FastQCFastQC Report
Wed 25 May 2016
SRR1781216_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781216_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189827
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGAA252.385937E-455.99999643
GACTGAC200.00694314352.57
ATCGAAT200.00694314352.568
CGAATAC305.8669376E-446.66666470
GGACTGA350.001253122240.06
GACCGGA350.001253122240.06
TCTATCG350.001253122240.031
TACCGTT400.00241436535.012
TGCCGAA501.7051188E-435.031
TCTATAC552.984115E-431.818183
TCTACTG450.00429949331.11110932
CAAATTC450.00429949331.11110940
TGGCTAA450.00429949331.11110964
GAGCACA604.9688155E-429.16666646
TCAATCC500.00719544927.99999848
TGGATGG500.00719544927.99999819
TAGTGAT500.00719544927.9999984
GACTGAG500.00719544927.9999987
TGAGAGC500.00719544927.99999810
ACCGAAC657.9347467E-426.92307712