FastQCFastQC Report
Wed 25 May 2016
SRR1781220_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781220_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences202275
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT190719.42825361512792No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG54342.6864417253738724No Hit
CTTAAACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT53632.651340996168582No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23471.1603015696452847No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC17640.8720800889877641No Hit
CTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT12170.6016561611667285No Hit
CTAAAACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGT9850.48696082066493634No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC7940.39253491533802987No Hit
TCATATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCG5920.29267086886664195No Hit
CTTATACACATCTCCGAGCCCACGAGACTACATGAAATCTCGTATGCCGT3510.1735261401557286No Hit
ATACCCATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT3330.1646273637374861TruSeq Adapter, Index 12 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACTACTAGAAATCTCGTATGCCGT3290.16264985786676553No Hit
CTCTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC3190.15770609318996415No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGACGT2850.14089729328883943No Hit
CTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTC2670.13199851687059697TruSeq Adapter, Index 12 (95% over 21bp)
CTTATACACATCTCCGCGCCCACGAGACTACTTGAAATCTCGTATGCCGT2310.11420096403411198No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCCGTCTT2270.11222345816339141TruSeq Adapter, Index 12 (95% over 23bp)
TCTTAATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCC2130.10530218761586947No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGAAATCTCGTATGCAGT2070.10233592880978866No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAATA207.8748344E-570.0680748
CGACTTA207.8748344E-570.0680748
CGAAAGA207.884497E-570.0507341
TCACACG150.00222458369.9814622
TGCGTAC150.00222458369.981468
CTTTATA1500.067.648742
TAAAACA4750.064.824932
CTAAAAC4400.063.619511
CTTAAAC11250.062.8277971
AGGGGGG20700.062.5438170
AACACAT16750.062.4610675
TAAACAC12700.061.715933
TCTTTAT1650.061.498861
TTAAACA11900.061.4543082
AAACACA17900.061.1849064
AAACTCG1200.058.31788336
TTAATAC308.368381E-658.3178833
TTTATAC1750.057.9846423
TATCGTA1450.057.9300138
TCGAAAG800.056.87399740
Produced by FastQC (version 0.11.5)