FastQCFastQC Report
Wed 25 May 2016
SRR1781219_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781219_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences324021
Sequences flagged as poor quality0
Sequence length76
%GC67

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6113218.8666783943016No Hit
CTTATACACATCTGACGCTGCCGACGATCCGTGCCGTGTAGATCTCGGTG11620.35861873150197054No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6610.20399912351359942No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5300.16356964517731876No Hit
GGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3340.1030797386589141No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATACG200.006947977552.4991925
GTTCGTT200.006947977552.4991921
CCGTATC2450.048.57067555
CGTATCA2550.048.03847556
GCCGTAT2550.048.03847554
GTATCAT2650.044.9049757
TCATTAA2650.042.263560
TTGTGCG503.4698096E-642.0058332
TAGATCT3100.041.7735539
TATCATT2700.041.4808458
CGCCGTA3050.040.1633253
ATCATTA2950.037.96551559
CATTAAA3050.036.7207561
AGATCTC3450.036.52117540
GTAGATC3700.035.94539338
TCTCGGT3450.035.506743
TGTGCGC806.2198524E-835.0048643
GGCATAG400.002415231435.0048641
TCGGTGG4000.034.12447445
TGTAGAT4150.032.8910637