FastQCFastQC Report
Wed 25 May 2016
SRR1781217_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781217_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences229834
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGAAT200.006944997252.566
GTTAAGC350.001253673840.021
CGGTATG350.001253673840.043
CGTCCAA350.001253673840.040
ACTAATG459.178936E-538.88888513
CAAGTGC400.002415421235.044
GCGGTAT400.002415421235.042
GACGCTC400.002415421235.058
CTTTAGA400.002415421235.02
GCACTTT806.2140316E-835.049
AGAGATC501.7062368E-435.06
CTTACGT400.002415421235.023
AAGAGAT552.9860612E-431.818185
ACTTATG552.9860612E-431.8181848
TACGGAC552.9860612E-431.8181838
GCATAAG450.00430136131.11110952
ATCTCAG450.00430136131.11110952
AAGCGCG450.00430136131.1111097
GAATAAG450.00430136131.11110936
TTATCTT450.00430136131.11110932