##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781217_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	206908
Sequences flagged as poor quality	0
Sequence length	76
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.28898834264504	34.0	34.0	34.0	33.0	34.0
2	33.293362267287876	34.0	34.0	34.0	33.0	34.0
3	33.337855471997216	34.0	34.0	34.0	33.0	34.0
4	33.39309258221045	34.0	34.0	34.0	33.0	34.0
5	33.4092833529878	34.0	34.0	34.0	34.0	34.0
6	36.860266398592614	38.0	37.0	38.0	34.0	38.0
7	36.75311732750788	38.0	37.0	38.0	34.0	38.0
8	36.539220329808415	38.0	37.0	38.0	34.0	38.0
9	36.50993678349798	38.0	37.0	38.0	34.0	38.0
10-11	36.40408539060839	38.0	37.0	38.0	34.0	38.0
12-13	36.34500840953467	38.0	37.0	38.0	34.0	38.0
14-15	36.34876128520888	38.0	37.0	38.0	34.0	38.0
16-17	36.28226071490711	38.0	37.0	38.0	34.0	38.0
18-19	36.277985868115294	38.0	37.0	38.0	34.0	38.0
20-21	36.2151342625708	38.0	37.0	38.0	33.5	38.0
22-23	36.28135451505017	38.0	37.0	38.0	34.0	38.0
24-25	36.292347323448105	38.0	37.0	38.0	34.0	38.0
26-27	36.06624200127593	38.0	37.0	38.0	32.5	38.0
28-29	36.06690171477179	38.0	37.0	38.0	32.5	38.0
30-31	36.090581321166894	38.0	37.0	38.0	33.0	38.0
32-33	36.0288437373132	38.0	37.0	38.0	32.0	38.0
34-35	36.03359705762948	38.0	37.0	38.0	32.0	38.0
36-37	35.9914744717459	38.0	37.0	38.0	32.0	38.0
38-39	35.775209271753624	38.0	36.0	38.0	31.0	38.0
40-41	35.91115374949253	38.0	36.5	38.0	31.5	38.0
42-43	35.97878525721577	38.0	37.0	38.0	32.0	38.0
44-45	35.96075067179616	38.0	37.0	38.0	32.0	38.0
46-47	35.95187957932994	38.0	37.0	38.0	32.0	38.0
48-49	35.94271125331065	38.0	37.0	38.0	32.0	38.0
50-51	35.83191080093568	38.0	36.0	38.0	31.0	38.0
52-53	35.845614959305586	38.0	36.5	38.0	31.0	38.0
54-55	35.8743523691689	38.0	37.0	38.0	32.0	38.0
56-57	35.83613973360141	38.0	36.0	38.0	31.0	38.0
58-59	35.81311259110329	38.0	36.0	38.0	31.0	38.0
60-61	35.79684932433739	38.0	36.0	38.0	31.0	38.0
62-63	35.78713727840393	38.0	36.0	38.0	31.0	38.0
64-65	35.750942447851216	38.0	36.0	38.0	31.0	38.0
66-67	35.700134842538716	38.0	36.0	38.0	31.0	38.0
68-69	35.571983683569506	38.0	36.0	38.0	31.0	38.0
70-71	35.36954588512769	38.0	36.0	38.0	29.5	38.0
72-73	35.21926411738551	38.0	36.0	38.0	27.0	38.0
74-75	35.23396388733157	38.0	36.0	38.0	27.0	38.0
76	34.29896379067025	37.0	34.0	38.0	25.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	8.0
22	28.0
23	80.0
24	156.0
25	348.0
26	609.0
27	1117.0
28	1846.0
29	2929.0
30	4313.0
31	6437.0
32	8697.0
33	12491.0
34	17213.0
35	23412.0
36	34715.0
37	92508.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.257312428712275	17.568194559901016	9.191524735631296	27.98296827575541
2	23.95605776480368	19.535735689291858	31.76290911902875	24.745297426875712
3	19.836835695091537	21.420631391729657	30.29462369748874	28.447909215690064
4	13.881531888568832	18.297987511357704	36.23881145243297	31.581669147640497
5	13.324762696464129	33.95035474703733	37.26970440968933	15.455178146809208
6	29.8093838807586	39.50064763083109	16.910414290409264	13.779554198001042
7	28.520405204245364	28.763508419200807	21.113248400255184	21.602837976298645
8	25.27935120923309	35.98942525180273	18.211475631681715	20.51974790728246
9	24.230575908133083	14.9771879289346	19.556517872677713	41.23571829025461
10-11	25.01836565043401	25.026581862470277	27.457614012024667	22.497438475071046
12-13	24.801844297948847	22.05835443772111	26.558905407234136	26.58089585709591
14-15	23.13636978753842	22.879492334757476	22.497921781661415	31.486216096042686
16-17	25.277901289461983	26.802975235370308	23.592611208846446	24.326512266321263
18-19	24.793386432617396	25.62757360759371	24.39248361590659	25.186556343882305
20-21	22.89544145223964	25.396311404102306	24.646219575850136	27.062027567807917
22-23	23.48580045237497	26.499942003209153	24.786136833761866	25.22812071065401
24-25	24.71726564463433	26.710422023314713	24.706391246351036	23.865921085699927
26-27	23.93189243528525	27.171013203936052	25.113577048736634	23.783517312042065
28-29	23.16198503682796	26.675623948808163	25.727376418504843	24.435014595859027
30-31	25.074187561621592	26.809499874340286	24.79362808591258	23.322684478125545
32-33	22.791288882015195	25.85593597154291	24.730314922574284	26.622460223867613
34-35	25.181239971388248	26.27327121232625	25.00724959885553	23.538239217429968
36-37	23.719720842113404	26.149786378487054	24.566473988439306	25.564018790960237
38-39	22.769781738743788	25.656330349720648	26.789200997544803	24.784686913990758
40-41	22.860885031028282	24.146480561408936	27.609130628105245	25.383503779457538
42-43	24.55680785663193	24.53940881937866	26.88682892879927	24.016954395190133
44-45	21.981025383262125	26.412221857057244	27.649728381696214	23.957024377984418
46-47	24.03242020608193	24.051752469696677	26.299853074796527	25.615974249424866
48-49	23.51431554120672	23.702563458155314	26.66402459063932	26.119096409998647
50-51	21.798335492102773	23.9649989367255	28.093887138245016	26.14277843292671
52-53	21.275397761323873	23.91932646393566	30.081485491136156	24.72379028360431
54-55	20.51974790728246	24.33931989096603	28.86186131034083	26.279070891410676
56-57	21.923028592417886	24.38378409727995	26.992431418794826	26.700755891507338
58-59	20.894552168113364	24.008254876563498	29.424188528234772	25.67300442708837
60-61	21.944052429098925	25.4395673439403	26.818199392966925	25.79818083399385
62-63	19.30930655170414	26.968749395866766	27.05743615519941	26.66450789722969
64-65	20.02653353181124	27.582065459044596	26.049258607690373	26.34214240145379
66-67	20.213814835579097	27.441181587952133	25.303758192046704	27.041245384422062
68-69	19.98472751174435	28.078662980648406	24.73732286813463	27.199286639472614
70-71	20.528689079204284	27.26912444178089	24.825043014286543	27.377143464728288
72-73	20.64226612794092	25.854244398476617	24.64887776209716	28.854611711485294
74-75	20.49630753764958	26.03161791714192	24.63244533802463	28.83962920718387
76	20.95617375838537	26.129004195101203	24.313221335086123	28.6016007114273
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.5
10	0.5
11	0.0
12	0.0
13	0.0
14	0.5
15	2.0
16	5.5
17	8.0
18	15.0
19	24.0
20	47.0
21	68.0
22	62.5
23	154.0
24	288.5
25	326.0
26	435.0
27	606.5
28	758.5
29	848.0
30	1100.5
31	1512.5
32	2248.5
33	2825.0
34	3201.5
35	4089.5
36	5303.5
37	6006.0
38	6503.5
39	7938.5
40	9941.5
41	11768.5
42	12530.0
43	13488.0
44	14669.0
45	14633.0
46	14374.0
47	14061.5
48	12658.5
49	11058.0
50	10548.0
51	9665.0
52	7692.0
53	6098.0
54	5594.0
55	5059.0
56	4148.5
57	3345.5
58	2918.0
59	2578.5
60	2218.5
61	2149.0
62	2100.0
63	2087.0
64	2124.5
65	2142.5
66	2160.0
67	2210.0
68	2187.5
69	2107.0
70	2055.5
71	2062.0
72	2056.5
73	1971.0
74	1833.5
75	1776.0
76	1681.0
77	1378.0
78	1102.5
79	1035.0
80	858.5
81	610.0
82	427.5
83	317.0
84	254.5
85	159.0
86	88.0
87	50.0
88	40.5
89	22.0
90	13.5
91	11.5
92	9.0
93	5.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	206908.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.509540472093875
#Duplication Level	Percentage of deduplicated	Percentage of total
1	59.67676428161282	20.594177122199238
2	18.125288853409522	12.509907785102559
3	7.359634749240228	7.6193283971620245
4	3.512457459770598	4.84853171457846
5	2.004117474055712	3.458058654087807
6	1.4061033850118343	2.9114389003808454
7	1.0447740291024186	2.5238270149051756
8	0.8346988221783399	2.3044058228778006
9	0.7058526952649048	2.1922786939122703
>10	5.107628531014103	33.170781216772674
>50	0.20587370278559725	4.552264774682468
>100	0.015405515174432446	1.0304096506659965
>500	0.0	0.0
>1k	0.0014005013794938588	2.2845902526726856
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCGT	4727	2.2845902526726856	No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCCG	435	0.21023836681036984	No Hit
CTTATACACATCTCCGAGCCCACGAGACAGACCAGCACCTCGTATGCCGT	351	0.16964061321940185	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGACCAGCATCTCGTATGCC	316	0.15272488255649855	No Hit
CTTATACACATCTCCGAGCCCACGAGACAGACCAGCAACTCGTATGCCGT	243	0.1174435014595859	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.00386645272294933	0.0
2	0.0	0.0	0.0	0.00386645272294933	0.0
3	0.0	0.0	0.0	0.00386645272294933	0.0
4	0.0	0.0	0.0	0.004833065903686663	0.0
5	0.0	0.0	0.0	0.0053163724940553285	0.0
6	0.0	0.0	0.0	0.0053163724940553285	0.0
7	0.0	0.0	0.0	0.0062829856747926615	0.0
8	0.0	0.0	0.0	0.0062829856747926615	0.0
9	0.0	0.0	0.0	0.008216212036267326	0.0
10	0.0	0.0	0.0	0.008216212036267326	0.0
11	0.0	0.0	0.0	0.008216212036267326	0.0
12	0.0	0.0	0.0	0.008216212036267326	0.0
13	0.0	0.0	0.0	0.008216212036267326	0.0
14	0.0	0.0	0.0	0.008699518626635993	0.0
15	0.0	0.0	0.0	0.009666131807373326	0.0
16	0.0	0.0	0.0	0.010149438397741992	0.0
17	0.0	0.0	0.0	0.010149438397741992	0.0
18	0.0	0.0	0.0	0.010632744988110657	0.0
19	0.0	0.0	0.0	0.012082664759216656	0.0
20	0.0	0.0	0.0	0.012082664759216656	0.0
21	0.0	0.0	0.0	0.012082664759216656	0.0
22	0.0	0.0	0.0	0.012565971349585323	0.0
23	0.0	0.0	0.0	0.01546581089179732	0.0
24	0.0	0.0	0.0	0.016432424072534653	0.0
25	0.0	0.0	0.0	0.017399037253271986	0.0
26	0.0	0.0	0.0	0.020298876795483985	0.0
27	0.0	0.0	0.0	0.032864848145069306	0.0
28	0.0	0.0	0.0	0.04881396562723529	0.0
29	0.0	0.0	0.0	0.07201268196493127	0.0
30	0.0	0.0	0.0	0.08989502580857192	0.0
31	0.0	0.0	0.0	0.11599358168847991	0.0
32	0.0	0.0	0.0	0.14692520347207455	0.0
33	0.0	0.0	0.0	0.18993949001488583	0.0
34	0.0	0.0	0.0	0.27886790264272043	0.0
35	0.0	0.0	0.0	0.40307769636746765	0.0
36	0.0	0.0	0.0	0.5823844413942428	0.0
37	0.0	0.0	0.0	0.8680186363021246	0.0
38	0.0	0.0	0.0	1.2039167166083478	0.0
39	0.0	0.0	0.0	1.603127960252866	0.0
40	0.0	0.0	0.0	2.0095888027529143	0.0
41	0.0	0.0	0.0	2.4455313472654514	0.0
42	0.0	0.0	0.0	2.963152705550293	0.0
43	0.0	0.0	0.0	3.557136505113384	0.0
44	0.0	0.0	0.0	4.232799118448779	0.0
45	0.0	0.0	0.0	5.066502986834728	0.0
46	0.0	0.0	0.0	6.0017012391980975	0.0
47	0.0	0.0	0.0	6.9248168268022505	0.0
48	0.0	0.0	0.0	7.797185222417693	0.0
49	0.0	0.0	0.0	8.670036924623505	0.0
50	0.0	0.0	0.0	9.393546890405398	0.0
51	0.0	0.0	0.0	10.12478976163319	0.0
52	0.0	0.0	0.0	10.851682873547663	0.0
53	0.0	0.0	0.0	11.590175343630985	0.0
54	0.0	0.0	0.0	12.397780656137027	0.0
55	0.0	0.0	0.0	13.279815183559842	0.0
56	0.0	0.0	0.0	14.221296421598005	0.0
57	0.0	0.0	0.0	15.19805904073308	0.0
58	0.0	0.0	0.0	16.080576874746264	0.0
59	0.0	0.0	0.0	16.926846714481798	0.0
60	0.0	0.0	0.0	17.759100663096643	0.0
61	0.0	0.0	0.0	18.526108222011715	0.0
62	0.0	0.0	0.0	19.298915460011212	0.0
63	0.0	0.0	0.0	20.05094051462486	0.0
64	0.0	0.0	0.0	20.779283546310438	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGTCTT	15	0.0022223196	70.00001	25
CCGGTAT	15	0.0022223196	70.00001	4
TATGCCG	690	0.0	58.84058	43
CCGTCTT	650	0.0	58.692307	47
TCTTCTG	645	0.0	58.604652	50
CGTATGC	695	0.0	58.417267	41
CTCGTAT	680	0.0	58.161766	39
TCTCGTA	610	0.0	57.95082	38
TCGTATG	685	0.0	57.73723	40
CATCTCG	635	0.0	56.22047	36
GCATCTC	630	0.0	56.111115	35
GTCTTCT	680	0.0	56.10294	49
CTGCTTG	690	0.0	55.797104	54
ATGCCGT	720	0.0	55.416664	44
GCCGTCT	695	0.0	55.395683	46
CGTCTTC	690	0.0	55.289856	48
ATCTCGT	645	0.0	54.806202	37
GTATGCC	750	0.0	54.599995	42
CTTCTGC	700	0.0	54.5	51
CAGCATC	660	0.0	54.090908	33
>>END_MODULE