FastQCFastQC Report
Wed 25 May 2016
SRR1781221_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781221_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences223059
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT116675.230454722741517No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG32221.44446088254677No Hit
CTTAAACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT26391.1830950555682578No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10610.47565890638799596No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC9140.4097570597913556No Hit
CTAATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT7730.3465450844843741No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCC4870.21832788634397177No Hit
CTAAAACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCGT4740.21249983188304442No Hit
TCATATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGCCG3410.15287435162894122No Hit
CTTATACACATCTCCGAGCCCACGAGACGTGATAGCATCTCGTATGACGT2360.1058016040599124No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTAT150.002214199970.0664346
AAATCCG207.92142E-569.9878910
TTAATAC352.9685725E-759.9896243
AACACAT9250.057.5035675
AGGGGGG13550.056.55848370
CTTAAAC5300.056.1223641
TCGTATC252.3888872E-455.99031440
AACTATT252.3888872E-455.99031419
AAACACA10300.055.3787734
CTAAAAC3250.053.836841
AAAACAC4550.053.067753
TTAAACA6200.053.055342
ATATGTC200.006918721452.5498248
TAGAGTA200.00694948252.4909232
TCCGATG200.00694948252.490923
GTAATGC200.00694948252.4909224
TCGTAAT200.00694948252.4909240
CACGTAT407.465842E-752.4909239
CGTAAGC1350.051.8661441
TAAACAC6600.051.43053