FastQCFastQC Report
Wed 25 May 2016
SRR1781217_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781217_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences206908
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGT47272.2845902526726856No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCG4350.21023836681036984No Hit
CTTATACACATCTCCGAGCCCACGAGACAGACCAGCACCTCGTATGCCGT3510.16964061321940185No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCC3160.15272488255649855No Hit
CTTATACACATCTCCGAGCCCACGAGACAGACCAGCAACTCGTATGCCGT2430.1174435014595859No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTCTT150.002222319670.0000125
CCGGTAT150.002222319670.000014
TATGCCG6900.058.8405843
CCGTCTT6500.058.69230747
TCTTCTG6450.058.60465250
CGTATGC6950.058.41726741
CTCGTAT6800.058.16176639
TCTCGTA6100.057.9508238
TCGTATG6850.057.7372340
CATCTCG6350.056.2204736
GCATCTC6300.056.11111535
GTCTTCT6800.056.1029449
CTGCTTG6900.055.79710454
ATGCCGT7200.055.41666444
GCCGTCT6950.055.39568346
CGTCTTC6900.055.28985648
ATCTCGT6450.054.80620237
GTATGCC7500.054.59999542
CTTCTGC7000.054.551
CAGCATC6600.054.09090833
Produced by FastQC (version 0.11.5)