##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1781216_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	189827
Sequences flagged as poor quality	0
Sequence length	76
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.206577567996124	34.0	32.0	34.0	23.0	34.0
2	31.25773994215786	34.0	32.0	34.0	23.0	34.0
3	31.236594372771	34.0	33.0	34.0	23.0	34.0
4	31.34737418807651	34.0	33.0	34.0	23.0	34.0
5	31.3935320054576	34.0	33.0	34.0	23.0	34.0
6	34.23587793095819	37.0	34.0	38.0	24.0	38.0
7	34.16667281261359	37.0	34.0	38.0	24.0	38.0
8	33.90503458412133	37.0	34.0	38.0	23.0	38.0
9	34.23488755551107	37.0	34.0	38.0	24.0	38.0
10-11	34.07572948000021	37.0	34.0	38.0	23.5	38.0
12-13	34.02282077891975	37.0	34.0	38.0	23.0	38.0
14-15	33.920648801276954	37.0	34.0	38.0	23.0	38.0
16-17	33.87251813493339	37.0	34.0	38.0	23.0	38.0
18-19	33.91948721730839	37.0	34.0	38.0	23.0	38.0
20-21	33.83099611751753	37.0	34.0	38.0	23.0	38.0
22-23	33.90008797484025	37.0	34.0	38.0	23.0	38.0
24-25	33.9182492479995	37.0	34.0	38.0	23.0	38.0
26-27	33.83768378576283	37.0	34.0	38.0	23.0	38.0
28-29	33.894825288288814	37.0	34.0	38.0	23.0	38.0
30-31	33.97358911008445	37.0	34.0	38.0	23.0	38.0
32-33	33.92152591570219	37.0	34.0	38.0	23.0	38.0
34-35	33.93053411790736	37.0	34.0	38.0	23.0	38.0
36-37	33.76861036628088	37.0	34.0	38.0	22.5	38.0
38-39	33.79000879748403	37.0	34.0	38.0	23.0	38.0
40-41	33.79243469053401	37.0	34.0	38.0	23.0	38.0
42-43	33.871172172557124	37.0	34.0	38.0	23.0	38.0
44-45	33.88286439758306	37.0	34.0	38.0	23.0	38.0
46-47	33.88432625495847	37.0	34.0	38.0	23.0	38.0
48-49	33.82882045230657	37.0	34.0	38.0	23.0	38.0
50-51	33.69584674466751	37.0	34.0	38.0	22.5	38.0
52-53	33.78211213367962	37.0	34.0	38.0	23.0	38.0
54-55	33.741127447623356	37.0	34.0	38.0	23.0	38.0
56-57	33.720911672206796	37.0	34.0	38.0	22.5	38.0
58-59	33.717018653826905	37.0	34.0	38.0	22.5	38.0
60-61	33.69550959557913	37.0	34.0	38.0	22.5	38.0
62-63	33.655768146786286	37.0	34.0	38.0	22.0	38.0
64-65	33.66983885327166	37.0	34.0	38.0	22.0	38.0
66-67	33.553509247894134	37.0	34.0	38.0	22.0	38.0
68-69	33.31716246898492	37.0	34.0	38.0	21.5	38.0
70-71	33.46208389744346	37.0	34.0	38.0	22.0	38.0
72-73	33.42364363341358	37.0	33.5	38.0	22.0	38.0
74-75	33.3957498143046	37.0	33.0	38.0	21.5	38.0
76	31.965853118892465	36.0	31.0	38.0	11.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	4.0
13	22.0
14	119.0
15	348.0
16	811.0
17	1363.0
18	1817.0
19	1888.0
20	1742.0
21	1730.0
22	1732.0
23	2010.0
24	2277.0
25	2658.0
26	3111.0
27	3632.0
28	4505.0
29	5363.0
30	6465.0
31	7619.0
32	9109.0
33	11151.0
34	14066.0
35	19076.0
36	28215.0
37	58993.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	45.56411890826911	18.95831467599446	9.75045699505339	25.727109420683043
2	23.28699289352937	19.208015719576245	31.41175912804817	26.09323225884621
3	20.048254463274457	20.98436997898086	28.53756314960464	30.429812408140045
4	14.477392573237738	16.12889631085146	35.148846054565475	34.24486506134533
5	14.504259141218057	33.454145090003	34.850153034078396	17.191442734700544
6	31.7815695343655	35.228392167605236	17.4632691872073	15.52676911082196
7	30.156932364732096	27.75000395096588	21.773509563971405	20.319554120330615
8	26.71906525415246	32.96159134369716	19.087906356840705	21.231437045309676
9	24.958514858265684	15.714835086684193	20.754687162521666	38.57196289252846
10-11	26.446975403920415	23.545649459771266	26.516512403398885	23.49086273290944
12-13	25.950997487185703	21.99871461910055	25.877772919553067	26.17251497416068
14-15	24.2115189093227	23.202442223708957	23.065738804279686	29.52030006268866
16-17	24.801793211713825	25.85749129470518	24.39379013522839	24.946925358352605
18-19	23.93231732050762	25.351504264409176	25.661259989358733	25.054918425724477
20-21	24.27341737476755	25.440269297834345	25.625437898718307	24.660875428679798
22-23	24.594235804179597	24.996180732983188	25.90595647615987	24.503626986677343
24-25	24.16489751194509	25.27617251497416	25.459760729506343	25.099169243574416
26-27	23.56724807324564	25.457390149978664	26.114040679144697	24.861321097631002
28-29	24.202563386662593	25.176344777086506	26.458038108382897	24.163053727868007
30-31	24.337159624289484	25.080204607353014	25.807709124624	24.774926643733505
32-33	23.642843220405947	25.52060560405	25.927818487359545	24.908732688184504
34-35	24.523645213799934	24.715662155541622	26.165403235577656	24.595289395080783
36-37	24.55182877040674	24.681157053527684	25.618589557860577	25.148424618204995
38-39	23.820699428162033	25.1023961354184	26.605347514704214	24.471556921715358
40-41	24.017921581229224	24.180437977737622	27.014070706485377	24.787569734547773
42-43	23.623088391008658	24.4217102941099	26.70247119745874	25.252730117422708
44-45	22.90770016910134	24.124070864524015	27.476860509832633	25.491368456542006
46-47	23.125003292471565	24.049792705990193	27.414171851211893	25.411032150326353
48-49	22.559224978533084	23.913352684286217	28.04158523286993	25.485837104310765
50-51	22.2997782191153	24.199402613959027	27.910939961122494	25.58987920580318
52-53	22.060613084545402	24.111691171435044	28.108224857370136	25.71947088664942
54-55	21.510111838674163	24.715398757816327	28.08188508484041	25.692604318669105
56-57	21.26567874959832	24.722247098674053	28.02630816480269	25.985765986924935
58-59	21.66130213299478	24.598976963234946	27.57010330458786	26.169617599182416
60-61	21.21695017041833	24.349802715103753	27.7579058827248	26.675341231753123
62-63	21.143725602785697	23.981572695138205	28.07003218720203	26.804669514874067
64-65	21.86885954052901	23.975251149731072	27.385461499154495	26.770427810585428
66-67	21.946561869491696	23.94548720677248	26.62476886849605	27.48318205523977
68-69	21.921275687863158	24.055060660496135	26.480163517307865	27.543500134332838
70-71	22.135944833980414	24.12064669409515	26.467783824218895	27.27562464770554
72-73	22.352194366449453	23.735295821985282	25.97391308928656	27.938596722278707
74-75	22.73754523855932	23.93521469548589	25.905166282983984	27.422073782970813
76	22.911387737255502	23.552497800628995	25.79085167020498	27.74526279191053
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	1.0
12	2.0
13	2.0
14	3.0
15	7.5
16	22.0
17	33.0
18	39.0
19	55.5
20	80.5
21	95.0
22	129.0
23	211.5
24	364.0
25	468.0
26	545.5
27	727.5
28	1005.0
29	1178.0
30	1383.0
31	1745.0
32	2364.0
33	2826.0
34	3229.0
35	4002.0
36	4995.0
37	5618.0
38	6173.5
39	7487.5
40	8981.5
41	10129.5
42	10542.0
43	10820.0
44	11255.5
45	11355.0
46	11297.0
47	11136.5
48	10399.5
49	9409.0
50	8995.0
51	8273.0
52	6777.5
53	5593.0
54	5182.0
55	4817.0
56	4151.5
57	3561.0
58	3271.0
59	2964.0
60	2602.5
61	2457.0
62	2366.0
63	2463.0
64	2495.5
65	2437.5
66	2407.0
67	2370.0
68	2352.0
69	2338.5
70	2296.5
71	2250.0
72	2227.0
73	2121.0
74	1928.5
75	1819.0
76	1714.0
77	1461.5
78	1185.0
79	1056.0
80	971.5
81	736.5
82	500.5
83	415.0
84	360.5
85	241.0
86	144.5
87	113.0
88	84.0
89	48.5
90	28.5
91	12.5
92	10.0
93	7.0
94	4.0
95	2.0
96	0.0
97	1.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	2.633977252972443E-4
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
76	189827.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.55399280776511
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.28271981483553	39.24582249897168
2	13.626947899468686	14.59554939599872
3	5.029355960236146	8.080262787665315
4	2.2229984801138993	4.762017784627702
5	1.2199954967213573	3.2667815028460696
6	0.8299968312493136	2.6669786598716136
7	0.6339975761564344	2.376717114353554
8	0.44499829862209034	1.9065148547100108
9	0.43099835214452437	2.0773514385815828
>10	2.256991370741976	20.294743520612325
>50	0.020999919710049345	0.727260441761439
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.267954505944886E-4
2	0.0	0.0	0.0	0.0	5.267954505944886E-4
3	0.0	0.0	0.0	0.0	5.267954505944886E-4
4	0.0	0.0	0.0	0.0	5.267954505944886E-4
5	0.0	0.0	0.0	0.0	5.267954505944886E-4
6	0.0	0.0	0.0	0.0	5.267954505944886E-4
7	0.0	0.0	0.0	0.0	5.267954505944886E-4
8	0.0	0.0	0.0	5.267954505944886E-4	5.267954505944886E-4
9	0.0	0.0	0.0	5.267954505944886E-4	5.267954505944886E-4
10	0.0	0.0	0.0	5.267954505944886E-4	5.267954505944886E-4
11	0.0	0.0	0.0	5.267954505944886E-4	5.267954505944886E-4
12	0.0	0.0	0.0	5.267954505944886E-4	5.267954505944886E-4
13	0.0	0.0	0.0	5.267954505944886E-4	5.267954505944886E-4
14	0.0	0.0	0.0	0.0010535909011889773	5.267954505944886E-4
15	0.0	0.0	0.0	0.0010535909011889773	5.267954505944886E-4
16	0.0	0.0	0.0	0.0010535909011889773	5.267954505944886E-4
17	0.0	0.0	0.0	0.0010535909011889773	5.267954505944886E-4
18	0.0	0.0	0.0	0.001580386351783466	5.267954505944886E-4
19	0.0	0.0	0.0	0.001580386351783466	5.267954505944886E-4
20	0.0	0.0	0.0	0.001580386351783466	5.267954505944886E-4
21	0.0	0.0	0.0	0.001580386351783466	5.267954505944886E-4
22	0.0	0.0	0.0	0.004214363604755909	5.267954505944886E-4
23	0.0	0.0	0.0	0.004214363604755909	5.267954505944886E-4
24	0.0	0.0	0.0	0.005267954505944887	5.267954505944886E-4
25	0.0	0.0	0.0	0.005267954505944887	5.267954505944886E-4
26	0.0	0.0	0.0	0.00790193175891733	5.267954505944886E-4
27	0.0	0.0	0.0	0.022125408924968525	5.267954505944886E-4
28	0.0	0.0	0.0	0.04056324969577563	5.267954505944886E-4
29	0.0	0.0	0.0	0.07059059037966148	5.267954505944886E-4
30	0.0	0.0	0.0	0.09008202205165756	5.267954505944886E-4
31	0.0	0.0	0.0	0.10851986282246466	5.267954505944886E-4
32	0.0	0.0	0.0	0.1448687489134844	5.267954505944886E-4
33	0.0	0.0	0.0	0.19860188487412223	5.267954505944886E-4
34	0.0	0.0	0.0	0.27235324795735066	5.267954505944886E-4
35	0.0	0.0	0.0	0.3950965879458665	5.267954505944886E-4
36	0.0	0.0	0.0	0.5926448819187997	5.267954505944886E-4
37	0.0	0.0	0.0	0.9129365158802488	5.267954505944886E-4
38	0.0	0.0	0.0	1.275371785889257	5.267954505944886E-4
39	0.0	0.0	0.0	1.7073440553767378	5.267954505944886E-4
40	0.0	0.0	0.0	2.245202210433711	5.267954505944886E-4
41	0.0	0.0	0.0	2.7735780473799827	5.267954505944886E-4
42	0.0	0.0	0.0	3.373071270156511	5.267954505944886E-4
43	0.0	0.0	0.0	4.122174400901874	5.267954505944886E-4
44	0.0	0.0	0.0	4.912367576793607	5.267954505944886E-4
45	0.0	0.0	0.0	5.777365706669757	5.267954505944886E-4
46	0.0	0.0	0.0	6.730338676795188	5.267954505944886E-4
47	0.0	0.0	0.0	7.694374351383101	5.267954505944886E-4
48	0.0	0.0	0.0	8.580971094733625	5.267954505944886E-4
49	0.0	0.0	0.0	9.433852929246102	5.267954505944886E-4
50	0.0	0.0	0.0	10.273564877493717	5.267954505944886E-4
51	0.0	0.0	0.0	11.071133189693773	5.267954505944886E-4
52	0.0	0.0	0.0	11.853951229277184	5.267954505944886E-4
53	0.0	0.0	0.0	12.65046595057605	5.267954505944886E-4
54	0.0	0.0	0.0	13.460677353590375	5.267954505944886E-4
55	0.0	0.0	0.0	14.349381278743277	5.267954505944886E-4
56	0.0	0.0	0.0	15.224388522180723	5.267954505944886E-4
57	0.0	0.0	0.0	16.01879606167721	5.267954505944886E-4
58	0.0	0.0	0.0	16.84270414640699	5.267954505944886E-4
59	0.0	0.0	0.0	17.60076279981246	5.267954505944886E-4
60	0.0	0.0	0.0	18.379366475791116	5.267954505944886E-4
61	0.0	0.0	0.0	19.14163949280134	5.267954505944886E-4
62	0.0	0.0	0.0	19.874411964578275	5.267954505944886E-4
63	0.0	0.0	0.0	20.576630300220728	5.267954505944886E-4
64	0.0	0.0	0.0	21.32573343096609	5.267954505944886E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCCGAA	25	2.385937E-4	55.999996	43
GACTGAC	20	0.006943143	52.5	7
ATCGAAT	20	0.006943143	52.5	68
CGAATAC	30	5.8669376E-4	46.666664	70
GGACTGA	35	0.0012531222	40.0	6
GACCGGA	35	0.0012531222	40.0	6
TCTATCG	35	0.0012531222	40.0	31
TACCGTT	40	0.002414365	35.0	12
TGCCGAA	50	1.7051188E-4	35.0	31
TCTATAC	55	2.984115E-4	31.81818	3
TCTACTG	45	0.004299493	31.111109	32
CAAATTC	45	0.004299493	31.111109	40
TGGCTAA	45	0.004299493	31.111109	64
GAGCACA	60	4.9688155E-4	29.166666	46
TCAATCC	50	0.007195449	27.999998	48
TGGATGG	50	0.007195449	27.999998	19
TAGTGAT	50	0.007195449	27.999998	4
GACTGAG	50	0.007195449	27.999998	7
TGAGAGC	50	0.007195449	27.999998	10
ACCGAAC	65	7.9347467E-4	26.923077	12
>>END_MODULE