FastQCFastQC Report
Wed 25 May 2016
SRR1781222_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781222_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences242225
Sequences flagged as poor quality0
Sequence length76
%GC70

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5781323.867478583961194No Hit
CTTATACACATCTGACGCTGCCGACGATCCGTGCCGTGTAGATCTCGGTG22740.938796573433791No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6590.2720611002167406No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5190.21426359789451957No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2930.12096191557436269No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2490.1027969862730932No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATA207.91601E-570.055
CGTATAA252.3872583E-455.99999656
TAGAGAC200.00694544852.568
TATATCC200.00694544852.55
CGCTATC200.00694544852.512
GTACTCT200.00694544852.531
GCTCGCA200.00694544852.550
GCCGTAT5500.051.54545654
CGTATCA5450.050.73394456
TATCATT5150.050.29126458
TCATTAA5250.050.060
CCGTATC5400.049.90740655
ATCATTA5400.049.2592659
CATTAAA5550.047.9279361
GTATCAT5650.047.69911657
CGCCGTA5900.047.4576353
ATTAAAA5750.046.86956862
TGATCCG305.870167E-446.6666645
GCCTTAC305.870167E-446.6666641
TAGATCT5650.046.4601839
Produced by FastQC (version 0.11.5)