FastQCFastQC Report
Wed 25 May 2016
SRR1781216_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781216_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172109
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCGT42622.476337669732553No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCCG4160.2417072901475228No Hit
CTTATACACATCTCCGAGCCCACGAGACGACTTAGTACCTCGTATGCCGT2650.15397219204109025No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACTTAGTATCTCGTATGCC2060.11969159079420601No Hit
CTTATACACATCTCCGAGCCCACGAGACGACTTAGTAACTCGTATGCCGT1810.10516591229976352No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCTCG207.909507E-570.036
CGCTACA252.3853096E-455.99999663
CCGTCTT6150.055.20325547
CGTCTTC6050.054.95868348
TATGCCG6500.054.92307743
CGTGCGA452.685556E-854.44444710
CTCGTAT6250.054.31999639
CGTATGC6600.054.09090841
TCGTATG6450.053.7209340
TCTCGTA5800.053.70689838
GTATCTC5750.053.56521635
GCCGTCT6350.053.46456546
ATGCCGT6600.053.03030444
GTATGCC6750.052.8888942
CTTGAAA6050.052.6446357
CGGAATG200.006942047752.54
GAATCCA200.006942047752.534
CGAGAAT407.446888E-752.514
ATCTCGT6000.051.91666837
CTGCTTG6300.051.66666854