FastQCFastQC Report
Wed 25 May 2016
SRR1781220_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781220_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences202275
Sequences flagged as poor quality0
Sequence length76
%GC72

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5019024.812754912866147No Hit
CTTATACACATCTGACGCTGCCGACGATCCGTGCCGTGTAGATCTCGGTG12940.6397231491780991No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6140.303547151155605No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3720.1839080459770115No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2580.12754912866147572No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC2200.055.6818255
CGTATCA2200.054.0909156
TATACCC200.00694379952.54
TAACTCT200.00694379952.570
GCCGTAT2300.051.7391354
ATCATTA2200.050.90909659
TCATTAA2200.049.31818460
GTATCAT2350.049.14893757
TATCATT2550.046.66666858
TAGATCT2450.045.71428739
CATTAAA2350.044.6808561
GTAGATC2550.043.9215738
ATTAAAA2450.042.85714362
AGATCTC2600.041.7307740
TGTAGAT2600.041.7307737
CGCCGTA3000.040.83333253
ATCTCGG2750.040.72727242
TCGGTGG2750.040.72727245
TCTCGGT2650.039.6226443
CGCGTCT459.1750284E-538.888897