651
TGAGTAA150.00222141569.9886510
ATCGTAA301.0175427E-769.9886539
ACGATAC150.00222141569.9886523
ACTAGAT301.0175427E-769.9886527
TAGATGC150.00222141569.9886536
Produced by FastQC (version 0.11.5)