FastQCFastQC Report
Wed 25 May 2016
SRR1781221_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781221_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences223059
Sequences flagged as poor quality0
Sequence length76
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3022013.547985062248106No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4860.21787957446236195No Hit
CTTATACACATCTGACGCTGCCGACGATCCGTGCCGTGTAGATCTCGGTG3860.17304838630138214No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTACC150.002222547870.049
CGTATCA950.058.94737256
GCCGTAT1050.050.00000454
TCTCGGT1300.048.4615443
CCGTATC1100.047.72727255
ATCATTA1150.045.65217659
GTATCAT1200.043.7557
TAGATCT1350.041.4814839
TCATTAA1400.040.00000460
AGATCTC1600.039.37540
TATCATT1250.039.258
CGGTGGT1900.038.6842146
CATTAAA1400.037.50000461
ATCTCGG1900.035.00000442
ATGCTTA400.002415269635.06
AATGCTT400.002415269635.05
GGTATAG501.7060761E-435.01
CGCCGTA1500.035.053
GTGTAGA2000.033.2536
TCGGTGG2400.032.08333245
Produced by FastQC (version 0.11.5)