FastQCFastQC Report
Wed 25 May 2016
SRR1781218_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781218_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences308774
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTCTA452.6950147E-854.4444431
AATGCGT200.006947246452.58
AGCCTTA552.2773747E-950.909096
CTCAACG551.3084355E-744.54545246
CAACGGC551.3084355E-744.54545248
GGTGCAA350.001254342840.01
CGAATTC459.186271E-538.88888514
CGCTTGT459.186271E-538.8888858
CAAGTGA601.2136721E-535.028
TGCTACC601.2136721E-535.038
ACGTAAG400.00241670335.029
GCCTTAC1055.475158E-1033.3333367
CCTAACG552.988423E-431.8181820
CTGGTCA552.988423E-431.8181817
GCTCTAG552.988423E-431.818181
GTTCGAA552.988423E-431.8181811
CTGAATA450.0043036331.11110919
GTAGCGA450.0043036331.11110922
TATCGTG450.0043036331.11110942
GCGCTTG802.4608944E-630.6257