##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781217_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	229834
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.334154215651296	34.0	32.0	34.0	23.0	34.0
2	31.21841415978489	34.0	32.0	34.0	23.0	34.0
3	31.200897169261292	34.0	32.0	34.0	23.0	34.0
4	31.370824160045945	34.0	33.0	34.0	23.0	34.0
5	31.37300834515346	34.0	33.0	34.0	23.0	34.0
6	34.14634475316968	37.0	34.0	38.0	24.0	38.0
7	34.04895272239965	37.0	34.0	38.0	24.0	38.0
8	33.86892713871751	37.0	34.0	38.0	23.0	38.0
9	34.19443163326575	37.0	34.0	38.0	24.0	38.0
10-11	34.018206618690016	37.0	34.0	38.0	23.0	38.0
12-13	33.984332170174994	37.0	34.0	38.0	23.0	38.0
14-15	33.84370241130555	37.0	34.0	38.0	23.0	38.0
16-17	33.82755597518209	37.0	34.0	38.0	23.0	38.0
18-19	33.88052681500561	37.0	34.0	38.0	23.0	38.0
20-21	33.78157931376559	37.0	34.0	38.0	22.5	38.0
22-23	33.85866103361556	37.0	34.0	38.0	23.0	38.0
24-25	33.86095399288182	37.0	34.0	38.0	23.0	38.0
26-27	33.77624067805459	37.0	34.0	38.0	23.0	38.0
28-29	33.85470382972058	37.0	34.0	38.0	23.0	38.0
30-31	33.94800812760514	37.0	34.0	38.0	23.0	38.0
32-33	33.889185238041364	37.0	34.0	38.0	23.0	38.0
34-35	33.8921025609788	37.0	34.0	38.0	23.0	38.0
36-37	33.74946700662217	37.0	34.0	38.0	22.5	38.0
38-39	33.76363375305655	37.0	34.0	38.0	23.0	38.0
40-41	33.76562214467833	37.0	34.0	38.0	23.0	38.0
42-43	33.84196637573205	37.0	34.0	38.0	23.0	38.0
44-45	33.87705691934178	37.0	34.0	38.0	23.0	38.0
46-47	33.87491624389777	37.0	34.0	38.0	23.0	38.0
48-49	33.83339714750646	37.0	34.0	38.0	23.0	38.0
50-51	33.69105963434479	37.0	34.0	38.0	22.5	38.0
52-53	33.76421460706423	37.0	34.0	38.0	23.0	38.0
54-55	33.73993403935014	37.0	34.0	38.0	23.0	38.0
56-57	33.70580505930367	37.0	34.0	38.0	22.5	38.0
58-59	33.71215529469095	37.0	34.0	38.0	23.0	38.0
60-61	33.690102421747866	37.0	34.0	38.0	23.0	38.0
62-63	33.657563719902186	37.0	34.0	38.0	22.5	38.0
64-65	33.674597753160974	37.0	34.0	38.0	23.0	38.0
66-67	33.574769181235155	37.0	34.0	38.0	22.0	38.0
68-69	33.33675609352837	37.0	34.0	38.0	21.5	38.0
70-71	33.48203268445922	37.0	34.0	38.0	22.0	38.0
72-73	33.43788777987591	37.0	33.5	38.0	22.0	38.0
74-75	33.43985659214911	37.0	33.0	38.0	22.0	38.0
76	32.01903982874597	36.0	31.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	8.0
13	38.0
14	110.0
15	343.0
16	814.0
17	1491.0
18	1912.0
19	2123.0
20	2039.0
21	1957.0
22	2053.0
23	2412.0
24	2805.0
25	3378.0
26	4126.0
27	4769.0
28	5761.0
29	6853.0
30	8230.0
31	9618.0
32	11594.0
33	14020.0
34	17408.0
35	22821.0
36	33495.0
37	69654.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.75881723330752	17.905966915251877	8.934274302322546	25.400941549118063
2	24.199204643351287	19.64678855173734	30.382798019440116	25.771208785471256
3	19.831704621596458	21.507261762837526	29.465179216303945	29.19585439926208
4	14.184585396416544	16.30220071877964	36.86878355682797	32.64443032797584
5	13.942671667377324	33.64167181531018	35.86196994352446	16.55368657378804
6	29.89766527145679	37.00714428674608	18.424167007492365	14.67102343430476
7	29.335520419085082	28.592375366568916	22.22038514754127	19.851719066804737
8	25.889119973546126	33.531157270029674	19.638086619038088	20.941636137386112
9	24.82226302461777	15.573848951852206	21.048234812951957	38.555653210578065
10-11	25.913920481738995	23.65729178450534	27.609492068188345	22.81929566556732
12-13	25.72378325226033	22.34286485028325	26.201736905766772	25.731614991689653
14-15	24.321684346093267	23.2398165632587	23.547212335859793	28.891286754788236
16-17	24.468747008710633	26.115370223726693	24.947788403804484	24.46809436375819
18-19	23.774115230992805	25.906958935579596	25.92762602574032	24.391299807687286
20-21	23.764325556706144	25.858663209098744	25.99093258612738	24.386078648067734
22-23	24.026471279271124	25.41573483470679	26.415804450168384	24.141989435853702
24-25	23.49652357788665	25.834297797540835	26.190859489892702	24.47831913467981
26-27	22.97136193948676	26.04706005203756	26.378168591244116	24.603409417231568
28-29	23.909647832783662	25.42443676740604	27.264895533297945	23.40101986651235
30-31	23.941409887135933	25.450760113821282	26.456921082172354	24.150908916870435
32-33	23.44518217496106	25.940243828154237	26.1012295830904	24.513344413794304
34-35	24.513126865476824	25.088324616897413	26.47476004420582	23.923788473419947
36-37	24.47135758852041	24.838144051793904	25.938285893296904	24.752212466388784
38-39	23.618133087358704	25.6030439360582	27.000139230923185	23.77868374565991
40-41	23.857653784905626	24.469182105345595	27.37084156391135	24.30232254583743
42-43	23.324877955393895	24.924728282151463	26.9196463534551	24.83074740899954
44-45	22.65939765221856	25.016968768763544	27.316019387906053	25.007614191111845
46-47	23.12386331004116	24.460480172646346	27.45111689306195	24.964539624250545
48-49	22.6402534002802	24.282960745581594	28.155320796748956	24.92146505738925
50-51	22.341342012060878	24.581872133800918	27.790709816650278	25.286076037487927
52-53	22.00675269977462	24.356056980255314	28.359381118546427	25.277809201423633
54-55	21.369553677871856	24.918201832627027	28.383746530104336	25.328497959396785
56-57	21.25042965979646	25.09430760595737	28.134993669316415	25.520269064929753
58-59	21.789686882025467	24.70005460474648	27.785114006443795	25.725144506784257
60-61	21.28732041386392	24.568601686434558	28.000426394702266	26.14365150499926
62-63	20.641246815629575	24.20339071545271	28.628333119410353	26.52702934950736
64-65	21.453791549099673	24.455747312728562	27.782938518536245	26.30752261963552
66-67	21.654589082966584	24.42550803081361	27.078950631652916	26.84095225456689
68-69	21.42072413290491	24.648277992546777	26.79048093511172	27.140516939436594
70-71	21.934744206688308	24.39717361225928	26.876789334911283	26.79129284614113
72-73	22.00327192669492	23.771069554548067	26.77911014036217	27.44654837839484
74-75	22.320457373582673	24.126761053630013	26.46322998337931	27.089551589408007
76	22.776873743658467	23.949894271517707	26.19020684494026	27.08302513988357
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	1.0
12	2.0
13	2.0
14	3.5
15	4.5
16	5.0
17	6.0
18	18.5
19	37.5
20	54.0
21	64.0
22	85.5
23	156.5
24	308.5
25	411.0
26	526.5
27	752.5
28	961.0
29	1059.0
30	1300.0
31	1721.5
32	2562.0
33	3222.0
34	3670.5
35	4707.5
36	6130.5
37	6965.0
38	7668.0
39	9431.5
40	11595.0
41	13460.5
42	14223.0
43	14452.0
44	14885.0
45	15004.0
46	14919.0
47	14660.5
48	13620.5
49	12330.0
50	11821.0
51	10827.5
52	8808.5
53	7126.0
54	6469.0
55	5852.5
56	4779.5
57	3900.5
58	3478.0
59	3139.5
60	2774.0
61	2692.0
62	2637.0
63	2510.0
64	2369.0
65	2302.0
66	2296.5
67	2344.0
68	2314.5
69	2335.5
70	2347.5
71	2309.0
72	2234.0
73	2082.5
74	1925.5
75	1845.0
76	1723.5
77	1461.0
78	1180.5
79	1041.0
80	900.0
81	654.0
82	443.0
83	337.0
84	281.0
85	187.0
86	117.5
87	86.0
88	70.0
89	40.5
90	19.0
91	8.0
92	5.0
93	3.5
94	3.0
95	3.5
96	3.0
97	2.0
98	0.5
99	2.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	4.350966349626252E-4
58-59	2.175483174813126E-4
60-61	0.0
62-63	2.175483174813126E-4
64-65	2.175483174813126E-4
66-67	2.175483174813126E-4
68-69	2.175483174813126E-4
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	229834.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.487750536180506
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.5871654768638	32.29239709863171
2	12.082723490839562	10.75066376916237
3	5.025716140945086	6.707484178320621
4	2.4557002014894955	4.369943118220515
5	1.4132262757876692	3.1435629004208625
6	0.9075020572846357	2.4223635081329675
7	0.7845694469602901	2.443261088427494
8	0.5836796873689779	2.077327705976553
9	0.48073618069381546	1.9248184152380392
>10	3.5340605714987925	29.665947022745563
>50	0.13692485811516847	3.7998378785425055
>100	0.007995612152710569	0.40239331618078256
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0034807730797010014	0.0
2	0.0	0.0	0.0	0.0034807730797010014	0.0
3	0.0	0.0	0.0	0.0034807730797010014	0.0
4	0.0	0.0	0.0	0.004350966349626252	0.0
5	0.0	0.0	0.0	0.004786062984588877	0.0
6	0.0	0.0	0.0	0.004786062984588877	0.0
7	0.0	0.0	0.0	0.005656256254514128	0.0
8	0.0	0.0	0.0	0.005656256254514128	0.0
9	0.0	0.0	0.0	0.006961546159402003	0.0
10	0.0	0.0	0.0	0.006961546159402003	0.0
11	0.0	0.0	0.0	0.006961546159402003	0.0
12	0.0	0.0	0.0	0.006961546159402003	0.0
13	0.0	0.0	0.0	0.006961546159402003	0.0
14	0.0	0.0	0.0	0.007396642794364628	0.0
15	0.0	0.0	0.0	0.008266836064289879	0.0
16	0.0	0.0	0.0	0.008701932699252504	0.0
17	0.0	0.0	0.0	0.008701932699252504	0.0
18	0.0	0.0	0.0	0.00913702933421513	0.0
19	0.0	0.0	0.0	0.01000722260414038	4.3509663496262517E-4
20	0.0	0.0	0.0	0.01000722260414038	4.3509663496262517E-4
21	0.0	0.0	0.0	0.012182705778953506	4.3509663496262517E-4
22	0.0	0.0	0.0	0.013923092318804006	4.3509663496262517E-4
23	0.0	0.0	0.0	0.016098575493617134	4.3509663496262517E-4
24	0.0	0.0	0.0	0.017838962033467635	4.3509663496262517E-4
25	0.0	0.0	0.0	0.01914425193835551	4.3509663496262517E-4
26	0.0	0.0	0.0	0.021319735113168634	4.3509663496262517E-4
27	0.0	0.0	0.0	0.03176205435227164	4.3509663496262517E-4
28	0.0	0.0	0.0	0.04525005003611302	4.3509663496262517E-4
29	0.0	0.0	0.0	0.06526449524439377	4.3509663496262517E-4
30	0.0	0.0	0.0	0.08179816737297353	4.3509663496262517E-4
31	0.0	0.0	0.0	0.10572848229591793	4.3509663496262517E-4
32	0.0	0.0	0.0	0.1327044736636007	4.3509663496262517E-4
33	0.0	0.0	0.0	0.17229826744519958	4.3509663496262517E-4
34	0.0	0.0	0.0	0.2506156617384721	4.3509663496262517E-4
35	0.0	0.0	0.0	0.3615653036539415	4.3509663496262517E-4
36	0.0	0.0	0.0	0.5277722182096644	4.3509663496262517E-4
37	0.0	0.0	0.0	0.7801282664879869	4.3509663496262517E-4
38	0.0	0.0	0.0	1.0873064907716004	4.3509663496262517E-4
39	0.0	0.0	0.0	1.4606194035695328	4.3509663496262517E-4
40	0.0	0.0	0.0	1.838718379352054	4.3509663496262517E-4
41	0.0	0.0	0.0	2.252930375836473	4.3509663496262517E-4
42	0.0	0.0	0.0	2.7411088002645387	4.3509663496262517E-4
43	0.0	0.0	0.0	3.295857009841886	4.3509663496262517E-4
44	0.0	0.0	0.0	3.93457886996702	4.3509663496262517E-4
45	0.0	0.0	0.0	4.721233585979446	4.3509663496262517E-4
46	0.0	0.0	0.0	5.59708311215921	4.3509663496262517E-4
47	0.0	0.0	0.0	6.480764377768303	4.3509663496262517E-4
48	0.0	0.0	0.0	7.308318177467216	4.3509663496262517E-4
49	0.0	0.0	0.0	8.134566687261241	4.3509663496262517E-4
50	0.0	0.0	0.0	8.833331883011217	4.3509663496262517E-4
51	0.0	0.0	0.0	9.521219662887127	4.3509663496262517E-4
52	0.0	0.0	0.0	10.209107442763038	4.3509663496262517E-4
53	0.0	0.0	0.0	10.913528894767527	4.3509663496262517E-4
54	0.0	0.0	0.0	11.695832644430327	4.3509663496262517E-4
55	0.0	0.0	0.0	12.531218183558568	4.3509663496262517E-4
56	0.0	0.0	0.0	13.426211961676689	4.3509663496262517E-4
57	0.0	0.0	0.0	14.329472575859098	4.3509663496262517E-4
58	0.0	0.0	0.0	15.1618124385426	4.3509663496262517E-4
59	0.0	0.0	0.0	15.958039280524204	4.3509663496262517E-4
60	0.0	0.0	0.0	16.74948005952122	4.3509663496262517E-4
61	0.0	0.0	0.0	17.488274145687758	4.3509663496262517E-4
62	0.0	0.0	0.0	18.214015332805417	4.3509663496262517E-4
63	0.0	0.0	0.0	18.923657944429458	4.3509663496262517E-4
64	0.0	0.0	0.0	19.63852171567305	4.3509663496262517E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGAAT	20	0.0069449972	52.5	66
GTTAAGC	35	0.0012536738	40.0	21
CGGTATG	35	0.0012536738	40.0	43
CGTCCAA	35	0.0012536738	40.0	40
ACTAATG	45	9.178936E-5	38.888885	13
CAAGTGC	40	0.0024154212	35.0	44
GCGGTAT	40	0.0024154212	35.0	42
GACGCTC	40	0.0024154212	35.0	58
CTTTAGA	40	0.0024154212	35.0	2
GCACTTT	80	6.2140316E-8	35.0	49
AGAGATC	50	1.7062368E-4	35.0	6
CTTACGT	40	0.0024154212	35.0	23
AAGAGAT	55	2.9860612E-4	31.81818	5
ACTTATG	55	2.9860612E-4	31.81818	48
TACGGAC	55	2.9860612E-4	31.81818	38
GCATAAG	45	0.004301361	31.111109	52
ATCTCAG	45	0.004301361	31.111109	52
AAGCGCG	45	0.004301361	31.111109	7
GAATAAG	45	0.004301361	31.111109	36
TTATCTT	45	0.004301361	31.111109	32
>>END_MODULE