FastQCFastQC Report
Wed 25 May 2016
SRR1781223_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781223_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104557
Sequences flagged as poor quality0
Sequence length76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1539214.721156880935757No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2570.24579894220377402No Hit
CTTATACACATCTGACGCTGCCGACGATCCGTGCCGTGTAGATCTCGGTG2220.2123243780904196No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1090.10424935681016097No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTAC150.002219236570.012
GATACGT150.002219236570.026
ATACGTG150.002219236570.027
CGATACG150.002219236570.025
GGATATT200.00693446252.51
TGTGATA200.00693446252.543
AGACACG200.00693446252.57
TTCGCTC200.00693446252.554
CCGTTCG200.00693446252.51
AGTGTTA200.00693446252.511
AGTAACT200.00693446252.551
GCCGTAT707.2759576E-1250.054
CCGTATC800.048.12555
CGCCGTA800.048.12553
CGTATCA851.8189894E-1245.29411756
TATCATT703.783498E-1045.058
ATCTCGG1050.043.33333642
CGTGTAG1050.043.33333635
TCTCGGT1000.042.043
GTATCAT953.6379788E-1240.52631857
Produced by FastQC (version 0.11.5)