FastQCFastQC Report
Wed 25 May 2016
SRR1781222_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781222_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences242225
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT2477710.228919393126226No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG55782.3028176282382082No Hit
CTTAAACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT55472.2900196098668593No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42421.7512643203632987No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC20780.8578800701826814No Hit
CTAATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT13810.5701310764784808No Hit
CTAAAACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGT9550.39426153369800804No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC7840.32366601300443804No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGACGT5830.24068531324182063No Hit
TCATATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCG5640.23284136649809065No Hit
CTCTAATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC3050.12591598720198163No Hit
CTTAAACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGACGT3000.12385179069047372No Hit
CTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCCGTC2980.12302611208587057Illumina PCR Primer Index 10 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCAGT2910.12013623696975952No Hit
CTTATACACATCTCCGCGCCCACGAGACAGTAGATTATCTCGTATGCCGT2880.11889771906285478No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTAGAATATCTCGTATGCCGT2810.11600784394674372No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAGTAGATTATCTCGTATGCC2490.1027969862730932No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATTA150.002212650170.08063548
TATACCG252.8183604E-670.0661543
TCCTAAG150.002221737570.0082640
CGTAAGA550.063.69649541
CTTTATA1450.062.7401162
AACACAT17350.062.517585
AGGGGGG21600.061.23193770
CTTAAAC12050.060.687491
ATATCGT750.060.64877737
AAACACA18800.060.4874764
TTAAACA12800.060.1385082
GTTAATC352.972156E-759.98230433
TTTATAC1550.058.6923643
TCTTTAT1550.058.6923641
CTAAAAC4300.058.587371
TAAACAC14000.058.4827463
GACGTAT308.303337E-658.40052846
AACCGAG308.3748455E-658.3161312
ATCGTAT601.8189894E-1258.3161339
AAAACAC6050.056.099153
Produced by FastQC (version 0.11.5)