##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1781219_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 324021 Sequences flagged as poor quality 0 Sequence length 76 %GC 55 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.855515537573183 32.0 27.0 32.0 22.0 32.0 2 27.72337904024739 32.0 27.0 32.0 13.0 32.0 3 28.39798963647418 32.0 27.0 32.0 13.0 32.0 4 29.327784310276186 32.0 27.0 32.0 22.0 32.0 5 27.055385299100983 32.0 27.0 32.0 13.0 32.0 6 31.760910558266286 37.0 32.0 37.0 13.0 37.0 7 31.405896531397655 37.0 27.0 37.0 13.0 37.0 8 31.834837865446993 37.0 32.0 37.0 13.0 37.0 9 32.87591544992454 37.0 32.0 37.0 22.0 37.0 10-11 32.51326920168754 37.0 32.0 37.0 17.5 37.0 12-13 32.58916860326954 37.0 32.0 37.0 22.0 37.0 14-15 32.52959993333765 37.0 32.0 37.0 22.0 37.0 16-17 31.34873819906734 37.0 29.5 37.0 13.0 37.0 18-19 31.732579061233686 37.0 29.5 37.0 13.0 37.0 20-21 32.134449619006176 37.0 32.0 37.0 22.0 37.0 22-23 32.07749652028726 37.0 32.0 37.0 17.5 37.0 24-25 32.1423704019184 37.0 32.0 37.0 13.0 37.0 26-27 32.71017464917397 37.0 32.0 37.0 13.0 37.0 28-29 32.570100394727504 37.0 32.0 37.0 13.0 37.0 30-31 32.258034201486936 37.0 32.0 37.0 13.0 37.0 32-33 32.01426450754735 37.0 32.0 37.0 13.0 37.0 34-35 31.999496946185587 37.0 32.0 37.0 13.0 37.0 36-37 31.91435585965107 37.0 32.0 37.0 13.0 37.0 38-39 31.14287808506239 37.0 27.0 37.0 13.0 37.0 40-41 30.718589227241445 37.0 27.0 37.0 13.0 37.0 42-43 31.115088219590707 37.0 27.0 37.0 13.0 37.0 44-45 31.556351285873447 37.0 29.5 37.0 13.0 37.0 46-47 30.73297101113817 37.0 27.0 37.0 13.0 37.0 48-49 30.280259612802872 37.0 27.0 37.0 13.0 37.0 50-51 30.414551834603312 37.0 27.0 37.0 13.0 37.0 52-53 30.610551476601824 37.0 27.0 37.0 13.0 37.0 54-55 30.490377166912022 37.0 27.0 37.0 13.0 37.0 56-57 29.864528842266395 37.0 24.5 37.0 13.0 37.0 58-59 30.487088799800013 37.0 27.0 37.0 13.0 37.0 60-61 30.202513108718264 37.0 27.0 37.0 13.0 37.0 62-63 30.852062057706135 37.0 27.0 37.0 13.0 37.0 64-65 31.11743528968801 37.0 27.0 37.0 13.0 37.0 66-67 31.197533493199515 37.0 27.0 37.0 13.0 37.0 68-69 30.93805185466374 37.0 27.0 37.0 13.0 37.0 70-71 29.00767542844445 34.5 20.0 37.0 13.0 37.0 72-73 28.743720314424067 37.0 17.5 37.0 10.5 37.0 74-75 30.154395857058645 37.0 27.0 37.0 13.0 37.0 76 30.32178778535959 37.0 27.0 37.0 13.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 11101 1 -1.1029826306567543 11101 2 -1.76645414443119 11101 3 -0.12403930445448808 11101 4 -1.3027165776485212 11101 5 -0.8889842048345287 11101 6 -0.7425327885880755 11101 7 -0.9074167880399244 11101 8 -1.6262097635514188 11101 9 -1.0752888108579128 11101 10-11 -1.3016257128332356 11101 12-13 -1.2401241760140067 11101 14-15 -0.8214744086427572 11101 16-17 -0.8820412454058797 11101 18-19 0.14531475604330524 11101 20-21 -0.029134795689301995 11101 22-23 -0.27953590799042516 11101 24-25 -0.30439241239078285 11101 26-27 -1.0646435238597434 11101 28-29 -0.2165912341321956 11101 30-31 -1.2766427803402465 11101 32-33 -1.1362218860781184 11101 34-35 -1.3526258399037232 11101 36-37 -1.6839757062102976 11101 38-39 -1.332642101428224 11101 40-41 -1.229896904549804 11101 42-43 -0.9892341777650238 11101 44-45 -1.3786750400784413 11101 46-47 -1.370828281323675 11101 48-49 -1.7697965937548759 11101 50-51 -1.6885802123454887 11101 52-53 -1.8118287415545709 11101 54-55 -0.5519474283934187 11101 56-57 -0.22003513665030994 11101 58-59 -0.2518430746489173 11101 60-61 -0.5170365121958866 11101 62-63 -1.2622226781129555 11101 64-65 -2.1000797490736183 11101 66-67 -1.0383829638963533 11101 68-69 -1.2436327062644246 11101 70-71 -1.223841638213898 11101 72-73 -0.4976754476550056 11101 74-75 -1.2501576456342072 11101 76 -2.3926946125498 11102 1 -0.9797568869274151 11102 2 -2.552814245539494 11102 3 -0.4168158939936646 11102 4 -0.9919057668377107 11102 5 -0.4373995266311468 11102 6 -2.3503978478919834 11102 7 -2.2021863777735433 11102 8 -0.9926301407631613 11102 9 -2.088316226763027 11102 10-11 -1.1797124326465749 11102 12-13 -1.6760079014660754 11102 14-15 -0.5207258183258219 11102 16-17 -0.015742392596237664 11102 18-19 -0.31018300790769615 11102 20-21 -1.1605755890006009 11102 22-23 -0.3507492076209928 11102 24-25 -1.034170751881355 11102 26-27 -0.35868396718075957 11102 28-29 -0.9116135946221817 11102 30-31 -0.5236775849290112 11102 32-33 -1.6381419716312955 11102 34-35 -0.9203149318733921 11102 36-37 -2.28262921583503 11102 38-39 -0.5241645591377306 11102 40-41 -1.3141133152050628 11102 42-43 -1.2861182920951784 11102 44-45 -1.3553666597556742 11102 46-47 -2.3154131539661016 11102 48-49 -1.9506735138404316 11102 50-51 -2.1621365024485435 11102 52-53 -1.8948056033084129 11102 54-55 -2.196099674164369 11102 56-57 -1.2689851658161686 11102 58-59 -2.8714230863152608 11102 60-61 -1.705934042820033 11102 62-63 -2.325852855052485 11102 64-65 -1.4256922320602037 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11104 24-25 -0.6509434068893043 11104 26-27 -1.007491598341332 11104 28-29 -1.064475279836806 11104 30-31 -1.075267410048248 11104 32-33 -0.1220449922990241 11104 34-35 -1.5984150908559016 11104 36-37 -1.0974543350719124 11104 38-39 -1.3582663079453638 11104 40-41 -0.100083108527798 11104 42-43 -1.3935507245276106 11104 44-45 -1.6898261192151338 11104 46-47 -1.9203416118357381 11104 48-49 -1.6331059462728632 11104 50-51 -1.8795450875041873 11104 52-53 -1.1979057622909224 11104 54-55 0.11284309213134236 11104 56-57 -1.6880630672470076 11104 58-59 -1.293760129240539 11104 60-61 -1.5906717216753634 11104 62-63 -0.7680627119682235 11104 64-65 -1.4777987503431937 11104 66-67 -1.2746715800622432 11104 68-69 -1.5571113351260415 11104 70-71 -1.9710697380869426 11104 72-73 -1.3673961416880118 11104 74-75 -1.9605046621386535 11104 76 -1.9755977018430713 11105 1 -1.3543815115520381 11105 2 -1.2699313626566102 11105 3 -0.9830321102098836 11105 4 -0.6971610220929669 11105 5 0.22040828117665967 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4 0.7484645247136825 21610 5 1.5445115182720244 21610 6 1.0418664636636628 21610 7 1.0912803172679553 21610 8 1.2961547070768056 21610 9 0.6194939456140744 21610 10-11 0.6585510276641315 21610 12-13 0.6123513239010236 21610 14-15 -0.2113514833609358 21610 16-17 0.6498513507534156 21610 18-19 0.6581879220774098 21610 20-21 0.5307417125529241 21610 22-23 0.9190422373673073 21610 24-25 0.1182552962139809 21610 26-27 0.1553615744238357 21610 28-29 0.2353857669441055 21610 30-31 0.5383036876821876 21610 32-33 -0.13061377414246778 21610 34-35 0.41265484893103377 21610 36-37 0.3275804032328544 21610 38-39 0.20247940766371642 21610 40-41 0.867494473686186 21610 42-43 1.0914886455511272 21610 44-45 0.6349770303911626 21610 46-47 0.6907051690813546 21610 48-49 1.0243743686877664 21610 50-51 0.8438222121715917 21610 52-53 0.37812480741727583 21610 54-55 0.4810922815708949 21610 56-57 1.2425066409512304 21610 58-59 1.2843547387539012 21610 60-61 1.014006370033762 21610 62-63 0.25528142883770144 21610 64-65 0.92291917462207 21610 66-67 0.07192406607022406 21610 68-69 0.7777659228637681 21610 70-71 1.078189178077949 21610 72-73 0.9458044141248649 21610 74-75 1.2850488348506985 21610 76 0.9781771939420167 21611 1 1.0253570505394656 21611 2 0.6683039314610362 21611 3 0.7973600466528623 21611 4 0.669832441959322 21611 5 1.1109311569936544 21611 6 0.8823790466496142 21611 7 0.8034310043144295 21611 8 0.7743544909273545 21611 9 0.20982897726452876 21611 10-11 0.6600331953343499 21611 12-13 0.21553952831665413 21611 14-15 0.4522736517324688 21611 16-17 0.4127817197514041 21611 18-19 0.9902011998294569 21611 20-21 0.23619176659028085 21611 22-23 0.8546297006990926 21611 24-25 0.45886051467932276 21611 26-27 0.5130966369527883 21611 28-29 0.36983844565339297 21611 30-31 0.2237521977948873 21611 32-33 0.0446216743676473 21611 34-35 0.7172711836538994 21611 36-37 0.2898005668888679 21611 38-39 0.24467486852522313 21611 40-41 0.347956107158474 21611 42-43 0.6339002558645426 21611 44-45 0.2993895670593787 21611 46-47 0.3482858391446584 21611 48-49 0.02375679882517545 21611 50-51 0.9233960889664026 21611 52-53 0.6707216886894685 21611 54-55 -0.0028431823784629273 21611 56-57 0.5364467366745593 21611 58-59 0.48369085115161425 21611 60-61 0.30736749401091146 21611 62-63 0.7848784645023663 21611 64-65 0.685926739852885 21611 66-67 0.5714068513103072 21611 68-69 0.5791631746778805 21611 70-71 0.2642243795601189 21611 72-73 0.4826038583119896 21611 74-75 0.38949533786134793 21611 76 0.0871473961961442 21612 1 0.30893843490043693 21612 2 0.5178196413594556 21612 3 1.2595381709146167 21612 4 0.6008978801828029 21612 5 0.9534069424885772 21612 6 0.2561092616271736 21612 7 1.2921151526725616 21612 8 1.12464978976978 21612 9 0.7693848478055187 21612 10-11 0.8417246671037759 21612 12-13 -0.08871277501432218 21612 14-15 0.16767643935354215 21612 16-17 0.2633605054825665 21612 18-19 -0.08806480536780725 21612 20-21 0.004842841478073012 21612 22-23 0.33521084882099217 21612 24-25 0.9083289767629523 21612 26-27 0.5826217964141591 21612 28-29 0.5489312876094488 21612 30-31 0.7513685455388099 21612 32-33 0.2231002947314522 21612 34-35 0.44524189000873804 21612 36-37 -0.021137302234681243 21612 38-39 0.5466592767463432 21612 40-41 1.2064961496815094 21612 42-43 0.6012283919382497 21612 44-45 0.70475219403788 21612 46-47 1.0544989267893534 21612 48-49 0.8427976681849678 21612 50-51 0.9986146101098008 21612 52-53 1.4541849535134688 21612 54-55 1.2522477696650043 21612 56-57 1.3036567532112926 21612 58-59 0.6050232395286557 21612 60-61 1.252819891837511 21612 62-63 1.139874920916256 21612 64-65 1.2247880675164815 21612 66-67 0.6689499649413015 21612 68-69 0.6963141314461296 21612 70-71 1.1035109375568055 21612 72-73 0.843718906740996 21612 74-75 0.9430034881094009 21612 76 0.9021249534841225 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 9.0 16 16.0 17 53.0 18 122.0 19 324.0 20 695.0 21 1481.0 22 2885.0 23 4652.0 24 7409.0 25 10373.0 26 13578.0 27 16833.0 28 20673.0 29 24905.0 30 30206.0 31 36745.0 32 44427.0 33 50539.0 34 42733.0 35 14812.0 36 551.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.85292347576501 15.412249432896624 13.639800626514203 34.09502646482416 2 21.863841317947404 15.173492912452666 34.253855483783354 28.708810285816572 3 20.469352295067296 18.320417503803764 30.858802361575332 30.351427839553608 4 14.207412482524282 28.38550587770546 25.20515645590872 32.20192518386154 5 16.664352001876424 31.07792396171853 28.488894238336403 23.768829798068644 6 30.604497856620405 39.61348184222628 13.971625295891316 15.810395005262004 7 27.927819493181 22.363673959403865 13.86144725187565 35.84705929553949 8 24.66043867527105 39.983519586693454 11.94644791541289 23.409593822622607 9 21.900432379382817 15.86872455797618 11.731647022878148 50.49919603976285 10-11 25.39727363349906 26.71910153971502 23.272411356054082 24.611213470731837 12-13 24.264317833213482 15.455575136025972 26.422833228915586 33.857273801844954 14-15 23.069528008258736 14.746906445734142 15.875230116612993 46.30833542939413 16-17 34.196240367136696 24.462303369226067 14.107264652599676 27.234191611037556 18-19 34.957872970804274 19.192488118017405 14.535985432997963 31.31365347818036 20-21 24.587140956913288 16.6811718993522 13.997240919570029 44.73444622416448 22-23 23.823610198104443 27.027260578789647 14.221454782251769 34.92767444085414 24-25 34.22746400304919 25.79126937956086 14.248326880552312 25.73293973683764 26-27 34.91934164760926 27.897111606963747 14.555846688949172 22.62770005647782 28-29 24.040571444443415 28.226102629150578 14.884837711136006 32.84848821527 30-31 23.382934104477958 28.082173813076643 14.967571496247922 33.56732058619748 32-33 23.22827841405341 38.44365025723641 15.253640967715054 23.07443036099512 34-35 33.48409586460075 20.756003882482382 23.836374045389245 21.923526207527633 36-37 34.246854370550054 24.425268732582147 18.84785245400761 22.480024442860184 38-39 23.21145851657763 19.65196700213875 26.40137521950738 30.73519926177624 40-41 21.837010564130104 17.16678857234562 27.567657651818863 33.42854321170542 42-43 31.711073321132176 15.900750895797392 26.866977559542367 25.521198223528067 44-45 21.872420013672482 26.678621085557943 28.94152468222284 22.507434218546734 46-47 30.513212754757873 17.405270900449352 21.599330679308018 30.482185665484756 48-49 29.07126656136564 16.454752210626758 18.61944486904536 35.85453635896225 50-51 21.769349168880773 17.681912986826525 28.515409708510038 32.03332813578267 52-53 18.371509207909785 18.88964712275978 37.06523350172301 25.673610167607425 54-55 18.46448561487569 18.867682789408203 29.870242134413466 32.79758946130264 56-57 28.208491777371727 17.624944441700823 21.028198923403625 33.138364857523825 58-59 19.60488667615276 18.93833935731196 34.716790695808115 26.73998327072717 60-61 28.722287894173164 26.19035126434795 20.84669491760828 24.240665923870605 62-63 19.26094296357335 39.82396202715256 16.02303554399252 24.892059465281573 64-65 18.45158184191765 41.598075433382405 15.186207066825915 24.764135657874025 66-67 19.112032862067583 41.971970952499994 14.521435339067528 24.394560846364897 68-69 19.14320368124288 42.361914814163285 14.361106224596554 24.133775279997284 70-71 22.14177476151237 38.760913644486 14.234262594091124 24.8630489999105 72-73 38.963986902083505 22.028819119748412 14.210035769286558 24.797158208881523 74-75 42.74359184063971 19.40309332279902 13.948345860832879 23.904968975728387 76 43.78851987988433 19.07931893303212 13.885519765694198 23.246641421389356 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 0.5 7 1.0 8 2.0 9 3.0 10 2.5 11 5.0 12 9.0 13 10.5 14 16.0 15 21.0 16 28.0 17 34.0 18 52.0 19 77.0 20 116.0 21 148.0 22 171.0 23 226.0 24 387.5 25 517.0 26 584.0 27 749.0 28 1094.5 29 1342.0 30 1463.0 31 1730.5 32 2178.5 33 2480.0 34 2860.0 35 3569.5 36 4297.0 37 4695.0 38 5129.5 39 6122.0 40 7407.5 41 9184.5 42 10234.0 43 11666.0 44 16307.5 45 27582.5 46 35648.0 47 29767.0 48 16296.5 49 7854.0 50 7001.0 51 6715.0 52 6180.0 53 5950.0 54 5969.0 55 5976.0 56 6115.0 57 6412.5 58 6578.0 59 6768.5 60 7182.0 61 7430.5 62 7456.0 63 7622.5 64 7770.5 65 7779.0 66 7822.5 67 7839.0 68 7641.5 69 7280.5 70 7158.0 71 7199.0 72 7125.0 73 6979.5 74 6759.5 75 6611.0 76 6384.5 77 5658.5 78 4724.0 79 4289.0 80 3889.0 81 3222.5 82 2546.5 83 2137.0 84 1820.5 85 1267.0 86 825.0 87 620.0 88 494.0 89 289.5 90 185.5 91 122.0 92 84.0 93 83.5 94 109.5 95 146.0 96 156.0 97 203.0 98 375.0 99 1148.5 100 1797.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0018517318321960613 2 6.172439440653538E-4 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 6.172439440653538E-4 14-15 1.5431098601633846E-4 16-17 0.0 18-19 3.086219720326769E-4 20-21 0.0 22-23 0.0 24-25 1.5431098601633846E-4 26-27 0.0 28-29 0.0 30-31 1.5431098601633846E-4 32-33 0.0 34-35 4.6293295804901533E-4 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0024689757762614154 44-45 0.003857774650408461 46-47 0.034102727909610796 48-49 0.05786661975612691 50-51 0.046447606790917875 52-53 0.05215711327352239 54-55 0.03394841692359446 56-57 0.011419012965209044 58-59 0.011727634937241722 60-61 0.006172439440653538 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 1.5431098601633846E-4 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 324021.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.86309461180632 #Duplication Level Percentage of deduplicated Percentage of total 1 79.40983238380915 45.15488811948221 2 9.327187300108108 10.607454678161712 3 4.935023266001392 8.418620846583076 4 2.8482154652487863 6.478333819010067 5 1.5540780361884736 4.418484320295766 6 0.8185114208683097 2.792585541940723 7 0.4239278029894834 1.6874092738976327 8 0.21420031404380868 0.9744074178681376 9 0.12800033975128283 0.6550645886658493 >10 0.2970860950248543 2.718923561608354 >50 0.01821801930311253 0.7150984253014103 >100 0.020361315691713983 2.3643646273280527 >500 0.0021432963886014716 0.8031527264995808 >1k 0.0021432963886014716 3.2543529103725426 >5k 5.358240971503679E-4 1.7510313805740103 >10k+ 5.358240971503679E-4 7.205827762410882 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT 23650 7.298909638572809 TruSeq Adapter, Index 5 (95% over 23bp) TCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG 5747 1.7736504732717941 TruSeq Adapter, Index 5 (95% over 22bp) CTTAAACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT 4848 1.4961993204144175 RNA PCR Primer, Index 5 (95% over 23bp) GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 2362 0.7289650979411828 No Hit CTCTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC 2141 0.6607596421219613 RNA PCR Primer, Index 5 (95% over 21bp) CTAATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT 1330 0.41046722280346026 TruSeq Adapter, Index 5 (95% over 23bp) CTAAAACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCGT 742 0.22899750324824625 No Hit TCTTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCC 738 0.22776301536011553 RNA PCR Primer, Index 5 (95% over 21bp) CTTATACACATCTCCGCGCCCACGAGACACAAAGTGATCTCGTATGCCGT 594 0.18332145138741007 TruSeq Adapter, Index 5 (95% over 23bp) TCATATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGCCG 562 0.17344554828236441 TruSeq Adapter, Index 5 (95% over 22bp) CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCTCGTATGACGT 468 0.14443508291129278 No Hit CTTATACACATCTCCGAGCCCACGAGACACAAAGTGATCACGTATGCCGT 404 0.12468327670120147 No Hit CTTATACACATCTCCGAGCCCACGAGACACAAAGAGATCTCGTATGCCGT 364 0.11233839781989438 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 3.0862197203267687E-4 0.0 2 0.0 0.0 0.0 0.0012344878881307075 0.0 3 0.0 0.0 0.0 0.0012344878881307075 0.0 4 0.0 0.0 0.0 0.002468975776261415 0.0 5 0.0 0.0 0.0 0.005863817468620861 0.0 6 0.0 0.0 0.0 0.005863817468620861 0.0 7 0.0 0.0 0.0 0.006172439440653538 0.0 8 0.0 0.0 0.0 0.006172439440653538 0.0 9 0.0 0.0 0.0 0.010493147049111015 3.0862197203267687E-4 10 0.0 0.0 0.0 0.014505232685535814 3.0862197203267687E-4 11 0.0 0.0 0.0 0.016048342545699198 3.0862197203267687E-4 12 0.0 0.0 0.0 0.01975180621009132 3.0862197203267687E-4 13 0.0 0.0 0.0 0.020060428182123998 3.0862197203267687E-4 14 0.0 0.0 0.0 0.02191216001432006 3.0862197203267687E-4 15 0.0 0.0 0.0 0.027467355510908245 3.0862197203267687E-4 16 0.0 0.0 0.0 0.02962770931513698 3.0862197203267687E-4 17 0.0 0.0 0.0 0.02962770931513698 3.0862197203267687E-4 18 0.0 0.0 0.0 0.03271392903546375 3.0862197203267687E-4 19 0.0 0.0 0.0 0.03981223439221532 3.0862197203267687E-4 20 0.0 0.0 0.0 0.04289845411254209 3.0862197203267687E-4 21 0.0 0.0 0.0 0.04660191777693421 3.0862197203267687E-4 22 0.0 0.0 0.0 0.05246573524555507 3.0862197203267687E-4 23 0.0 0.0 0.0 0.05524333299384916 3.0862197203267687E-4 24 0.0 0.0 0.0 0.05832955271417593 3.0862197203267687E-4 25 0.0 0.0 0.0 0.06388474821076412 3.0862197203267687E-4 26 0.0 0.0 0.0 0.07098305356751569 3.0862197203267687E-4 27 0.0 0.0 0.0 0.14381783896722744 3.0862197203267687E-4 28 0.0 0.0 0.0 0.231157857052475 3.0862197203267687E-4 29 0.0 0.0 0.0 0.3188064971097552 3.0862197203267687E-4 30 0.0 0.0 0.0 0.392567148425565 3.0862197203267687E-4 31 0.0 0.0 0.0 0.49626413102854444 3.0862197203267687E-4 32 0.0 0.0 0.0 0.6258853592822687 3.0862197203267687E-4 33 0.0 0.0 0.0 0.8129102743340709 3.0862197203267687E-4 34 0.0 0.0 0.0 1.065054425484768 3.0862197203267687E-4 35 0.0 0.0 0.0 1.4286111085392614 3.0862197203267687E-4 36 0.0 0.0 0.0 1.9711685353727073 3.0862197203267687E-4 37 0.0 0.0 0.0 2.7501303927831837 3.0862197203267687E-4 38 0.0 0.0 0.0 3.471071319451517 3.0862197203267687E-4 39 0.0 0.0 0.0 4.235836566148491 3.0862197203267687E-4 40 0.0 0.0 0.0 5.018810509195392 3.0862197203267687E-4 41 0.0 0.0 0.0 5.876162347502168 3.0862197203267687E-4 42 0.0 0.0 0.0 6.734440051725042 3.0862197203267687E-4 43 0.0 0.0 0.0 7.615555781878335 3.0862197203267687E-4 44 0.0 0.0 0.0 8.46364896102413 3.0862197203267687E-4 45 0.0 0.0 0.0 9.39753904839501 3.0862197203267687E-4 46 0.0 0.0 0.0 10.257977106422114 3.0862197203267687E-4 47 0.0 0.0 0.0 11.080454661889199 3.0862197203267687E-4 48 0.0 0.0 0.0 11.839973335061616 3.0862197203267687E-4 49 0.0 0.0 0.0 12.550421114680839 3.0862197203267687E-4 50 0.0 0.0 0.0 13.16272710719367 3.0862197203267687E-4 51 0.0 0.0 0.0 13.819783285651239 3.0862197203267687E-4 52 0.0 0.0 0.0 14.492887806654506 3.0862197203267687E-4 53 0.0 0.0 0.0 15.224939124316016 3.0862197203267687E-4 54 0.0 0.0 0.0 15.912857499976854 3.0862197203267687E-4 55 0.0 0.0 0.0 16.539977347147254 3.0862197203267687E-4 56 0.0 0.0 0.0 17.125741850065275 3.0862197203267687E-4 57 0.0 0.0 0.0 17.682495887612223 3.0862197203267687E-4 58 0.0 0.0 0.0 18.315788174223275 3.0862197203267687E-4 59 0.0 0.0 0.0 18.82007647652467 3.0862197203267687E-4 60 0.0 0.0 0.0 19.293502581622796 3.0862197203267687E-4 61 0.0 0.0 0.0 19.756744161643844 3.0862197203267687E-4 62 0.0 0.0 0.0 20.253316914644422 3.0862197203267687E-4 63 0.0 0.0 0.0 20.746803447924673 3.0862197203267687E-4 64 0.0 0.0 0.0 21.278250483764943 3.0862197203267687E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGGTA 15 0.002226163 69.97855 12 AGGGGGG 2135 0.0 59.32608 70 ACGAAAG 30 8.358804E-6 58.342464 40 AACACAT 1745 0.0 57.346317 5 AAACACA 1860 0.0 56.998653 4 ACGATAC 25 2.3920856E-4 55.98284 22 CTTAAAC 1110 0.0 55.793713 1 CTAAAAC 505 0.0 55.428555 1 TTAAACA 1280 0.0 53.85068 2 TAAACAC 1335 0.0 53.728848 3 AAAACAC 670 0.0 52.745026 3 TAAGCCG 280 0.0 52.548786 43 GCGTACC 20 0.0069559803 52.483913 11 TAAAACA 605 0.0 52.050163 2 CACGTAT 360 0.0 51.535843 39 ATTAAAC 145 0.0 50.674126 1 AAGCCGT 300 0.0 50.213287 44 GTAAGCC 290 0.0 49.528744 42 CGTAAGC 305 0.0 49.390118 41 TAAGACG 50 6.137998E-8 49.045532 43 >>END_MODULE