##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1781221_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 223059 Sequences flagged as poor quality 0 Sequence length 76 %GC 64 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.26140169192904 32.0 27.0 32.0 13.0 32.0 2 27.377994162979302 32.0 27.0 32.0 13.0 32.0 3 26.925149848246427 32.0 27.0 32.0 13.0 32.0 4 25.42723674005532 32.0 22.0 32.0 8.0 32.0 5 26.439484620660902 32.0 22.0 32.0 13.0 32.0 6 30.372757880202098 37.0 27.0 37.0 13.0 37.0 7 30.04098467221677 37.0 27.0 37.0 13.0 37.0 8 30.120528649370794 37.0 27.0 37.0 8.0 37.0 9 30.115229602930167 37.0 27.0 37.0 13.0 37.0 10-11 29.743312307506084 37.0 24.5 37.0 10.5 37.0 12-13 29.770737786863567 37.0 24.5 37.0 10.5 37.0 14-15 30.527223739010758 37.0 27.0 37.0 10.5 37.0 16-17 30.815707951707843 37.0 29.5 37.0 13.0 37.0 18-19 30.062239138523886 37.0 24.5 37.0 13.0 37.0 20-21 30.441495299449922 37.0 27.0 37.0 13.0 37.0 22-23 30.39735451158662 37.0 27.0 37.0 13.0 37.0 24-25 29.635038263419098 37.0 22.0 37.0 8.0 37.0 26-27 30.15073814551307 37.0 24.5 37.0 10.5 37.0 28-29 29.653965991060662 37.0 24.5 37.0 8.0 37.0 30-31 29.88970182776754 37.0 27.0 37.0 8.0 37.0 32-33 29.90849282028522 37.0 27.0 37.0 8.0 37.0 34-35 29.870771858566567 37.0 24.5 37.0 10.5 37.0 36-37 30.54861942356058 37.0 27.0 37.0 13.0 37.0 38-39 30.209146459008604 37.0 24.5 37.0 13.0 37.0 40-41 29.372311809879896 37.0 24.5 37.0 8.0 37.0 42-43 29.463621732366775 37.0 22.0 37.0 8.0 37.0 44-45 29.204477290761638 37.0 22.0 37.0 8.0 37.0 46-47 29.604938603687813 37.0 24.5 37.0 10.5 37.0 48-49 30.09020035057989 37.0 24.5 37.0 13.0 37.0 50-51 30.275420404466978 37.0 27.0 37.0 13.0 37.0 52-53 29.193872473202156 37.0 22.0 37.0 10.5 37.0 54-55 29.424347369978346 37.0 24.5 37.0 8.0 37.0 56-57 29.17271663550899 37.0 22.0 37.0 8.0 37.0 58-59 28.62235552028835 37.0 17.5 37.0 8.0 37.0 60-61 28.427451929758494 37.0 17.5 37.0 8.0 37.0 62-63 28.06028449872007 37.0 13.0 37.0 8.0 37.0 64-65 27.906957352090703 37.0 13.0 37.0 8.0 37.0 66-67 28.045568661206225 37.0 13.0 37.0 8.0 37.0 68-69 28.420252040939843 37.0 17.5 37.0 8.0 37.0 70-71 28.498182095320075 37.0 22.0 37.0 8.0 37.0 72-73 28.81353139752263 37.0 22.0 37.0 8.0 37.0 74-75 29.180216893288325 37.0 22.0 37.0 8.0 37.0 76 29.00257331020044 37.0 22.0 37.0 8.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 11101 1 -0.6146155353412581 11101 2 -0.7601329128007634 11101 3 -0.7326258764048177 11101 4 -2.5152600238245313 11101 5 -0.9326979452966775 11101 6 -1.6775065957436048 11101 7 0.07427891451513347 11101 8 -2.850211416826948 11101 9 -1.3043380061304433 11101 10-11 -1.8129671561602478 11101 12-13 -0.23131382501042808 11101 14-15 -0.7675185103383484 11101 16-17 -1.1486218355857254 11101 18-19 -1.3644638539451286 11101 20-21 -1.0502531494164344 11101 22-23 0.07260860610555753 11101 24-25 -1.0685574611676003 11101 26-27 -1.5380009210143086 11101 28-29 0.08950243183565121 11101 30-31 -3.1415895425108644 11101 32-33 -1.1561069900408434 11101 34-35 -0.5815648201326411 11101 36-37 -0.9540490256103595 11101 38-39 -0.8963007676999517 11101 40-41 -0.31338259998547 11101 42-43 0.6676536402418058 11101 44-45 -1.53685740778646 11101 46-47 -2.748750826603839 11101 48-49 -1.4573806741131392 11101 50-51 -2.0718015188689556 11101 52-53 -1.5906497004294486 11101 54-55 -2.3487455286328967 11101 56-57 -2.5522123629165634 11101 58-59 -1.7211218996908109 11101 60-61 -1.11751210175035 11101 62-63 -1.4569514113536641 11101 64-65 -1.9071303443805405 11101 66-67 -0.9715235114898455 11101 68-69 -1.0531038452010115 11101 70-71 -0.5647757797078192 11101 72-73 -1.5884985308933643 11101 74-75 -2.554613416886415 11101 76 -1.6946113285275004 11102 1 -0.3195684712444411 11102 2 -0.09888235251389688 11102 3 -0.6704340341816 11102 4 0.16391971620236134 11102 5 -1.5866647763141586 11102 6 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2.114780572095203 21610 76 1.8908356795999204 21611 1 -0.14722483023072286 21611 2 -0.4233999065710954 21611 3 0.25673406626015804 21611 4 0.36323382919934133 21611 5 0.318473563606787 21611 6 -0.3304091813326693 21611 7 0.033041801113071756 21611 8 -0.8126680020852284 21611 9 0.24982705258282678 21611 10-11 0.07161854316165872 21611 12-13 1.1176304387306857 21611 14-15 0.6632384222378711 21611 16-17 0.8988173837758673 21611 18-19 0.12643972398089076 21611 20-21 0.7401707989820423 21611 22-23 0.570127763721743 21611 24-25 0.48053565311653657 21611 26-27 -0.6382820831511395 21611 28-29 0.4465232157818484 21611 30-31 -0.3347644694638383 21611 32-33 0.990290275519552 21611 34-35 0.6389545028730339 21611 36-37 0.5356692609544993 21611 38-39 0.15802968050557098 21611 40-41 1.089616462807406 21611 42-43 0.1793687292764794 21611 44-45 0.6110283632042233 21611 46-47 1.39285014368118 21611 48-49 1.093421464924294 21611 50-51 1.0200999089936609 21611 52-53 1.0040909137168654 21611 54-55 0.16787611533865743 21611 56-57 0.4453343596149999 21611 58-59 1.0299669144665309 21611 60-61 0.6933374489415343 21611 62-63 1.2057918490245285 21611 64-65 0.507115203885629 21611 66-67 -0.12776145183936904 21611 68-69 0.7840701996746695 21611 70-71 -0.2651451495738506 21611 72-73 1.199003536475292 21611 74-75 1.2192991472020118 21611 76 1.7562404273576107 21612 1 0.2703306399734302 21612 2 0.30269103206452996 21612 3 1.2556409117708398 21612 4 1.228716737581177 21612 5 0.08592710454099262 21612 6 0.6767110952058744 21612 7 -0.5691681436383078 21612 8 0.9495265794138703 21612 9 0.49927308915918545 21612 10-11 0.8748211903682055 21612 12-13 0.9287362974478519 21612 14-15 1.9776828338548498 21612 16-17 1.1251151354229876 21612 18-19 0.49693046171244504 21612 20-21 1.0093527057980118 21612 22-23 0.5923158453833359 21612 24-25 0.42500635953069477 21612 26-27 0.7743246471351455 21612 28-29 0.24334420434804116 21612 30-31 0.5931316513149625 21612 32-33 1.3852440380391258 21612 34-35 0.9773005896754086 21612 36-37 0.850217079077229 21612 38-39 0.858298537420513 21612 40-41 0.5218113397536648 21612 42-43 1.646721818176534 21612 44-45 0.7650449673345747 21612 46-47 1.1227818953873872 21612 48-49 0.8906856242294054 21612 50-51 0.9048459721602633 21612 52-53 0.45300809987811874 21612 54-55 0.6910391726258638 21612 56-57 1.287595121277775 21612 58-59 1.0802963380491235 21612 60-61 1.1363752366332065 21612 62-63 0.019866951692414148 21612 64-65 0.8079690461759341 21612 66-67 0.7831612296390489 21612 68-69 1.2998323056220187 21612 70-71 0.9442234798467766 21612 72-73 1.9658957278068705 21612 74-75 0.8091446283377124 21612 76 0.3014586150847336 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 3.0 11 19.0 12 74.0 13 145.0 14 348.0 15 554.0 16 870.0 17 1354.0 18 2117.0 19 2959.0 20 4414.0 21 6382.0 22 8162.0 23 9933.0 24 10938.0 25 11690.0 26 12299.0 27 12855.0 28 13144.0 29 13581.0 30 14380.0 31 15018.0 32 16022.0 33 17270.0 34 18083.0 35 16905.0 36 13540.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 55.835899918855546 13.588333131592986 6.49065942194666 24.085107527604805 2 38.573202605588655 18.464621467862763 21.67049973325443 21.29167619329415 3 36.944933851581865 18.11180001703585 19.908185726646312 25.035080404735965 4 34.17974616581263 15.6021501037842 19.571503503557356 30.64660022684581 5 35.26869572624283 25.49280683585957 22.916806764129667 16.32169067376793 6 49.89666411128894 24.16042392371525 11.138757010477049 14.804154954518758 7 46.71072097840024 21.44957813662814 11.435142429323315 20.404558455648306 8 44.351494447657345 24.841857983762143 12.518212670190398 18.28843489839011 9 39.995247894054934 14.976755028938532 11.266077584854232 33.761919492152295 10-11 44.26731104172224 19.10956094477458 16.022030095244073 20.60109791825911 12-13 42.820509371959886 17.757409474623305 16.073998359178514 23.348082794238294 14-15 42.194441829291804 16.978691736267088 14.14491233261155 26.681954101829557 16-17 43.09465208756428 18.933107384145 14.104340107325864 23.867900420964858 18-19 42.01498709979669 19.652243693918862 15.003014904161912 23.329754302122538 20-21 42.779360571329946 18.67581822705703 15.028792895137375 23.516028306475654 22-23 43.06199074229739 18.755253690191992 14.468018336079261 23.714737231431357 24-25 43.348717937222744 18.978205269021355 13.990500249934435 23.68257654382146 26-27 42.63983968367114 18.98668065399737 15.404668719935085 22.968810942396406 28-29 42.81333638185413 19.25880596613452 15.806804477739073 22.12105317427228 30-31 43.01530088451934 18.802424470655748 15.210325519257237 22.971949125567676 32-33 42.470153636481825 19.289067018143182 15.594080489915225 22.646698855459764 34-35 42.54236034367974 18.96425345147978 15.557493280850368 22.93589292399011 36-37 42.96368192883433 18.16396705774757 16.163805664023098 22.708545349395 38-39 41.96105945538828 18.548090631136297 17.352437482627835 22.13841243084758 40-41 41.915107281514224 18.5539187117252 17.048928978113313 22.482045028647256 42-43 41.376272645353914 18.19877252206815 17.679627363164006 22.745327469413922 44-45 40.34053846860801 18.5617225974352 18.312908945411184 22.784829988545603 46-47 40.53573630117795 18.258072470103002 17.94492451497932 23.26126671373973 48-49 39.906706297437 18.435481195558125 18.77149095082467 22.886321556180203 50-51 39.802922096844334 18.57513034667958 18.583872428370967 23.03807512810512 52-53 39.06208671248414 19.793417884954202 18.368010257375854 22.776485145185806 54-55 39.23222107155506 19.13103707987573 17.66841956612376 23.96832228244545 56-57 39.11924647738939 19.643233404614925 17.58099874920985 23.65652136878584 58-59 39.47341286386113 19.895184682079627 18.03805271251104 22.5933497415482 60-61 39.281983690413746 19.743655266095516 17.357963588108976 23.61639745538176 62-63 39.42225788808292 19.994127088021948 17.3909924772929 23.19262254660223 64-65 39.699586208133276 20.248230288847346 17.30573525390143 22.746448249117947 66-67 39.79987312745311 20.018739478656943 16.334280020712054 23.847107373177888 68-69 39.85559874293349 19.847215310747384 16.47053021846238 23.826655727856753 70-71 40.04747622826248 19.391282127150216 16.505274389287138 24.055967255300168 72-73 40.76105595617293 18.387777197461652 16.45285877919918 24.398308067166237 74-75 41.234474364348365 17.74512067462123 16.672532093598765 24.34787286743164 76 42.21125352485217 16.282687540067876 16.44990787190833 25.056151063171626 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 1.5 4 3.0 5 1.5 6 1.5 7 5.0 8 7.0 9 8.5 10 12.5 11 17.0 12 19.0 13 19.5 14 26.0 15 40.0 16 59.5 17 71.0 18 86.0 19 124.5 20 178.5 21 209.0 22 254.5 23 354.5 24 476.0 25 543.0 26 652.0 27 838.5 28 1057.0 29 1198.0 30 1369.5 31 1685.0 32 2008.0 33 2187.0 34 2414.5 35 2825.0 36 3224.5 37 3441.0 38 3646.0 39 4004.5 40 4351.5 41 4737.5 42 4930.0 43 4950.0 44 5094.5 45 5269.0 46 5319.0 47 5267.0 48 5180.0 49 4905.5 50 4666.0 51 4586.5 52 4503.0 53 4395.0 54 4291.0 55 4344.5 56 4453.0 57 4520.0 58 4532.0 59 4780.5 60 5132.5 61 5318.5 62 5401.0 63 5535.5 64 5708.5 65 5650.5 66 5520.5 67 5487.0 68 5474.5 69 5360.0 70 5156.5 71 5055.0 72 5002.0 73 4780.5 74 4410.0 75 4208.0 76 4054.5 77 3679.0 78 3183.5 79 2910.0 80 2658.5 81 2210.5 82 1769.0 83 1524.0 84 1368.5 85 1087.5 86 841.5 87 721.0 88 694.0 89 626.0 90 570.0 91 610.5 92 666.0 93 656.0 94 747.0 95 961.5 96 1075.0 97 1390.0 98 2472.5 99 17660.5 100 32081.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 4.483118816097983E-4 8 0.0 9 0.0 10-11 2.2415594080489915E-4 12-13 0.0 14-15 0.0 16-17 0.0 18-19 2.2415594080489915E-4 20-21 2.2415594080489915E-4 22-23 6.724678224146975E-4 24-25 2.2415594080489915E-4 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0011207797040244957 36-37 8.966237632195966E-4 38-39 4.483118816097983E-4 40-41 4.483118816097983E-4 42-43 0.0 44-45 2.2415594080489915E-4 46-47 6.724678224146975E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 4.483118816097983E-4 64-65 0.0 66-67 2.2415594080489915E-4 68-69 0.0 70-71 0.0 72-73 2.2415594080489915E-4 74-75 2.2415594080489915E-4 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 223059.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.2580084409605 #Duplication Level Percentage of deduplicated Percentage of total 1 89.50983127003052 64.67802143458815 2 6.395173916053164 9.242050616151598 3 2.462694254429945 5.338481466723116 4 0.9337315013192781 2.698783148156765 5 0.36306940743835864 1.3117336153667725 6 0.14401297563419185 0.6243654485389964 7 0.05432771836566451 0.2747928912571018 8 0.03243619111719321 0.1875019657231004 9 0.017457866416026643 0.11353235929653727 >10 0.07978523984511146 1.0687867097312744 >50 0.002493219783520208 0.1243070163523133 >100 0.004363134621160364 0.7269258130167886 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 6.23304945880052E-4 13.610717515097493 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 30220 13.547985062248106 No Hit CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 486 0.21787957446236195 No Hit CTTATACACATCTGACGCTGCCGACGATCCGTGCCGTGTAGATCTCGGTG 386 0.17304838630138214 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 4.483118816097983E-4 0.0 0.0 0.0 0.0 7 4.483118816097983E-4 0.0 0.0 8.966237632195966E-4 0.0 8 4.483118816097983E-4 0.0 0.0 8.966237632195966E-4 0.0 9 4.483118816097983E-4 0.0 0.0 0.004034806934488185 0.0 10 4.483118816097983E-4 0.0 0.0 0.005828054460927378 0.0 11 4.483118816097983E-4 0.0 0.0 0.006276366342537176 0.0 12 4.483118816097983E-4 0.0 0.0 0.006276366342537176 0.0 13 4.483118816097983E-4 0.0 0.0 0.007172990105756773 0.0 14 4.483118816097983E-4 0.0 0.0 0.008966237632195966 0.0 15 4.483118816097983E-4 0.0 0.0 0.01031117327702536 0.0 16 4.483118816097983E-4 0.0 0.0 0.01031117327702536 0.0 17 4.483118816097983E-4 0.0 0.0 0.01031117327702536 0.0 18 4.483118816097983E-4 0.0 0.0 0.012552732685074352 0.0 19 4.483118816097983E-4 0.0 0.0 0.02062234655405072 0.0 20 4.483118816097983E-4 0.0 0.0 0.02331221784370951 0.0 21 4.483118816097983E-4 0.0 0.0 0.0264504010149781 0.0 22 4.483118816097983E-4 0.0 0.0 0.03227845547590548 0.0 23 4.483118816097983E-4 0.0 0.0 0.03272676735751528 0.0 24 4.483118816097983E-4 0.0 0.0 0.03765819805522306 0.0 25 4.483118816097983E-4 0.0 0.0 0.04169300498971124 0.0 26 4.483118816097983E-4 0.0 0.0 0.04707274756902882 0.0 27 4.483118816097983E-4 0.0 0.0 0.08473094562425187 0.0 28 4.483118816097983E-4 0.0 0.0 0.13628681200937867 0.0 29 4.483118816097983E-4 0.0 0.0 0.1873943665128957 0.0 30 4.483118816097983E-4 0.0 0.0 0.24612322300377926 0.0 31 4.483118816097983E-4 0.0 0.0 0.3026105200866139 0.0 32 4.483118816097983E-4 0.0 0.0 0.3765819805522306 0.0 33 4.483118816097983E-4 0.0 0.0 0.4702791638086784 0.0 34 4.483118816097983E-4 0.0 0.0 0.6397410550571822 0.0 35 4.483118816097983E-4 0.0 0.0 0.8558273819931049 0.0 36 4.483118816097983E-4 0.0 0.0 1.1790602486337696 0.0 37 4.483118816097983E-4 0.0 0.0 1.6197508282562012 0.0 38 4.483118816097983E-4 0.0 0.0 2.1039276603947834 0.0 39 4.483118816097983E-4 0.0 0.0 2.549101358833313 0.001344935644829395 40 4.483118816097983E-4 0.0 0.0 3.1408730425582467 0.001344935644829395 41 4.483118816097983E-4 0.0 0.0 3.6730192460290776 0.001344935644829395 42 4.483118816097983E-4 0.0 0.0 4.268377424806889 0.001344935644829395 43 4.483118816097983E-4 0.0 0.0 4.825629093647869 0.001344935644829395 44 4.483118816097983E-4 0.0 0.0 5.459990406125733 0.0017932475264391932 45 4.483118816097983E-4 0.0 0.0 6.029794807651787 0.0017932475264391932 46 4.483118816097983E-4 0.0 0.0 6.612600253744525 0.0017932475264391932 47 4.483118816097983E-4 0.0 0.0 7.149229576031454 0.0017932475264391932 48 4.483118816097983E-4 0.0 0.0 7.676892660686186 0.0017932475264391932 49 4.483118816097983E-4 0.0 0.0 8.198727690879991 0.0017932475264391932 50 4.483118816097983E-4 0.0 0.0 8.656454122003595 0.0017932475264391932 51 4.483118816097983E-4 0.0 0.0 9.126733285812273 0.0017932475264391932 52 4.483118816097983E-4 0.0 0.0 9.592081018923244 0.0017932475264391932 53 4.483118816097983E-4 0.0 0.0 10.021563801505431 0.0017932475264391932 54 4.483118816097983E-4 0.0 0.0 10.441632034573813 0.0017932475264391932 55 4.483118816097983E-4 0.0 0.0 10.889495604302 0.0017932475264391932 56 4.483118816097983E-4 0.0 0.0 11.289389802697942 0.0017932475264391932 57 4.483118816097983E-4 0.0 0.0 11.689284001093881 0.0017932475264391932 58 4.483118816097983E-4 0.0 0.0 12.073487283633478 0.0017932475264391932 59 4.483118816097983E-4 0.0 0.0 12.446034457251221 0.0017932475264391932 60 4.483118816097983E-4 0.0 0.0 12.759852774378079 0.0017932475264391932 61 4.483118816097983E-4 0.0 0.0 13.09563837370382 0.0017932475264391932 62 4.483118816097983E-4 0.0 0.0 13.441286834424973 0.0017932475264391932 63 4.483118816097983E-4 0.0 0.0 13.798591404067983 0.0017932475264391932 64 4.483118816097983E-4 0.0 0.0 14.149171295486845 0.0017932475264391932 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGGTACC 15 0.0022225478 70.0 49 CGTATCA 95 0.0 58.947372 56 GCCGTAT 105 0.0 50.000004 54 TCTCGGT 130 0.0 48.46154 43 CCGTATC 110 0.0 47.727272 55 ATCATTA 115 0.0 45.652176 59 GTATCAT 120 0.0 43.75 57 TAGATCT 135 0.0 41.48148 39 TCATTAA 140 0.0 40.000004 60 AGATCTC 160 0.0 39.375 40 TATCATT 125 0.0 39.2 58 CGGTGGT 190 0.0 38.68421 46 CATTAAA 140 0.0 37.500004 61 ATCTCGG 190 0.0 35.000004 42 ATGCTTA 40 0.0024152696 35.0 6 AATGCTT 40 0.0024152696 35.0 5 GGTATAG 50 1.7060761E-4 35.0 1 CGCCGTA 150 0.0 35.0 53 GTGTAGA 200 0.0 33.25 36 TCGGTGG 240 0.0 32.083332 45 >>END_MODULE