FastQCFastQC Report
Wed 25 May 2016
SRR1781218_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781218_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences278821
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT47121.6899731368871067No Hit
TCTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG4940.17717460306074506No Hit
CTTATACACATCTCCGAGCCCACGAGACAAATACAGACCTCGTATGCCGT3920.14059199271217018No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCC2890.1036507293209622No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTAT352.9677358E-760.00000439
ATATCGT252.3878862E-456.00000437
TCGTATG7550.055.1655640
CCGTCTT7300.055.13698647
CTCGTAT7050.055.1063839
TATGCCG7500.055.0666643
TCTTCTG7450.054.96644250
TCTCGTA5900.054.5762738
CGTCTTC7400.054.3918948
CGTATGC7700.054.09090841
GTCTTCT7700.053.18181649
CTTGAAA7250.053.1034557
GGATCCA200.00694654352.562
ACACTGC200.00694654352.56
GCCGTCT7750.052.38709346
ATGCCGT7950.051.94968444
AGATCTC6300.051.66666435
GTATGCC8000.051.62500442
CTTCTGC7750.051.48386851
ACAGATC6300.051.1111133
Produced by FastQC (version 0.11.5)