##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1781223_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 104557 Sequences flagged as poor quality 0 Sequence length 76 %GC 65 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.56787206978012 32.0 27.0 32.0 13.0 32.0 2 28.126706007249634 32.0 27.0 32.0 13.0 32.0 3 27.76493204663485 32.0 27.0 32.0 13.0 32.0 4 26.631272894210813 32.0 27.0 32.0 13.0 32.0 5 27.564763717398165 32.0 27.0 32.0 13.0 32.0 6 31.317530151018104 37.0 32.0 37.0 13.0 37.0 7 30.93395946708494 37.0 27.0 37.0 13.0 37.0 8 31.383809787962548 37.0 32.0 37.0 13.0 37.0 9 31.26304312480274 37.0 27.0 37.0 13.0 37.0 10-11 30.925193913367828 37.0 27.0 37.0 10.5 37.0 12-13 30.742647551096532 37.0 27.0 37.0 13.0 37.0 14-15 31.64250121943055 37.0 32.0 37.0 13.0 37.0 16-17 31.78158803332154 37.0 29.5 37.0 13.0 37.0 18-19 31.040523350899512 37.0 27.0 37.0 13.0 37.0 20-21 31.46524383828916 37.0 29.5 37.0 13.0 37.0 22-23 31.38679858832981 37.0 29.5 37.0 13.0 37.0 24-25 30.7017464158306 37.0 27.0 37.0 13.0 37.0 26-27 31.231452700440908 37.0 29.5 37.0 13.0 37.0 28-29 30.70812571133449 37.0 27.0 37.0 13.0 37.0 30-31 30.917298698317666 37.0 27.0 37.0 13.0 37.0 32-33 30.91103417274788 37.0 27.0 37.0 13.0 37.0 34-35 30.84446761096818 37.0 29.5 37.0 13.0 37.0 36-37 31.572405482177185 37.0 29.5 37.0 13.0 37.0 38-39 31.35660931358015 37.0 29.5 37.0 13.0 37.0 40-41 30.59661237411173 37.0 29.5 37.0 10.5 37.0 42-43 30.682737645494804 37.0 27.0 37.0 13.0 37.0 44-45 30.34680126629494 37.0 27.0 37.0 13.0 37.0 46-47 30.73132836634563 37.0 29.5 37.0 13.0 37.0 48-49 31.170304235966984 37.0 29.5 37.0 13.0 37.0 50-51 31.370486911445433 37.0 32.0 37.0 13.0 37.0 52-53 30.38885488298249 37.0 27.0 37.0 13.0 37.0 54-55 30.561908815287353 37.0 27.0 37.0 13.0 37.0 56-57 30.282176229233816 37.0 27.0 37.0 10.5 37.0 58-59 29.86938225082969 37.0 24.5 37.0 10.5 37.0 60-61 29.597827022580987 37.0 22.0 37.0 8.0 37.0 62-63 29.325148005394187 37.0 22.0 37.0 8.0 37.0 64-65 29.16841053205429 37.0 22.0 37.0 8.0 37.0 66-67 29.31433572118557 37.0 22.0 37.0 8.0 37.0 68-69 29.686333770096695 37.0 22.0 37.0 8.0 37.0 70-71 29.791563453427315 37.0 22.0 37.0 8.0 37.0 72-73 30.115123808066414 37.0 27.0 37.0 13.0 37.0 74-75 30.45194965425557 37.0 27.0 37.0 13.0 37.0 76 30.2175464100921 37.0 27.0 37.0 13.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 11101 1 -0.05788368812240563 11101 2 0.9186800275032532 11101 3 1.136851311458333 11101 4 0.9766342213902384 11101 5 1.6013898254338343 11101 6 0.9153325479775489 11101 7 0.5130425677487764 11101 8 -0.7895088932620524 11101 9 0.4670815799456456 11101 10-11 0.3571218647797245 11101 12-13 1.1231717098213494 11101 14-15 0.8947471394211597 11101 16-17 0.7133398795594132 11101 18-19 0.281476411674376 11101 20-21 -0.6580311622982542 11101 22-23 1.275012566505513 11101 24-25 0.5714381618113329 11101 26-27 -1.3240265750043214 11101 28-29 -0.3980357737722322 11101 30-31 0.2525243053741235 11101 32-33 -0.8692025328841488 11101 34-35 -0.7243770641760641 11101 36-37 0.5055729737030425 11101 38-39 -0.6018737058337003 11101 40-41 0.6925135999479508 11101 42-43 1.6537438536928768 11101 44-45 0.8212175146019547 11101 46-47 2.1703266688659966 11101 48-49 0.7515362618427375 11101 50-51 0.08002563329009149 11101 52-53 -1.2153936267307515 11101 54-55 1.7247508831878484 11101 56-57 0.4136020733579393 11101 58-59 2.2978753038197617 11101 60-61 -1.1761198550438863 11101 62-63 -0.7301067067007629 11101 64-65 1.3938834425494342 11101 66-67 1.685464181346088 11101 68-69 0.36319854495639703 11101 70-71 0.46156735956998673 11101 72-73 -0.08979837772283972 11101 74-75 -0.6513920472731023 11101 76 -1.3030522722504614 11102 1 -1.068769388270848 11102 2 -0.9091279883304928 11102 3 -0.7661669963100977 11102 4 -0.2796745366503366 11102 5 -1.8419550533390492 11102 6 0.7352236909760634 11102 7 1.206758549935813 11102 8 -2.3070744548652193 11102 9 -1.750632423023184 11102 10-11 -2.9845406785156747 11102 12-13 -1.0495150788971088 11102 14-15 0.3341335817764275 11102 16-17 0.9416921804005796 11102 18-19 -0.48596544879074344 11102 20-21 -2.1102825230107705 11102 22-23 1.0241466585391628 11102 24-25 -0.7568646585746137 11102 26-27 -0.6807806571418773 11102 28-29 -0.3473183071715411 11102 30-31 -0.2826068178322103 11102 32-33 -0.11784182036559443 11102 34-35 -0.3337288702126777 11102 36-37 -0.7148624543028994 11102 38-39 0.026280673186587222 11102 40-41 -0.26839684042809964 11102 42-43 -2.4734209162230094 11102 44-45 -0.49323077832233864 11102 46-47 -1.3873180614655247 11102 48-49 -0.7831000568113957 11102 50-51 -1.9951846586445896 11102 52-53 -2.3616083719064065 11102 54-55 -1.9400190326953783 11102 56-57 -0.13983681827986416 11102 58-59 -0.15882929786257804 11102 60-61 -0.21941525336154655 11102 62-63 -0.45450057112430997 11102 64-65 -2.9465420893654617 11102 66-67 -1.8501617464124465 11102 68-69 -1.9547133798333114 11102 70-71 0.3376188192434171 11102 72-73 -2.4203278185936945 11102 74-75 -1.3114613199104106 11102 76 -1.6548583088660003 11103 1 1.2665902875143615 11103 2 1.180031079551977 11103 3 0.49067190946497874 11103 4 -1.8683270189198389 11103 5 1.77303988080482 11103 6 1.7276249067815357 11103 7 -0.19293749869640564 11103 8 -0.013761384956403333 11103 9 0.3375134736333507 11103 10-11 -0.49669873322692126 11103 12-13 -0.6439932957157488 11103 14-15 -0.2757954962377589 11103 16-17 1.0810032239669454 11103 18-19 2.0735583607330668 11103 20-21 0.8137296350439378 11103 22-23 0.7678143383770539 11103 24-25 0.7674514508478794 11103 26-27 0.4722081979746413 11103 28-29 1.727656363547812 11103 30-31 -0.3150282970621987 11103 32-33 2.227142982066013 11103 34-35 -0.068784594630106 11103 36-37 0.004742408918989582 11103 38-39 2.1420354857499113 11103 40-41 1.0208635445769652 11103 42-43 -0.30472790710446773 11103 44-45 1.4062119775033963 11103 46-47 0.546572516042076 11103 48-49 1.7136071367043115 11103 50-51 0.19630470305753533 11103 52-53 0.798725974598149 11103 54-55 0.7887043715599411 11103 56-57 0.20236176328041822 11103 58-59 -0.9136418611857771 11103 60-61 -0.8574598328954899 11103 62-63 0.48445586251297 11103 64-65 1.0605501092160985 11103 66-67 -1.3402833269595575 11103 68-69 0.4313048572487581 11103 70-71 -0.679351798791032 11103 72-73 2.562194977758889 11103 74-75 -0.8714917150471884 11103 76 1.741798226088406 11104 1 0.058395381645038213 11104 2 -2.895273460868843 11104 3 -1.1189626420300378 11104 4 0.1859365469716323 11104 5 -0.9800055234033742 11104 6 -2.642806986906173 11104 7 -1.7660271996930845 11104 8 -0.2313693583783305 11104 9 -1.742220745635752 11104 10-11 -2.1545060421970206 11104 12-13 -1.4000841041321408 11104 14-15 -1.1633923954625622 11104 16-17 -4.344799655324309 11104 18-19 0.7814764116743724 11104 20-21 -0.3091939529959298 11104 22-23 -1.0156851079130895 11104 24-25 0.303996301346217 11104 26-27 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68-69 -0.938443041910908 11105 70-71 -1.8061024990711445 11105 72-73 -2.7970487197201024 11105 74-75 -2.1817158046830407 11105 76 0.8328346406542337 11106 1 1.6407399473783073 11106 2 -0.5478600769106059 11106 3 -1.2134097231880823 11106 4 -0.22887123660691344 11106 5 0.16759770393407436 11106 6 -0.6499261136266306 11106 7 1.2662461747729807 11106 8 -0.5137613849564033 11106 9 -1.5196293835095034 11106 10-11 -0.15996403934936865 11106 12-13 -0.8497756086409183 11106 14-15 -0.044502979230955475 11106 16-17 0.8633161491370132 11106 18-19 -0.21045524470910948 11106 20-21 0.32393371667658855 11106 22-23 -0.6777638929154612 11106 24-25 0.14500247125604204 11106 26-27 -0.07200948910019278 11106 28-29 -0.47302390856103216 11106 30-31 0.24279483218950304 11106 32-33 -0.01775497711765439 11106 34-35 -1.1878322136777264 11106 36-37 1.999640368102657 11106 38-39 0.6845524925526298 11106 40-41 0.14671388471301938 11106 42-43 -1.5292177030228338 11106 44-45 0.9453276237618979 11106 46-47 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21611 40-41 -0.6250657949007632 21611 42-43 0.08697716201995931 21611 44-45 0.23532104048342717 21611 46-47 0.5884311949975292 21611 48-49 -0.26834370814976083 21611 50-51 -0.2445555119962286 21611 52-53 -0.948201828781265 21611 54-55 0.10859684467822106 21611 56-57 0.860963913818054 21611 58-59 -0.7216295723992978 21611 60-61 -0.6351096024807461 21611 62-63 -0.7524873018495448 21611 64-65 -0.8588047294935741 21611 66-67 -0.07722649132207948 21611 68-69 -0.37898700143020037 21611 70-71 -0.9585376666865777 21611 72-73 -0.6509386627941076 21611 74-75 0.737955289561107 21611 76 2.297097765258915 21612 1 1.5163369947078777 21612 2 1.1547017989035488 21612 3 0.20191811007288152 21612 4 0.3160701442007259 21612 5 2.083576861554569 21612 6 0.5118194356569141 21612 7 0.3982479116379416 21612 8 0.6610106515177598 21612 9 0.9727718322959653 21612 10-11 1.3612040023353913 21612 12-13 2.0781444955709745 21612 14-15 0.908601666882813 21612 16-17 1.1970113293367461 21612 18-19 0.9076515724858538 21612 20-21 1.0173770514573128 21612 22-23 1.4443594244966071 21612 24-25 1.2484544903615529 21612 26-27 1.7926235981772756 21612 28-29 0.9026816928284624 21612 30-31 1.669520841742262 21612 32-33 1.206626264740791 21612 34-35 0.8790370872341278 21612 36-37 0.7266269073992326 21612 38-39 0.8879828008461566 21612 40-41 0.48692230850806695 21612 42-43 1.1808344029259281 21612 44-45 1.613152200401064 21612 46-47 1.7456606619387287 21612 48-49 1.0413680282949898 21612 50-51 0.7335387456107298 21612 52-53 0.8724341812850831 21612 54-55 1.5918958609216425 21612 56-57 1.695269564698858 21612 58-59 1.4209579361799776 21612 60-61 0.5944145338724951 21612 62-63 0.9816696416416413 21612 64-65 1.213032378719646 21612 66-67 0.47962548763010204 21612 68-69 0.84847810952839 21612 70-71 -0.5825939467140309 21612 72-73 1.0317998409807743 21612 74-75 0.8215174034938464 21612 76 2.089822542197833 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 2.0 11 12.0 12 30.0 13 65.0 14 153.0 15 245.0 16 362.0 17 575.0 18 828.0 19 1127.0 20 1600.0 21 2108.0 22 2695.0 23 3363.0 24 3883.0 25 4139.0 26 4409.0 27 4944.0 28 5234.0 29 5573.0 30 6150.0 31 6910.0 32 7877.0 33 9599.0 34 11906.0 35 12725.0 36 8043.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 57.37349005805446 12.85136337117553 6.323823369071416 23.451323201698596 2 40.90974301098922 18.28093767036162 19.863806344864525 20.945512973784634 3 37.65888462752374 17.458419809290625 20.47208699561005 24.410608567575583 4 35.4553018927475 14.722113297053282 19.271784768116913 30.55080004208231 5 36.200350048299015 23.501056839809863 22.49681991640923 17.80177319548189 6 49.38072056390294 23.276299052191625 11.91503199211913 15.427948391786298 7 46.46078215710091 21.298430521151143 11.688361372265845 20.5524259494821 8 45.09119427680595 24.021347207743148 12.744244766012796 18.143213749438107 9 40.63812083361229 15.039643448071388 11.626194324626757 32.69604139368957 10-11 44.50491119676349 18.30867373776983 16.36236693860765 20.824048126859037 12-13 43.23287776045602 17.24848647149402 16.353280985491168 23.165354782558794 14-15 43.17884024981589 16.43170710712817 14.262555352582801 26.12689729047314 16-17 43.65513547634305 18.509042914391195 14.445230831029965 23.390590778235794 18-19 42.08852587583806 19.613225322073127 15.372476256969883 22.92577254511893 20-21 43.341430989795036 18.280459462302858 15.269661524336009 23.108448023566094 22-23 43.47532924624846 18.121694386793806 14.779976472163508 23.622999894794226 24-25 43.36581960079191 18.659200244842523 14.541350650841167 23.433629503524394 26-27 43.64147613969481 18.372363267706934 15.325685155872662 22.660475436725598 28-29 43.29791405644768 18.6405501305508 15.862161309142381 22.19937450385914 30-31 43.67378559063477 18.130780339910288 15.247663953632948 22.947770115821992 32-33 43.16210296775921 18.713237755482655 15.701483401398281 22.423175875359853 34-35 42.82257524603805 18.793576709354706 15.662270340579779 22.721577704027467 36-37 43.07411268494697 17.96245110322599 16.482875369415726 22.480560842411315 38-39 42.7092399361114 18.126476467381426 17.254703176257927 21.90958042024924 40-41 42.589209713362095 18.07865566150521 17.098329141042683 22.23380548409002 42-43 41.94649808238569 17.908413592585863 17.57701540786365 22.5680729171648 44-45 40.79449486882753 18.799315206059852 17.97297168051876 22.43321824459386 46-47 41.36212783457827 18.411966678462466 17.557408877454403 22.668496609504864 48-49 40.71128666660291 18.064787627801103 18.39570760446455 22.82821810113144 50-51 41.07711583155599 17.90219688782195 18.372275409585203 22.64841187103685 52-53 39.727134481670284 19.34255956081372 18.273764549480187 22.656541408035807 54-55 40.380844897998216 18.616161519553927 17.484243044463785 23.518750537984065 56-57 40.2431209770747 18.96094952992148 17.28961236454757 23.50631712845625 58-59 40.25698901077881 19.44250504509502 17.692741758084107 22.60776418604206 60-61 40.177128264965525 19.016899872796657 17.495241829815317 23.310730032422505 62-63 40.096408127740766 19.03917058404388 17.640870159867248 23.223551128348102 64-65 40.355499870883826 19.08050154461203 17.7042187514944 22.859779833009746 66-67 40.41671050240539 19.155102001778936 16.571822068345497 23.856365427470184 68-69 40.37175894488174 18.77731763535679 16.955344931472784 23.895578488288685 70-71 40.32202530677047 18.597989613320966 17.078244402574672 24.001740677333892 72-73 41.24879252465162 17.78503591342521 16.6249031628681 24.34126839905506 74-75 41.621316602427385 17.176755262679684 17.033292845051022 24.168635289841905 76 42.23916141434815 16.31072046826133 16.72197939879683 24.728138718593687 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.5 8 2.0 9 3.0 10 5.0 11 5.5 12 5.0 13 8.5 14 13.0 15 18.5 16 29.5 17 36.0 18 42.0 19 62.5 20 91.0 21 105.0 22 121.5 23 163.5 24 232.0 25 275.0 26 304.5 27 403.5 28 549.0 29 625.0 30 720.0 31 861.0 32 1011.5 33 1116.0 34 1219.5 35 1426.0 36 1605.5 37 1682.0 38 1787.5 39 1984.5 40 2110.5 41 2149.0 42 2153.0 43 2273.0 44 2355.5 45 2306.0 46 2294.0 47 2312.0 48 2337.5 49 2223.5 50 2102.0 51 2023.0 52 1964.5 53 1929.0 54 1873.0 55 1874.5 56 1910.0 57 1982.0 58 2020.0 59 2094.5 60 2214.0 61 2365.0 62 2471.0 63 2548.0 64 2656.5 65 2682.0 66 2647.5 67 2619.0 68 2581.5 69 2537.0 70 2477.5 71 2425.0 72 2375.5 73 2294.0 74 2138.5 75 2015.0 76 1922.5 77 1652.5 78 1380.5 79 1286.0 80 1133.0 81 889.5 82 712.5 83 626.0 84 528.0 85 377.0 86 283.0 87 242.0 88 236.5 89 246.0 90 241.0 91 263.5 92 306.0 93 330.5 94 413.5 95 549.5 96 627.0 97 779.5 98 1365.5 99 9060.5 100 16322.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 4.782080587622063E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0014346241762866188 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 104557.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.59853477050795 #Duplication Level Percentage of deduplicated Percentage of total 1 85.70438178242476 57.93490631904129 2 8.438149945528375 11.408131449831192 3 3.6149351292463106 7.330929540824623 4 1.3044893108278273 3.527262641430033 5 0.5051005249083886 1.7072027697810763 6 0.19807863721897595 0.8033895387205066 7 0.06791267561793461 0.32135581548820263 8 0.03678603262638124 0.1989345524450778 9 0.019807863721897592 0.12050843080807598 >10 0.09903931860948798 1.0979657029180256 >50 0.005659389634827884 0.2658836806717867 >100 0.004244542226120913 0.5623726771043546 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.001414847408706971 14.721156880935757 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 15392 14.721156880935757 No Hit CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 257 0.24579894220377402 No Hit CTTATACACATCTGACGCTGCCGACGATCCGTGCCGTGTAGATCTCGGTG 222 0.2123243780904196 No Hit GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 109 0.10424935681016097 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.001912832235048825 0.0 4 0.0 0.0 0.0 0.005738496705146475 0.0 5 0.0 0.0 0.0 0.017215490115439425 0.0 6 0.0 0.0 0.0 0.017215490115439425 0.0 7 0.0 0.0 0.0 0.018171906232963837 0.0 8 0.0 0.0 0.0 0.01912832235048825 0.0 9 0.0 0.0 0.0 0.0229539868205859 0.0 10 0.0 0.0 0.0 0.023910402938110312 0.0 11 0.0 0.0 0.0 0.024866819055634724 0.0 12 0.0 0.0 0.0 0.03251814799583003 0.0 13 0.0 0.0 0.0 0.03443098023087885 0.0 14 0.0 0.0 0.0 0.035387396348403266 0.0 15 0.0 0.0 0.0 0.036343812465927675 0.0 16 0.0 0.0 0.0 0.0382566447009765 0.0 17 0.0 0.0 0.0 0.04208230917107415 0.0 18 0.0 0.0 0.0 0.04877722199374504 0.0 19 0.0 0.0 0.0 0.050690054228793864 0.0 20 0.0 0.0 0.0 0.059297799286513575 0.0 21 0.0 0.0 0.0 0.0612106315215624 0.0 22 0.0 0.0 0.0 0.08033895387205066 0.0 23 0.0 0.0 0.0 0.08512103445967271 0.0 24 0.0 0.0 0.0 0.09851086010501449 0.0 25 0.0 0.0 0.0 0.10329294069263656 0.0 26 0.0 0.0 0.0 0.11094426963283185 0.0 27 0.0 0.0 0.0 0.16259073997915013 0.0 28 0.0 0.0 0.0 0.2151936264429928 0.0 29 0.0 0.0 0.0 0.27831709019960404 0.0 30 0.0 0.0 0.0 0.31753015101810494 0.0 31 0.0 0.0 0.0 0.3730022858345209 0.0 32 0.0 0.0 0.0 0.4829901393498283 0.0 33 0.0 0.0 0.0 0.5920215767476114 0.0 34 0.0 0.0 0.0 0.8072152031906041 0.0 35 0.0 0.0 0.0 1.050144897041805 0.0 36 0.0 0.0 0.0 1.4355805924041432 0.0 37 0.0 0.0 0.0 1.86405501305508 0.0 38 0.0 0.0 0.0 2.315483420526603 0.0 39 0.0 0.0 0.0 2.7305680155321976 0.0 40 0.0 0.0 0.0 3.2499019673479537 0.0 41 0.0 0.0 0.0 3.7568025096358926 0.0 42 0.0 0.0 0.0 4.260833803571257 0.0 43 0.0 0.0 0.0 4.73808544621594 0.0 44 0.0 0.0 0.0 5.272722055912086 0.0 45 0.0 0.0 0.0 5.873351377717417 0.0 46 0.0 0.0 0.0 6.386946832828027 0.0 47 0.0 0.0 0.0 6.9512323421674305 0.0 48 0.0 0.0 0.0 7.411268494696673 0.0 49 0.0 0.0 0.0 7.903822795221745 0.0 50 0.0 0.0 0.0 8.376292357278805 0.0 51 0.0 0.0 0.0 8.742599730290655 0.0 52 0.0 0.0 0.0 9.147163748003482 0.0 53 0.0 0.0 0.0 9.584245913712138 0.0 54 0.0 0.0 0.0 10.018458831068221 0.0 55 0.0 0.0 0.0 10.47562573524489 0.0 56 0.0 0.0 0.0 10.909838652600973 0.0 57 0.0 0.0 0.0 11.335443824899338 0.0 58 0.0 0.0 0.0 11.734269345907018 0.0 59 0.0 0.0 0.0 12.13596411526727 0.0 60 0.0 0.0 0.0 12.526181891217231 0.0 61 0.0 0.0 0.0 12.927876660577484 0.0 62 0.0 0.0 0.0 13.362089577933567 0.0 63 0.0 0.0 0.0 13.81160515317004 0.0 64 0.0 0.0 0.0 14.240079573820978 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGTTAC 15 0.0022192365 70.0 12 GATACGT 15 0.0022192365 70.0 26 ATACGTG 15 0.0022192365 70.0 27 CGATACG 15 0.0022192365 70.0 25 GGATATT 20 0.006934462 52.5 1 TGTGATA 20 0.006934462 52.5 43 AGACACG 20 0.006934462 52.5 7 TTCGCTC 20 0.006934462 52.5 54 CCGTTCG 20 0.006934462 52.5 1 AGTGTTA 20 0.006934462 52.5 11 AGTAACT 20 0.006934462 52.5 51 GCCGTAT 70 7.2759576E-12 50.0 54 CCGTATC 80 0.0 48.125 55 CGCCGTA 80 0.0 48.125 53 CGTATCA 85 1.8189894E-12 45.294117 56 TATCATT 70 3.783498E-10 45.0 58 ATCTCGG 105 0.0 43.333336 42 CGTGTAG 105 0.0 43.333336 35 TCTCGGT 100 0.0 42.0 43 GTATCAT 95 3.6379788E-12 40.526318 57 >>END_MODULE