FastQCFastQC Report
Wed 25 May 2016
SRR1781223_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781223_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104557
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT38993.7290664422276847No Hit
TCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG15181.4518396664020583No Hit
CTTAAACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT7970.7622636456669568No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5830.5575905965167325No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC3900.3730022858345209No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCAGGATCTCGTATGCCGT2030.19415247185745577No Hit
CTAATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT2000.1912832235048825No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCC1850.17693698174201633No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGACGT1700.16259073997915013No Hit
CTTATACACATCTCCGAGCCCACGAGACATTGCCGGATCTAGTATGCCGT1500.14346241762866188No Hit
CTAAAACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCGT1230.11763918245550276No Hit
TCATATACACATCTCCGAGCCCACGAGACATTGCCGGATCTCGTATGCCG1180.11285710186788069No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTAT207.852557E-570.0761546
ACGTATT150.002209678570.0761547
TTCTCCG150.002209678570.0761546
CGTAAGA150.002213881670.042641
GTGTCTC150.002222305469.9756233
CGAATCT207.908646E-569.9756134
TCGTAAG1250.064.4083640
ACGGATC600.064.1443133
CTCGTAA1300.061.93111439
CTTAAGC308.29712E-658.36883551
CTAAGCC308.29712E-658.36883542
CGTAAGC1200.058.36883541
GACGGAT601.8189894E-1258.3130132
TCGAATG553.45608E-1157.28016740
AAAACAC1350.057.0171623
GCCGAAA252.3681913E-456.06091746
TAAGACG252.3681913E-456.06091743
AAATAAG252.3738058E-456.03407750
CAAGTAA252.3794307E-456.0072739
TAAGCCG1450.055.57763743
Produced by FastQC (version 0.11.5)