FastQCFastQC Report
Wed 25 May 2016
SRR1781219_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781219_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences324021
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT236507.298909638572809TruSeq Adapter, Index 5 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG57471.7736504732717941TruSeq Adapter, Index 5 (95% over 22bp)
CTTAAACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT48481.4961993204144175RNA PCR Primer, Index 5 (95% over 23bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23620.7289650979411828No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC21410.6607596421219613RNA PCR Primer, Index 5 (95% over 21bp)
CTAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT13300.41046722280346026TruSeq Adapter, Index 5 (95% over 23bp)
CTAAAACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT7420.22899750324824625No Hit
TCTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC7380.22776301536011553RNA PCR Primer, Index 5 (95% over 21bp)
CTTATACACATCTCCGCGCCCACGAGACACAAAGTGATCTCGTATGCCGT5940.18332145138741007TruSeq Adapter, Index 5 (95% over 23bp)
TCATATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG5620.17344554828236441TruSeq Adapter, Index 5 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGACGT4680.14443508291129278No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCACGTATGCCGT4040.12468327670120147No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAAGAGATCTCGTATGCCGT3640.11233839781989438No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGTA150.00222616369.9785512
AGGGGGG21350.059.3260870
ACGAAAG308.358804E-658.34246440
AACACAT17450.057.3463175
AAACACA18600.056.9986534
ACGATAC252.3920856E-455.9828422
CTTAAAC11100.055.7937131
CTAAAAC5050.055.4285551
TTAAACA12800.053.850682
TAAACAC13350.053.7288483
AAAACAC6700.052.7450263
TAAGCCG2800.052.54878643
GCGTACC200.006955980352.48391311
TAAAACA6050.052.0501632
CACGTAT3600.051.53584339
ATTAAAC1450.050.6741261
AAGCCGT3000.050.21328744
GTAAGCC2900.049.52874442
CGTAAGC3050.049.39011841
TAAGACG506.137998E-849.04553243
Produced by FastQC (version 0.11.5)