##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781218_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	278821
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.24693620638331	34.0	34.0	34.0	32.0	34.0
2	33.28152470581484	34.0	34.0	34.0	33.0	34.0
3	33.32124194375603	34.0	34.0	34.0	33.0	34.0
4	33.362917427309995	34.0	34.0	34.0	33.0	34.0
5	33.396110766405684	34.0	34.0	34.0	33.0	34.0
6	36.815404865487174	38.0	37.0	38.0	34.0	38.0
7	36.709010440390074	38.0	37.0	38.0	34.0	38.0
8	36.49146943738097	38.0	37.0	38.0	34.0	38.0
9	36.43780059608136	38.0	37.0	38.0	34.0	38.0
10-11	36.35182428870135	38.0	37.0	38.0	34.0	38.0
12-13	36.29042109453735	38.0	37.0	38.0	34.0	38.0
14-15	36.2990484934779	38.0	37.0	38.0	34.0	38.0
16-17	36.23137425086345	38.0	37.0	38.0	34.0	38.0
18-19	36.233201588115676	38.0	37.0	38.0	34.0	38.0
20-21	36.174954540726844	38.0	37.0	38.0	33.5	38.0
22-23	36.23253628672158	38.0	37.0	38.0	34.0	38.0
24-25	36.232290609387384	38.0	37.0	38.0	34.0	38.0
26-27	36.00602178458581	38.0	36.5	38.0	32.0	38.0
28-29	36.00619214478106	38.0	37.0	38.0	32.0	38.0
30-31	36.03933168592036	38.0	37.0	38.0	32.0	38.0
32-33	35.98980887379358	38.0	37.0	38.0	32.0	38.0
34-35	35.97986163165615	38.0	37.0	38.0	32.0	38.0
36-37	35.90884115615395	38.0	36.5	38.0	31.5	38.0
38-39	35.74570423318187	38.0	36.0	38.0	31.0	38.0
40-41	35.86533474881735	38.0	36.0	38.0	31.5	38.0
42-43	35.921704247527984	38.0	36.5	38.0	32.0	38.0
44-45	35.88186148102187	38.0	36.0	38.0	31.5	38.0
46-47	35.90026576190459	38.0	36.5	38.0	32.0	38.0
48-49	35.877708278788184	38.0	36.0	38.0	31.5	38.0
50-51	35.78693857349339	38.0	36.0	38.0	31.0	38.0
52-53	35.814289454524584	38.0	36.0	38.0	31.0	38.0
54-55	35.83435609225991	38.0	36.0	38.0	31.5	38.0
56-57	35.77463856739628	38.0	36.0	38.0	31.0	38.0
58-59	35.76250533496401	38.0	36.0	38.0	31.0	38.0
60-61	35.75072537577873	38.0	36.0	38.0	31.0	38.0
62-63	35.73905121924102	38.0	36.0	38.0	31.0	38.0
64-65	35.692471155329045	38.0	36.0	38.0	31.0	38.0
66-67	35.64221310446487	38.0	36.0	38.0	31.0	38.0
68-69	35.54413942995686	38.0	36.0	38.0	31.0	38.0
70-71	35.40942934714387	38.0	36.0	38.0	30.0	38.0
72-73	35.28634141617741	38.0	36.0	38.0	29.0	38.0
74-75	35.30391541526643	38.0	36.0	38.0	29.0	38.0
76	34.373182077390155	37.0	34.0	38.0	25.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	4.0
21	12.0
22	37.0
23	93.0
24	196.0
25	414.0
26	855.0
27	1433.0
28	2477.0
29	3992.0
30	6157.0
31	8791.0
32	12393.0
33	17271.0
34	23444.0
35	32247.0
36	47619.0
37	121386.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.44830554370008	18.265123502175232	10.239544367174638	28.047026586950054
2	23.664286405973726	19.796930647261146	31.409757514677878	25.129025432087253
3	19.96262835295763	21.89218172232364	29.091424247097603	29.053765677621122
4	14.033734905190068	17.8236216066939	35.743003575770835	32.399639912345194
5	13.698752963370763	33.96731236169442	36.173745879973175	16.16018879496164
6	30.007424117982506	38.25106430290401	17.054669483288563	14.68684209582492
7	29.22018068940288	28.435806485164317	21.185276575293827	21.15873625013898
8	25.782132622722102	35.35745155494027	18.511518142464162	20.348897679873467
9	24.44794330412702	15.950018111978654	19.74743652737778	39.85460205651655
10-11	25.233214140972166	24.897514893067594	26.877459014923556	22.991811951036688
12-13	24.898052872631546	22.673507375699824	26.225248456895283	26.203191294773347
14-15	23.03323637746081	23.283038221654753	22.77375090111577	30.909974499768666
16-17	24.97713586853214	26.969094867316308	24.036568264226872	24.017200999924682
18-19	24.441487549359625	25.735328400658485	25.023043457989175	24.80014059199271
20-21	22.924743832064298	25.764737950154398	24.900204790887344	26.410313426893957
22-23	23.442818152147794	26.453710445052558	25.430293987899045	24.6731774149006
24-25	24.319007535300425	26.59125388690235	25.217792060138944	23.871946517658284
26-27	23.509168965034917	27.19181840679145	25.502383249468295	23.796629378705333
28-29	23.125230882896194	26.655452781533672	26.140785665355192	24.07853067021494
30-31	22.8542685091869	27.905358635109984	25.65732136388579	23.58305149181733
32-33	22.479117426592687	27.433550557526154	26.521316543588895	23.56601547229226
34-35	23.48908439464746	26.362791898745076	25.564968205407773	24.583155501199695
36-37	24.63516019238149	26.17826490831035	25.155207104199466	24.03136779510869
38-39	22.69843376216282	25.87197520990169	26.85504319975899	24.5745478281765
40-41	22.875070385659615	24.492236954892206	27.724597501622906	24.908095157825272
42-43	23.767040502688104	24.698821107448865	27.339583460356288	24.194554929506744
44-45	21.882139437129915	26.039645507332658	27.691960074743292	24.38625498079413
46-47	23.432237887390116	24.089648914536564	26.968736214273676	25.50937698379964
48-49	22.725153413838985	23.973266002202127	27.506895104744622	25.794685479214262
50-51	21.31582628281227	24.243152416783527	28.61567098604481	25.82535031435939
52-53	20.82769949178864	24.221274581182907	30.144429580268344	24.806596346760106
54-55	20.03328300235635	24.508914321374643	29.317375663956447	26.14042701231256
56-57	20.900506059443156	24.805161734589575	27.89764759469337	26.3966846112739
58-59	20.29276847870139	24.49672011792512	29.328314581756754	25.882196821616738
60-61	20.981561647078234	25.474766965185548	27.452917821828343	26.090753565907875
62-63	19.056670767266453	26.667646984983197	27.153263204708395	27.12241904304195
64-65	19.653469430207913	27.2454370366651	26.071206975084372	27.029886558042616
66-67	19.893049662686813	27.012312558953592	25.57339655190965	27.521241226449945
68-69	19.683775612310406	27.439289006208284	25.07271690439386	27.80421847708745
70-71	20.298686253904837	27.062882637964858	24.8173559380391	27.821075170091202
72-73	19.947385598645724	26.429142711632192	24.754233002535678	28.869238687186403
74-75	19.73972548696117	26.985234254234797	24.605391989842946	28.66964826896109
76	20.271787275707354	27.235394751471375	24.276507149748404	28.216310823072867
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.5
12	1.0
13	0.5
14	2.0
15	5.5
16	7.5
17	8.0
18	13.0
19	26.0
20	45.0
21	56.0
22	74.0
23	153.0
24	319.5
25	425.0
26	571.0
27	779.5
28	1099.5
29	1357.0
30	1545.0
31	1987.0
32	3042.0
33	3843.0
34	4260.0
35	5511.0
36	7092.5
37	7840.0
38	9135.0
39	12395.0
40	15693.0
41	17602.0
42	18178.0
43	18399.5
44	18367.5
45	18262.0
46	18410.0
47	18203.5
48	16900.0
49	15196.5
50	14590.0
51	13391.0
52	10882.5
53	8777.0
54	7981.0
55	7180.5
56	5764.0
57	4562.5
58	3977.0
59	3656.5
60	3094.0
61	2753.0
62	2654.0
63	2521.5
64	2409.5
65	2504.0
66	2561.5
67	2545.0
68	2511.5
69	2459.0
70	2447.0
71	2454.0
72	2392.5
73	2226.0
74	2072.5
75	2024.0
76	1888.5
77	1594.0
78	1286.0
79	1137.0
80	996.5
81	721.0
82	540.5
83	495.0
84	374.0
85	221.5
86	156.5
87	123.0
88	92.0
89	47.0
90	25.0
91	12.0
92	7.0
93	7.0
94	3.5
95	0.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	278821.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.15065938361888
#Duplication Level	Percentage of deduplicated	Percentage of total
1	58.85146758122275	19.509649560112045
2	18.22007767956638	12.080151781967642
3	7.8101502742586355	7.76734894430477
4	3.6892384589585743	4.892027501515309
5	2.019885103482598	3.3480261529798687
6	1.310166502580303	2.6059730077720116
7	1.0277937055749693	2.38504273351003
8	0.8341357336824226	2.2121719669608817
9	0.6383139855676125	1.9044476563816928
>10	5.277450206099686	33.69509470233591
>50	0.2910279018943861	6.367526118907829
>100	0.029210979000551765	1.542566736364908
>500	0.0	0.0
>1k	0.0010818881111315467	1.6899731368871067
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCGT	4712	1.6899731368871067	No Hit
TCTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCCG	494	0.17717460306074506	No Hit
CTTATACACATCTCCGAGCCCACGAGACAAATACAGACCTCGTATGCCGT	392	0.14059199271217018	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAAATACAGATCTCGTATGCC	289	0.1036507293209622	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0010759591278992615	0.0
2	0.0	0.0	0.0	0.0010759591278992615	0.0
3	0.0	0.0	0.0	0.0010759591278992615	0.0
4	0.0	0.0	0.0	0.0014346121705323487	0.0
5	0.0	0.0	0.0	0.0028692243410646974	0.0
6	0.0	0.0	0.0	0.0028692243410646974	0.0
7	0.0	0.0	0.0	0.0028692243410646974	0.0
8	0.0	0.0	0.0	0.0028692243410646974	0.0
9	0.0	0.0	0.0	0.0028692243410646974	0.0
10	0.0	0.0	0.0	0.0028692243410646974	0.0
11	0.0	0.0	0.0	0.0028692243410646974	0.0
12	0.0	0.0	0.0	0.0028692243410646974	0.0
13	0.0	0.0	0.0	0.0028692243410646974	0.0
14	0.0	0.0	0.0	0.0028692243410646974	0.0
15	0.0	0.0	0.0	0.0032278773836977846	0.0
16	0.0	0.0	0.0	0.0032278773836977846	0.0
17	0.0	0.0	0.0	0.0032278773836977846	0.0
18	0.0	0.0	0.0	0.003945183468963959	0.0
19	0.0	0.0	0.0	0.004303836511597046	0.0
20	0.0	0.0	0.0	0.004662489554230134	0.0
21	0.0	0.0	0.0	0.004662489554230134	0.0
22	0.0	0.0	0.0	0.005379795639496308	0.0
23	0.0	0.0	0.0	0.006097101724762482	0.0
24	0.0	0.0	0.0	0.0071730608526617435	0.0
25	0.0	0.0	0.0	0.0071730608526617435	0.0
26	0.0	0.0	0.0	0.007890366937927919	0.0
27	0.0	0.0	0.0	0.019725917344819793	0.0
28	0.0	0.0	0.0	0.0390931816470065	0.0
29	0.0	0.0	0.0	0.060612364204991734	0.0
30	0.0	0.0	0.0	0.07782771025137991	0.0
31	0.0	0.0	0.0	0.0979122806388328	0.0
32	0.0	0.0	0.0	0.12337664666578199	0.0
33	0.0	0.0	0.0	0.1678496239522848	0.0
34	0.0	0.0	0.0	0.2435254159478662	0.0
35	0.0	0.0	0.0	0.3611636139315188	0.0
36	0.0	0.0	0.0	0.5505324204417889	0.0
37	0.0	0.0	0.0	0.847497139741985	0.0
38	0.0	0.0	0.0	1.2567202613863375	0.0
39	0.0	0.0	0.0	1.7251211350651494	0.0
40	0.0	0.0	0.0	2.2469613120962912	0.0
41	0.0	0.0	0.0	2.841966709824583	0.0
42	0.0	0.0	0.0	3.5029642673973624	0.0
43	0.0	0.0	0.0	4.252549126500515	0.0
44	0.0	0.0	0.0	5.085700144537176	0.0
45	0.0	0.0	0.0	6.078093113502929	0.0
46	0.0	0.0	0.0	7.082321632875573	0.0
47	0.0	0.0	0.0	8.118470273042561	0.0
48	0.0	0.0	0.0	9.127002628926803	0.0
49	0.0	0.0	0.0	10.12943788308628	0.0
50	0.0	0.0	0.0	11.04113391745959	0.0
51	0.0	0.0	0.0	11.871415711155185	0.0
52	0.0	0.0	0.0	12.729672442176163	0.0
53	0.0	0.0	0.0	13.586135907983975	0.0
54	0.0	0.0	0.0	14.497114636272016	0.0
55	0.0	0.0	0.0	15.492735482621466	0.0
56	0.0	0.0	0.0	16.447469882110745	0.0
57	0.0	0.0	0.0	17.386423547724167	0.0
58	0.0	0.0	0.0	18.328246437678654	0.0
59	0.0	0.0	0.0	19.20694639212972	0.0
60	0.0	0.0	0.0	20.066279082278594	0.0
61	0.0	0.0	0.0	20.918080058532176	0.0
62	0.0	0.0	0.0	21.72146287403029	0.0
63	0.0	0.0	0.0	22.553537932939054	0.0
64	0.0	0.0	0.0	23.42434752045219	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGTAT	35	2.9677358E-7	60.000004	39
ATATCGT	25	2.3878862E-4	56.000004	37
TCGTATG	755	0.0	55.16556	40
CCGTCTT	730	0.0	55.136986	47
CTCGTAT	705	0.0	55.10638	39
TATGCCG	750	0.0	55.06666	43
TCTTCTG	745	0.0	54.966442	50
TCTCGTA	590	0.0	54.57627	38
CGTCTTC	740	0.0	54.39189	48
CGTATGC	770	0.0	54.090908	41
GTCTTCT	770	0.0	53.181816	49
CTTGAAA	725	0.0	53.10345	57
GGATCCA	20	0.006946543	52.5	62
ACACTGC	20	0.006946543	52.5	6
GCCGTCT	775	0.0	52.387093	46
ATGCCGT	795	0.0	51.949684	44
AGATCTC	630	0.0	51.666664	35
GTATGCC	800	0.0	51.625004	42
CTTCTGC	775	0.0	51.483868	51
ACAGATC	630	0.0	51.11111	33
>>END_MODULE